@article{mbs:/content/journal/jgv/10.1099/vir.0.82037-0, author = "Toan, Nguyen L. and Duechting, Anja and Kremsner, Peter G. and Song, Le H. and Ebinger, Martin and Aberle, Susanne and Binh, Vu Q. and Duy, Dinh Ng and Torresi, Joseph and Kandolf, Reinhard and Bock, C.-Thomas", title = "Phylogenetic analysis of human parvovirus B19, indicating two subgroups of genotype 1 in Vietnamese patients", journal= "Journal of General Virology", year = "2006", volume = "87", number = "10", pages = "2941-2949", doi = "https://doi.org/10.1099/vir.0.82037-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82037-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Recently, three distinct genotypes (1, 2 and 3) of human parvovirus B19 (B19) have been identified. However, the characteristics and distribution of B19 genotypes in Vietnam have not been investigated. Phylogenetic analysis using 49 subgenomic NS1/VP1u regions and two coding NS1–VP1/VP2 regions has been applied to investigate the prevalence of B19 genotypes in Vietnamese patients co-infected with Hepatitis B virus. Genetic analysis of the subgenomic NS1/VP1u region of B19 revealed that two genotypes of B19 were identified in these populations, with predominance of genotype 1 (47/49, 96 %) followed by genotype 2 (2/49, 4 %), but not genotype 3. Further, phylogenetic analysis of subgenomic B19 genomes revealed two major subgroups within genotype 1 (B19-1A and B19-1B) with an estimated nucleotide difference of >5 % between each subgroup, forming different branches. The mean percentage of amino acid variation between subgroup B19-1A and B19-1B was >2 % of the NS1, VP1 and VP2 proteins. Our results indicated that two of the three known genotypes of B19 were present in Vietnamese patients, with genotype 1 predominating, and that this genotype can be classified into at least two subgroups, B19-1A and B19-1B.", }