1887

Abstract

The subviral dodecahedral particle of adenovirus 3, which assembles spontaneously in insect cells expressing the viral penton base protein, shows promise as a vector for drug delivery. Its ability to gain cell entry has been demonstrated and recent structural analysis has outlined details of the interfaces between penton bases and the importance of proteolysis of the penton base N terminus for assembly, providing a basis for understanding particle assembly and stability. Here, work in manipulating the assembly status of the dodecahedron by changing buffer conditions and subsequent success in passively encapsidating a marker molecule is described. This represents an important stage towards development of the dodecahedral particle for use as a delivery vehicle capable of targeting therapeutic molecules to specific cell types.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.82025-0
2006-10-01
2024-10-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/10/2901.html?itemId=/content/journal/jgv/10.1099/vir.0.82025-0&mimeType=html&fmt=ahah

References

  1. Booy F. P., Newcomb W. W., Trus B. L., Brown J. C., Baker T. S., Steven A. C. 1991; Liquid-crystalline, phage-like packing of encapsidated DNA in herpes simplex virus. Cell 64:1007–1015 [CrossRef]
    [Google Scholar]
  2. Braun H., Boller K., Löwer J., Bertling W. M., Zimmer A. 1999; Oligonucleotide and plasmid DNA packaging into polyoma VP1 virus-like particles expressed in Escherichia coli . Biotechnol Appl Biochem 29:31–43
    [Google Scholar]
  3. Cheng N., Conway J. F., Watts N. R., Hainfeld J. F., Joshi V., Powell R. D., Stahl S. J., Wingfield P. E., Steven A. C. 1999; Tetrairidium, a four-atom cluster, is readily visible as a density label in three-dimensional cryo-EM maps of proteins at 10–25 Å resolution. J Struct Biol 127:169–176 [CrossRef]
    [Google Scholar]
  4. Fender P., Ruigrok R. W. H., Gout E., Buffet S., Chroboczek J. 1997; Adenovirus dodecahedron, a new vector for human gene transfer. Nat Biotechnol 15:52–56 [CrossRef]
    [Google Scholar]
  5. Fender P., Schoehn G., Foucaud-Gamen J., Gout E., Garcel A., Drouet E., Chroboczek J. 2003; Adenovirus dodecahedron allows large multimeric protein transduction in human cells. J Virol 77:4960–4964 [CrossRef]
    [Google Scholar]
  6. Fuschiotti P., Schoehn G., Fender P., Fabry C. M. S., Hewat E. A., Chroboczek J., Ruigrok R. W. H., Conway J. F. 2006; Structure of the dodecahedral penton particle from human adenovirus type 3. J Mol Biol 356:510–520 [CrossRef]
    [Google Scholar]
  7. Garcel A., Gout E., Timmins J., Chroboczek J., Fender P. 2006; Protein transduction into human cells by adenovirus dodecahedron using WW domains as universal adaptors. J Gene Med 8:524–531 [CrossRef]
    [Google Scholar]
  8. Greber U. F., Willetts M., Webster P., Helenius A. 1993; Stepwise dismantling of adenovirus 2 during entry into cells. Cell 75:477–486 [CrossRef]
    [Google Scholar]
  9. Mizuguchi H., Hayakawa T. 2004; Targeted adenovirus vectors. Hum Gene Ther 15:1034–1044 [CrossRef]
    [Google Scholar]
  10. Norrby E. 1966; The relationship between the soluble antigens and the virion of adenovirus type 3. II. Identification and characterization of an incomplete hemagglutinin. Virology 30:608–617 [CrossRef]
    [Google Scholar]
  11. Pettersson U., Höglund S. 1969; Structural proteins of adenoviruses. III. Purification and characterization of the adenovirus type 2 penton antigen. Virology 39:90–106 [CrossRef]
    [Google Scholar]
  12. Salunke D. M., Caspar D. L. D., Garcea R. L. 1986; Self-assembly of purified polyomavirus capsid protein VP1 . Cell 46:895–904 [CrossRef]
    [Google Scholar]
  13. Schoehn G., Fender P., Chroboczek J., Hewat E. A. 1996; Adenovirus 3 penton dodecahedron exhibits structural changes of the base on fibre binding. EMBO J 15:6841–6846
    [Google Scholar]
  14. Wickham T. J., Mathias P., Cheresh D. A., Nemerow G. R. 1993; Integrins α v β 3 and α v β 5 promote adenovirus internalization but not virus attachment. Cell 73:309–319 [CrossRef]
    [Google Scholar]
  15. Zubieta C., Schoehn G., Chroboczek J., Cusack S. 2005; The structure of the human adenovirus 2 penton. Mol Cell 17:121–135 [CrossRef]
    [Google Scholar]
/content/journal/jgv/10.1099/vir.0.82025-0
Loading
/content/journal/jgv/10.1099/vir.0.82025-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error