1887

Abstract

(OvHV-2) is endemic in sheep populations worldwide and causes malignant catarrhal fever (MCF), a lymphoproliferative disease, in cattle, bison and deer. OvHV-2 has been placed in the gammaherpesvirus subfamily and is related closely to (AlHV-1). Here, the cloning, sequencing and analysis of the complete OvHV-2 genome derived from a lymphoblastoid cell line from an affected cow (BJ1035) are reported. The unique portion of the genome consists of 130 930 bp, with a mean G+C content of 52 mol%. The unique DNA is flanked by multiple copies of terminal repeat elements 4205 bp in length, with a mean G+C content of 72 mol%. Analysis revealed 73 open reading frames (ORFs), the majority (62) of which showed homology to other gammaherpesvirus genes. A further subset of nine ORFs is shared with only the related AlHV-1. Three ORFs are entirely unique to OvHV-2, including a spliced homologue of cellular interleukin-10 that retains the exon structure of the cellular gene. The sequence of OvHV-2 is a critical first step in the study of the pathogenesis and treatment of MCF.

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2007-01-01
2024-03-28
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References

  1. Albini S., Zimmermann W., Neff F., Ehlers B., Häni H., Li H., Hüssy D., Engels M., Ackermann M. 2003; Identification and quantification of ovine gammaherpesvirus 2 DNA in fresh and stored tissues of pigs with symptoms of porcine malignant catarrhal fever. J Clin Microbiol 41:900–904 [CrossRef]
    [Google Scholar]
  2. Albrecht J.-C., Nicholas J., Biller D., Cameron K. R., Biesinger B., Newman C., Wittmann S., Craxton M. A., Coleman H. other authors 1992; Primary structure of the herpesvirus saimiri genome. J Virol 66:5047–5058
    [Google Scholar]
  3. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  4. AuCoin D. P., Pari G. S. 2002; The human herpesvirus-8 (Kaposi's sarcoma-associated herpesvirus) ORF 40/41 region encodes two distinct transcripts. J Gen Virol 83:189–193
    [Google Scholar]
  5. AuCoin D. P., Colletti K. S., Xu Y., Cei S. A., Pari G. S. 2002; Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) contains two functional lytic origins of DNA replication. J Virol 76:7890–7896 [CrossRef]
    [Google Scholar]
  6. Baxter S. I. F., Pow I., Bridgen A., Reid H. W. 1993; PCR detection of the sheep-associated agent of malignant catarrhal fever. Arch Virol 132:145–159 [CrossRef]
    [Google Scholar]
  7. Baxter S. I. F., Wiyono A., Pow I., Reid H. W. 1997; Identification of ovine herpesvirus-2 infection in sheep. Arch Virol 142:823–831 [CrossRef]
    [Google Scholar]
  8. Beisser P. S., Verzijl D., Gruijthuijsen Y. K., Beuken E., Smit M. J., Leurs R., Bruggeman C. A., Vink C. 2005; The Epstein-Barr virus BILF1 gene encodes a G protein-coupled receptor that inhibits phosphorylation of RNA-dependent protein kinase. J Virol 79:441–449 [CrossRef]
    [Google Scholar]
  9. Bellows D. S., Howell M., Pearson C., Hazlewood S. A., Hardwick J. M. 2002; Epstein-Barr virus BALF1 is a BCL-2-like antagonist of the herpesvirus antiapoptotic BCL-2 proteins. J Virol 76:2469–2479 [CrossRef]
    [Google Scholar]
  10. Besemer J., Lomsadze A., Borodovsky M. 2001; GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Nucleic Acids Res 29:2607–2618 [CrossRef]
    [Google Scholar]
  11. Birkmann A., Mahr K., Ensser A., Yağuboğlu S., Titgemeyer F., Fleckenstein B., Neipel F. 2001; Cell surface heparan sulfate is a receptor for human herpesvirus 8 and interacts with envelope glycoprotein K8.1. J Virol 75:11583–11593 [CrossRef]
    [Google Scholar]
  12. Bortz E., Whitelegge J. P., Jia Q., Zhou Z. H., Stewart J. P., Wu T.-T., Sun R. 2003; Identification of proteins associated with murine gammaherpesvirus 68 virions. J Virol 77:13425–13432 [CrossRef]
    [Google Scholar]
  13. Bridgen A., Reid H. W. 1991; Derivation of a DNA clone corresponding to the viral agent of sheep-associated malignant catarrhal fever. Res Vet Sci 50:38–44 [CrossRef]
    [Google Scholar]
  14. Buxton D., Reid H. W., Finlayson J., Pow I. 1984; Pathogenesis of ‘sheep-associated’ malignant catarrhal fever in rabbits. Res Vet Sci 36:205–211
    [Google Scholar]
  15. Buxton D., Jacoby R. O., Reid H. W., Goodall P. A. 1988; The pathology of “sheep-associated” malignant catarrhal fever in the hamster. J Comp Pathol 98:155–166 [CrossRef]
    [Google Scholar]
  16. Comeau M. R., Johnson R., DuBose R. F., Petersen M., Gearing P., VandenBos T., Park L., Farrah T., Buller R. M. other authors 1998; A poxvirus-encoded semaphorin induces cytokine production from monocytes and binds to a novel cellular semaphorin receptor, VESPR. Immunity 8:473–482 [CrossRef]
    [Google Scholar]
  17. Coulter L. J., Reid H. W. 2002; Isolation and expression of three open reading frames from ovine herpesvirus-2. J Gen Virol 83:533–543
    [Google Scholar]
  18. Cunningham C., Davison A. J. 1993; A cosmid-based system for constructing mutants of herpes simplex virus type 1. Virology 197:116–124 [CrossRef]
    [Google Scholar]
  19. Deng H., Chu J. T., Park N.-H., Sun R. 2004; Identification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68. J Virol 78:9123–9131 [CrossRef]
    [Google Scholar]
  20. Devon R. S., Porteous D. J., Brookes A. J. 1995; Splinkerettes – improved vectorettes for greater efficiency in PCR walking. Nucleic Acids Res 23:1644–1645 [CrossRef]
    [Google Scholar]
  21. Ensser A., Pflanz R., Fleckenstein B. 1997; Primary structure of the alcelaphine herpesvirus 1 genome. J Virol 71:6517–6525
    [Google Scholar]
  22. Ensser A., Thurau M., Wittmann S., Fickenscher H. 2003; The genome of herpesvirus saimiri C488 which is capable of transforming human T cells. Virology 314:471–487 [CrossRef]
    [Google Scholar]
  23. Fixman E. D., Hayward G. S., Hayward S. D. 1995; Replication of Epstein-Barr virus oriLyt: lack of a dedicated virally encoded origin-binding protein and dependence on Zta in cotransfection assays. J Virol 69:2998–3006
    [Google Scholar]
  24. Gao S.-J., Zhang Y.-J., Deng J.-H., Rabkin C. S., Flore O., Jenson H. B. 1999; Molecular polymorphism of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) latent nuclear antigen: evidence for a large repertoire of viral genotypes and dual infection with different viral genotypes. J Infect Dis 180:1466–1476 [CrossRef]
    [Google Scholar]
  25. Gish W., States D. J. 1993; Identification of protein coding regions by database similarity search. Nat Genet 3:266–272 [CrossRef]
    [Google Scholar]
  26. Goltz M., Ericsson T., Patience C., Huang C. A., Noack S., Sachs D. H., Ehlers B. 2002; Sequence analysis of the genome of porcine lymphotropic herpesvirus 1 and gene expression during posttransplant lymphoproliferative disease of pigs. Virology 294:383–393 [CrossRef]
    [Google Scholar]
  27. Hamilton A. F. 1990; Account of three outbreaks of malignant catarrhal fever in cattle in the Republic of Ireland. Vet Rec 127:231–232
    [Google Scholar]
  28. Holmes S., Downs A.-M., Fosberry A., Hayes P. D., Michalovich D., Murdoch P., Moores K., Fox J., Deen K. other authors 2002; Sema7A is a potent monocyte stimulator. Scand J Immunol 56:270–275 [CrossRef]
    [Google Scholar]
  29. Hong G. K., Delecluse H.-J., Gruffat H., Morrison T. E., Feng W.-H., Sergeant A., Kenney S. C. 2004; The BRRF1 early gene of Epstein-Barr virus encodes a transcription factor that enhances induction of lytic infection by BRLF1. J Virol 78:4983–4992 [CrossRef]
    [Google Scholar]
  30. Hu J., Garber A. C., Renne R. 2002; The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus supports latent DNA replication in dividing cells. J Virol 76:11677–11687 [CrossRef]
    [Google Scholar]
  31. Hüssy D., Janett F., Albini S., Stäuber N., Thun R., Ackermann M. 2002; Analysis of the pathogenetic basis for shedding and transmission of ovine gamma herpesvirus 2. J Clin Microbiol 40:4700–4704 [CrossRef]
    [Google Scholar]
  32. Li H., Snowder G., O'Toole D., Crawford T. B. 1998; Transmission of ovine herpesvirus 2 in lambs. J Clin Microbiol 36:223–226
    [Google Scholar]
  33. Li H., Taus N. S., Lewis G. S., Kim O., Traul D. L., Crawford T. B. 2004; Shedding of ovine herpesvirus 2 in sheep nasal secretions: the predominant mode for transmission. J Clin Microbiol 42:5558–5564 [CrossRef]
    [Google Scholar]
  34. Li H., Taus N. S., Jones C., Murphy B., Evermann J. F., Crawford T. B. 2006; A devastating outbreak of malignant catarrhal fever in a bison feedlot. J Vet Diagn Invest 18:119–123 [CrossRef]
    [Google Scholar]
  35. Lin C. L., Li H., Wang Y., Zhu F. X., Kudchodkar S., Yuan Y. 2003; Kaposi's sarcoma-associated herpesvirus lytic origin ( ori-Lyt )-dependent DNA replication: identification of the ori-Lyt and association of K8 bZip protein with the origin. J Virol 77:5578–5588 [CrossRef]
    [Google Scholar]
  36. Loken T., Aleksandersen M., Reid H., Pow I. 1998; Malignant catarrhal fever caused by ovine herpesvirus-2 in pigs in Norway. Vet Rec 143:464–467 [CrossRef]
    [Google Scholar]
  37. Marshall W. L., Yim C., Gustafson E., Graf T., Sage D. R., Hanify K., Williams L., Fingeroth J., Finberg R. W. 1999; Epstein-Barr virus encodes a novel homolog of the bcl-2 oncogene that inhibits apoptosis and associates with Bax and Bak. J Virol 73:5181–5185
    [Google Scholar]
  38. May J. S., Coleman H. M., Boname J. M., Stevenson P. G. 2005; Murine gammaherpesvirus-68 ORF28 encodes a non-essential virion glycoprotein. J Gen Virol 86:919–928 [CrossRef]
    [Google Scholar]
  39. McGeoch D. J., Rixon F. J., Davison A. J. 2006; Topics in herpesvirus genomics and evolution. Virus Res 117:90–104 [CrossRef]
    [Google Scholar]
  40. Müller-Doblies U. U., Li H., Hauser B., Adler H., Ackermann M. 1998; Field validation of laboratory tests for clinical diagnosis of sheep-associated malignant catarrhal fever. J Clin Microbiol 36:2970–2972
    [Google Scholar]
  41. Müller-Doblies U. U., Egli J., Hauser B., Li H., Strasser M., Ehrensperger F., Braun U., Ackermann M. 2001; Malignant catarrhal fever in Switzerland. 2. Evaluation of the diagnosis. Schweiz Arch Tierheilkd 143:581–591 (in German
    [Google Scholar]
  42. O'Toole D., Li H., Sourk C., Montgomery D. L., Crawford T. B. 2002; Malignant catarrhal fever in a bison ( Bison bison ) feedlot, 1993-2000. J Vet Diagn Invest 14:183–193 [CrossRef]
    [Google Scholar]
  43. Paulsen S. J., Rosenkilde M. M., Eugen-Olsen J., Kledal T. N. 2005; Epstein-Barr virus-encoded BILF1 is a constitutively active G protein-coupled receptor. J Virol 79:536–546 [CrossRef]
    [Google Scholar]
  44. Plowright W., Ferris R. D., Scott G. R. 1960; Blue wildebeest and the aetiological agent of bovine malignant catarrhal fever. Nature 188:1167–1169 [CrossRef]
    [Google Scholar]
  45. Reid H. W., Buxton D., Pow I., Finlayson J., Berrie E. L. 1983; A cytotoxic T-lymphocyte line propagated from a rabbit infected with sheep associated malignant catarrhal fever. Res Vet Sci 34:109–113
    [Google Scholar]
  46. Reid H. W., Buxton D., Pow I., Finlayson J. 1989; Isolation and characterisation of lymphoblastoid cells from cattle and deer affected with ‘sheep-associated’ malignant catarrhal fever. Res Vet Sci 47:90–96
    [Google Scholar]
  47. Rice P., Longden I., Bleasby A. 2000; emboss: the European Molecular Biology Open Software Suite. Trends Genet 16:276–277 [CrossRef]
    [Google Scholar]
  48. Rosbottom J., Dalziel R. G., Reid H. W., Stewart J. P. 2002; Ovine herpesvirus 2 lytic cycle replication and capsid production. J Gen Virol 83:2999–3002
    [Google Scholar]
  49. Rutherford K., Parkhill J., Crook J., Horsnell T., Rice P., Rajandream M.-A., Barrell B. 2000; Artemis: sequence visualization and annotation. Bioinformatics 16:944–945 [CrossRef]
    [Google Scholar]
  50. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  51. Schock A., Reid H. W. 1996; Characterisation of the lymphoproliferation in rabbits experimentally affected with malignant catarrhal fever. Vet Microbiol 53:111–119 [CrossRef]
    [Google Scholar]
  52. Schock A., Collins R. A., Reid H. W. 1998; Phenotype, growth regulation and cytokine transcription in Ovine herpesvirus-2 (OHV-2)-infected bovine T-cell lines. Vet Immunol Immunopathol 66:67–81 [CrossRef]
    [Google Scholar]
  53. Schultheiss P. C., Collins J. K., Spraker T. R., DeMartini J. C. 2000; Epizootic malignant catarrhal fever in three bison herds: differences from cattle and association with ovine herpesvirus-2. J Vet Diagn Invest 12:497–502 [CrossRef]
    [Google Scholar]
  54. Simon S., Li H., O'Toole D., Crawford T. B., Oaks J. L. 2003; The vascular lesions of a cow and bison with sheep-associated malignant catarrhal fever contain ovine herpesvirus 2-infected CD8+ T lymphocytes. J Gen Virol 84:2009–2013 [CrossRef]
    [Google Scholar]
  55. Spriggs M. K., Armitage R. J., Comeau M. R., Strockbine L., Farrah T., Macduff B., Ulrich D., Alderson M. R., Müllberg J., Cohen J. I. 1996; The extracellular domain of the Epstein-Barr virus BZLF2 protein binds the HLA-DR β chain and inhibits antigen presentation. J Virol 70:5557–5563
    [Google Scholar]
  56. Stewart J. P., Silvia O. J., Atkin I. M. D., Hughes D. J., Ebrahimi B., Adler H. 2004; In vivo function of a gammaherpesvirus virion glycoprotein: influence on B-cell infection and mononucleosis. J Virol 78:10449–10459 [CrossRef]
    [Google Scholar]
  57. Swa S., Wright H., Thomson J., Reid H., Haig D. 2001; Constitutive activation of Lck and Fyn tyrosine kinases in large granular lymphocytes infected with the γ -herpesvirus agents of malignant catarrhal fever. Immunology 102:44–52 [CrossRef]
    [Google Scholar]
  58. Tanner J., Weis J., Fearon D., Whang Y., Kieff E. 1987; Epstein-Barr virus gp350/220 binding to the B lymphocyte C3d receptor mediates adsorption, capping, and endocytosis. Cell 50:203–213 [CrossRef]
    [Google Scholar]
  59. Taus N. S., Oaks J. L., Gailbreath K., Traul D. L., O'Toole D., Li H. 2006; Experimental aerosol infection of cattle ( Bos taurus ) with ovine herpesvirus 2 using nasal secretions from infected sheep. Vet Microbiol 116:29–36 [CrossRef]
    [Google Scholar]
  60. Taus N. S., Herndon D. R., Traul D. L., Stewart J. P., Ackermann M., Li H., Knowles D. P., Lewis G. S., Brayton K. A. 2007; Comparison of ovine herpesvirus 2 genomes isolated from domestic sheep ( Ovis aries ) and a clinically affected cow ( Bos bovis ). J Gen Virol 88:40–45 [CrossRef]
    [Google Scholar]
  61. Thonur L., Russell G. C., Stewart J. P., Haig D. M. 2006; Differential transcription of ovine herpesvirus 2 genes in lymphocytes from reservoir and susceptible species. Virus Genes 32:27–35 [CrossRef]
    [Google Scholar]
  62. Wiyono A., Baxter S. I. F., Saepulloh M., Damayanti R., Daniels P., Reid H. W. 1994; PCR detection of ovine herpesvirus-2 DNA in Indonesian ruminants – normal sheep and clinical cases of malignant catarrhal fever. Vet Microbiol 42:45–52 [CrossRef]
    [Google Scholar]
  63. Wu F. Y., Ahn J.-H., Alcendor D. J., Jang W.-J., Xiao J., Hayward S. D., Hayward G. S. 2001; Origin-independent assembly of Kaposi's sarcoma-associated herpesvirus DNA replication compartments in transient cotransfection assays and association with the ORF-K8 protein and cellular PML. J Virol 75:1487–1506 [CrossRef]
    [Google Scholar]
  64. Zhang Y.-J., Deng J.-H., Rabkin C., Gao S.-J. 2000; Hot-spot variations of Kaposi's sarcoma-associated herpesvirus latent nuclear antigen and application in genotyping by PCR–RFLP. J Gen Virol 81:2049–2058
    [Google Scholar]
  65. Zimmermann W., Broll H., Ehlers B., Buhk H. J., Rosenthal A., Goltz M. 2001; Genome sequence of bovine herpesvirus 4, a bovine Rhadinovirus , and identification of an origin of DNA replication. J Virol 75:1186–1194 [CrossRef]
    [Google Scholar]
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