1887

Abstract

(BBTV) has a multi-component genome of circular, single-stranded DNA. BBTV replicates via a rolling-circle mechanism, probably involving sequence-specific interaction of the replication initiation protein (Rep) with iterated sequences (iterons) within the viral genome. Three putative iterons (designated F1, F2 and R), with the sequence GGGAC, have been identified in the intergenic region of each BBTV component. To investigate their role in replication, each of the iterons was mutated, singularly and in tandem, in a BBTV DNA-N 1.1mer and the ability of these molecules to be replicated by the BBTV ‘master’ Rep was evaluated in banana cells using transient biolistic assays. All iteron mutants were replicated less efficiently than the native DNA-N. Mutation of the F1 and R iterons caused a 42 and 62 % reduction in DNA-N replication, respectively, whereas mutation of the F2 and combined F1F2 iteron virtually abolished DNA-N replication.

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2006-11-01
2024-03-28
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References

  1. Argüello-Astorga G. R., Ruiz-Medrano R. 2001; An iteron-related domain is associated to Motif 1 in the replication proteins of geminiviruses: identification of potential interacting amino acid-base pairs by a comparative approach. Arch Virol 146:1465–1485 [CrossRef]
    [Google Scholar]
  2. Aronson M. N., Meyer A. D., Györgyey J., Katul L., Vetten H. J., Gronenborn B., Timchenko T. 2000; Clink, a nanovirus-encoded protein, binds both pRB and SKP1. J Virol 74:2967–2972 [CrossRef]
    [Google Scholar]
  3. Bell K. E., Dale J. L., Ha C. V., Vu M. T., Revill P. A. 2002; Characterisation of Rep-encoding components associated with banana bunchy top nanovirus in Vietnam. Arch Virol 147:695–707 [CrossRef]
    [Google Scholar]
  4. Burns T. M., Harding R. M., Dale J. L. 1995; The genome organization of banana bunchy top virus: analysis of six ssDNA components. J Gen Virol 76:1471–1482 [CrossRef]
    [Google Scholar]
  5. Chatterji A., Padidam M., Beachy R. N., Fauquet C. M. 1999; Identification of replication specificity determinants in two strains of tomato leaf curl virus from New Delhi. J Virol 73:5481–5489
    [Google Scholar]
  6. Chatterji A., Chatterji U., Beachy R., Fauquet C. 2000; Sequence parameters that determine specificity of binding of the replication-associated protein to its cognate site in two strains of Tomato leaf curl virus–New Delhi . Virology 273:341–350 [CrossRef]
    [Google Scholar]
  7. Choi I.-R., Stenger D. 1996; The strain-specific cis -acting element of beet curly top geminivirus DNA replication maps to the directly repeated motif of the ori . Virology 226:122–126 [CrossRef]
    [Google Scholar]
  8. Dugdale B., Beetham P. R., Becker D. K., Harding R. M., Dale J. L. 1998; Promoter activity associated with the intergenic regions of banana bunchy top virus DNA-1 to -6 in transgenic tobacco and banana cells. J Gen Virol 79:2301–2311
    [Google Scholar]
  9. Fontes E. P. B., Eagle P. A., Sipe P. S., Luckow V. A., Hanley-Bowdoin L. 1994a; Interaction between a geminivirus replication protein and origin DNA is essential for viral replication. J Biol Chem 269:8459–8465
    [Google Scholar]
  10. Fontes E. P. B., Gladfelter H. J., Schaffer R. L., Petty I. T. D., Hanley-Bowdoin L. 1994b; Geminivirus replication origins have a modular organization. Plant Cell 6:405–416 [CrossRef]
    [Google Scholar]
  11. Horser C. L. 2000; Characterisation of the putative satellite DNAs associated with banana bunchy top virus . PhD thesis Queensland University of Technology; Brisbane, Australia:
  12. Horser C. L., Harding R. M., Dale J. L. 2001; Banana bunchy top nanovirus DNA-1 encodes the ‘master’ replication initiation protein. J Gen Virol 82:459–464
    [Google Scholar]
  13. Mankertz A., Persson F., Mankertz J., Blaess G., Buhk H.-J. 1997; Mapping and characterization of the origin of DNA replication of porcine circovirus. J Virol 71:2562–2566
    [Google Scholar]
  14. Orozco B. M., Gladfelter H. J., Settlage S. B., Eagle P. A., Gentry R. N., Hanley-Bowdoin L. 1998; Multiple cis elements contribute to geminivirus origin function. Virology 242:346–356 [CrossRef]
    [Google Scholar]
  15. Sambrook J., Fritsch E., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Saunders K., Bedford I. D., Briddon R. W., Markham P. G., Wong S. M., Stanley J. 2000; A unique virus complex causes Ageratum yellow vein disease. Proc Natl Acad Sci U S A 97:6890–6895 [CrossRef]
    [Google Scholar]
  17. Steinfeldt T., Finsterbusch T., Mankertz A. 2001; Rep and Rep′ protein of Porcine circovirus type 1 bind to the origin of replication in vitro . Virology 291:152–160 [CrossRef]
    [Google Scholar]
  18. Stenger D. C., Revington G. N., Stevenson M. C., Bisaro D. M. 1991; Replicational release of geminivirus genomes from tandemly repeated copies: evidence for rolling-circle replication of a plant viral DNA. Proc Natl Acad Sci U S A 88:8029–8033 [CrossRef]
    [Google Scholar]
  19. Stewart C. N. Jr, Via L. E. 1993; A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Biotechniques 14:748–750
    [Google Scholar]
  20. Timchenko T., de Kouchkovsky F., Katul L., David C., Vetten H. J., Gronenborn B. 1999; A single Rep protein initiates replication of multiple genome components of faba bean necrotic yellows virus, a single-stranded DNA virus of plants. J Virol 73:10173–10182
    [Google Scholar]
  21. Timchenko T., Katul L., Sano Y., de Kouchkovsky F., Vetten H. J., Gronenborn B. 2000; The master Rep concept in nanovirus replication: identification of missing genome components and potential for natural genetic reassortment. Virology 274:189–195 [CrossRef]
    [Google Scholar]
  22. Vetten H. J., Chu P. W. G., Dale J. L. 7 other authors 2005; Nanoviridae . In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses pp  343–352 Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. San Diego: Elsevier Academic Press;
    [Google Scholar]
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