1887

Abstract

Proteins of the virion tegument of alphaherpesviruses are involved in protein–protein interactions, which play important roles in virus morphogenesis. Seven single-gene deletion mutants of Pseudorabies virus were analysed for alterations in the overall composition of the virion beyond the loss of the targeted protein. The UL36 protein (pUL36) was present in equal amounts in wild-type virions and mutants lacking pUL21, pUL49, pUL51, pUS3 or pUS8. Virions lacking pUL11 or pUL16 incorporated less full-length pUL36 than wild-type particles, but contained increased amounts of an N-terminal fragment of pUL36 that is present only in traces in wild-type virus and the other mutants.

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2006-12-01
2020-01-24
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References

  1. Baskerville, A. ( 1973; ). The histopathology of experimental pneumonia in pigs produced by Aujeszky's disease virus. Res Vet Sci 14, 223–228.
    [Google Scholar]
  2. Fuchs, W., Klupp, B. G., Granzow, H., Hengartner, C., Brack, A., Mundt, A., Enquist, L. W. & Mettenleiter, T. C. ( 2002; ). Physical interaction between envelope glycoproteins E and M of pseudorabies virus and the major tegument protein UL49. J Virol 76, 8208–8217.[CrossRef]
    [Google Scholar]
  3. Fuchs, W., Klupp, B. G., Granzow, H. & Mettenleiter, T. C. ( 2004; ). Essential function of the pseudorabies virus UL36 gene product is independent of its interaction with the UL37 protein. J Virol 78, 11879–11889.[CrossRef]
    [Google Scholar]
  4. Kaplan, A. S. & Vatter, A. E. ( 1959; ). A comparison of herpes simplex and pseudorabies viruses. Virology 7, 394–407.[CrossRef]
    [Google Scholar]
  5. Karger, A., Bettin, B., Granzow, H. & Mettenleiter, T. C. ( 1998; ). Simple and rapid purification of alphaherpesviruses by chromatography on a cation exchange membrane. J Virol Methods 70, 219–224.[CrossRef]
    [Google Scholar]
  6. Kattenhorn, L. M., Korbel, G. A., Kessler, B. M., Spooner, E. & Ploegh, H. L. ( 2005; ). A deubiquitinating enzyme encoded by HSV-1 belongs to a family of cysteine proteases that is conserved across the family Herpesviridae. Mol Cell 19, 547–557.[CrossRef]
    [Google Scholar]
  7. Klupp, B. G., Granzow, H. & Mettenleiter, T. C. ( 2001; ). Effect of the pseudorabies virus US3 protein on nuclear membrane localization of the UL34 protein and virus egress from the nucleus. J Gen Virol 82, 2363–2371.
    [Google Scholar]
  8. Klupp, B. G., Fuchs, W., Granzow, H., Nixdorf, R. & Mettenleiter, T. C. ( 2002; ). Pseudorabies virus UL36 tegument protein physically interacts with the UL37 protein. J Virol 76, 3065–3071.[CrossRef]
    [Google Scholar]
  9. Klupp, B. G., Hengartner, C. J., Mettenleiter, T. C. & Enquist, L. W. ( 2004; ). Complete, annotated sequence of the pseudorabies virus genome. J Virol 78, 424–440.[CrossRef]
    [Google Scholar]
  10. Klupp, B. G., Bottcher, S., Granzow, H., Kopp, M. & Mettenleiter, T. C. ( 2005a; ). Complex formation between the UL16 and UL21 tegument proteins of pseudorabies virus. J Virol 79, 1510–1522.[CrossRef]
    [Google Scholar]
  11. Klupp, B. G., Granzow, H., Klopfleisch, R., Fuchs, W., Kopp, M., Lenk, M. & Mettenleiter, T. C. ( 2005b; ). Functional analysis of the pseudorabies virus UL51 protein. J Virol 79, 3831–3840.[CrossRef]
    [Google Scholar]
  12. Kopp, M., Granzow, H., Fuchs, W., Klupp, B. G., Mundt, E., Karger, A. & Mettenleiter, T. C. ( 2003; ). The pseudorabies virus UL11 protein is a virion component involved in secondary envelopment in the cytoplasm. J Virol 77, 5339–5351.[CrossRef]
    [Google Scholar]
  13. Kopp, M., Granzow, H., Fuchs, W., Klupp, B. & Mettenleiter, T. C. ( 2004; ). Simultaneous deletion of pseudorabies virus tegument protein UL11 and glycoprotein M severely impairs secondary envelopment. J Virol 78, 3024–3034.[CrossRef]
    [Google Scholar]
  14. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  15. Loomis, J. S., Courtney, R. J. & Wills, J. W. ( 2003; ). Binding partners for the UL11 tegument protein of herpes simplex virus type 1. J Virol 77, 11417–11424.[CrossRef]
    [Google Scholar]
  16. Michael, K., Klupp, B. G., Mettenleiter, T. C. & Karger, A. ( 2006; ). Composition of pseudorabies virus particles lacking tegument protein US3, UL47, or UL49 or envelope glycoprotein E. J Virol 80, 1332–1339.[CrossRef]
    [Google Scholar]
  17. Neuhoff, V., Arold, N., Taube, D. & Ehrhardt, W. ( 1988; ). Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9, 255–262.[CrossRef]
    [Google Scholar]
  18. Ong, S. E., Blagoev, B., Kratchmarova, I., Kristensen, D. B., Steen, H., Pandey, A. & Mann, M. ( 2002; ). Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol Cell Proteomics 1, 376–386.[CrossRef]
    [Google Scholar]
  19. Perkins, D. N., Pappin, D. J., Creasy, D. M. & Cottrell, J. S. ( 1999; ). Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20, 3551–3567.[CrossRef]
    [Google Scholar]
  20. Robbins, A. K., Weis, J. H., Enquist, L. W. & Watson, R. J. ( 1984; ). Construction of E. coli expression plasmid libraries: localization of a pseudorabies virus glycoprotein gene. J Mol Appl Genet 2, 485–496.
    [Google Scholar]
  21. Rosenfeld, J., Capdevielle, J., Guillemot, J. C. & Ferrara, P. ( 1992; ). In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis. Anal Biochem 203, 173–179.[CrossRef]
    [Google Scholar]
  22. Steven, A. C. & Spear, P. G. ( 1997; ). Herpesvirus capsid assembly and envelopment. In Structural Biology of Viruses, pp. 312–351. Edited by W. Chiu, R. M. Burnett & R. Garcea. New York: Oxford University Press.
  23. Towbin, H., Staehelin, T. & Gordon, J. ( 1979; ). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76, 4350–4354.[CrossRef]
    [Google Scholar]
  24. Zhou, Z. H., Chen, D. H., Jakana, J., Rixon, F. J. & Chiu, W. ( 1999; ). Visualization of tegument-capsid interactions and DNA in intact herpes simplex virus type 1 virions. J Virol 73, 3210–3218.
    [Google Scholar]
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