RT Journal Article SR Electronic(1) A1 Venturi, Giulietta A1 Ciccozzi, Massimo A1 Montieri, Stefania A1 Bartoloni, Alessandro A1 Francisci, Daniela A1 Nicoletti, Loredana A1 Fortuna, Claudia A1 Marongiu, Luigi A1 Rezza, Giovanni A1 Ciufolini, Maria GraziaYR 2007 T1 Genetic variability of the M genome segment of clinical and environmental Toscana virus strains JF Journal of General Virology, VO 88 IS 4 SP 1288 OP 1294 DO https://doi.org/10.1099/vir.0.82330-0 PB Microbiology Society, SN 1465-2099, AB Twenty-seven strains of Toscana virus, collected over a period of 23 years and isolated from several localities and from different hosts (humans, arthropods and a bat), were investigated by sequencing of a portion of the M genomic segment comprising the GN glycoprotein coding region. Sequence data indicated that the divergence among isolates ranged from 0 to 5.7 % at the nucleotide level and from 0 to 3.4 % at the amino acid level. Phylogenetic analysis revealed four main clusters. A close correspondence between viral strains and area/year of isolation could not be demonstrated, whilst co-circulation of different viral strains in the same area and in the same time period was observed for both patients and environmental viral isolates. Alignment of the deduced amino acid sequences and evolutionary analysis indicated that most of the sites along the gene may be invariable because of purifying and/or neutral selection., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82330-0