1887

Abstract

Adenoviral vectors can be used to generate potent humoral and cellular immune responses to transgene products. Use of adenoviral vectors based on non-human isolates may allow for their utilization in populations harbouring neutralizing antibodies to common human serotypes. A vector chimera was constructed using simian adenovirus 22 (a serotype belonging to the species ) and simian adenovirus 21 (a serotype belonging to the species ) expressing the Ebola (Zaire) virus glycoprotein (Ad C5/C1-ZGP). This chimeric adenovirus vector was used as a model to test its efficacy as a genetic vaccine and comparisons were made to a vector based on the commonly used human adenovirus C serotype 5 (Adhu5-ZGP). Ebola glycoprotein-specific T- and B-cell responses were measured in B10BR mice vaccinated with either Adhu5-ZGP or Ad C5/C1-ZGP vectors. Both vectors resulted in Ebola glycoprotein-specific gamma interferon-expressing T cells, although the Ad C5/C1-ZGP vector appeared to induce lower frequencies with kinetics slower than those elicited by the Adhu5-ZGP vector. The total immunoglobulin G response to Ebola glycoprotein was similar in sera from mice vaccinated with either vector. Two rhesus macaques vaccinated with the Ad C5/C1-ZGP vector were found to mount T-cell and antibody responses to the Ebola glycoprotein. It was found that a single administration of the chimeric Ad C5/C1-ZGP vector protected mice against a lethal challenge with a mouse-adapted strain of the Ebola (Zaire) virus.

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2006-09-01
2021-03-06
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References

  1. Abrahamsen K., Kong H. L., Mastrangeli A., Brough D., Lizonova A., Crystal G. R., Falck-Pedersen E. 1997; Construction of an adenovirus type 7a E1A-vector. J Virol 71:8946–8951
    [Google Scholar]
  2. Bray M., Davis K., Geisbert T., Schmaljohn C., Huggins J. 1998; A mouse model for evaluation of prophylaxis and therapy of Ebola hemorrhagic fever. J Infect Dis 178:651–661 [CrossRef]
    [Google Scholar]
  3. Farina S. F., Gao G. P., Xiang Z. Q., Rux J. J., Burnett R. M., Alvira M. R., Marsh J., Ertl H. C., Wilson J. M. 2001; Replication-defective vector based on a chimpanzee adenovirus. J Virol 75:11603–11613 [CrossRef]
    [Google Scholar]
  4. Gaggar A., Shayakhmetov D. M., Lieber A. 2003; CD46 is a cellular receptor for group B adenoviruses. Nat Med 9:1408–1412 [CrossRef]
    [Google Scholar]
  5. Gao W., Tamin A., Soloff A., D'Aiuto L., Nwanegbo E., Robbins P. D., Bellini W. J., Barratt-Boyes S., Gambotto A. 2003; Effects of a SARS-associated coronavirus vaccine in monkeys. Lancet 362:1895–1896 [CrossRef]
    [Google Scholar]
  6. Jooss K., Yang Y., Fisher K. J., Wilson J. M. 1998; Transduction of dendritic cells by DNA viral vectors directs the immune response to transgene products in muscle fibers. J Virol 72:4212–4223
    [Google Scholar]
  7. Kobinger G. P., Weiner D. J., Yu Q. C., Wilson J. M. 2001; Filovirus -pseudotyped lentiviral vector can efficiently and stably transduce airway epithelia in vivo . Nat Biotechnol 19:225–230 [CrossRef]
    [Google Scholar]
  8. Kremer E. J., Boutin S., Chillon M., Danos O. 2000; Canine adenovirus vectors: an alternative for adenovirus-mediated gene transfer. J Virol 74:505–512 [CrossRef]
    [Google Scholar]
  9. Mascola J. R., Sambor A., Beaudry K. & 12 other authors 2005; Neutralizing antibodies elicited by immunization of monkeys with DNA plasmids and recombinant adenoviral vectors expressing human immunodeficiency virus type 1 proteins. J Virol 79:771–779 [CrossRef]
    [Google Scholar]
  10. Mittal S. K., Middleton D. M., Tikoo S. K., Babiuk L. A. 1995; Pathogenesis and immunogenicity of bovine adenovirus type 3 in cotton rats ( Sigmodon hispidus ). Virology 213:131–139 [CrossRef]
    [Google Scholar]
  11. Noda T., Sagara H., Suzuki E., Takada A., Kida H., Kawaoka Y. 2002; Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP. J Virol 76:4855–4865 [CrossRef]
    [Google Scholar]
  12. Rao M., Matyas G. R., Grieder F., Anderson K., Jahrling P. B., Alving C. R. 1999; Cytotoxic T lymphocytes to Ebola Zaire virus are induced in mice by immunization with liposomes containing lipid A. Vaccine 17:2991–2998 [CrossRef]
    [Google Scholar]
  13. Rowe W. P., Hartley J. W., Huebner R. J. 1956; Additional serotypes of the APC virus group. Proc Soc Exp Biol Med 91:260–262 [CrossRef]
    [Google Scholar]
  14. Rowe W. P., Hartley J. W., Huebner R. J. 1958; Serotype composition of the adenovirus group. Proc Soc Exp Biol Med 97:465–470 [CrossRef]
    [Google Scholar]
  15. Roy S., Gao G., Clawson D. S., Vandenberghe L. H., Farina S. F., Wilson J. M. 2004a; Complete nucleotide sequences and genome organization of four chimpanzee adenoviruses. Virology 324:361–372 [CrossRef]
    [Google Scholar]
  16. Roy S., Gao G., Lu Y., Zhou X., Lock M., Calcedo R., Wilson J. M. 2004b; Characterization of a family of chimpanzee adenoviruses and development of molecular clones for gene transfer vectors. Hum Gene Ther 15:519–530 [CrossRef]
    [Google Scholar]
  17. Santra S., Seaman M. S., Xu L. & 13 other authors 2005; Replication-defective adenovirus serotype 5 vectors elicit durable cellular and humoral immune responses in nonhuman primates. J Virol 79:6516–6522 [CrossRef]
    [Google Scholar]
  18. Segerman A., Atkinson J. P., Marttila M., Dennerquist V., Wadell G., Arnberg N. 2003; Adenovirus type 11 uses CD46 as a cellular receptor. J Virol 77:9183–9191 [CrossRef]
    [Google Scholar]
  19. Shiver J. W., Fu T. M., Chen L. 49 other authors 2002; Replication-incompetent adenoviral vaccine vector elicits effective anti-immunodeficiency-virus immunity. Nature 415:331–335 [CrossRef]
    [Google Scholar]
  20. Sirena D., Lilienfeld B., Eisenhut M. & 8 other authors 2004; The human membrane cofactor CD46 is a receptor for species B adenovirus serotype 3. J Virol 78:4454–4462 [CrossRef]
    [Google Scholar]
  21. Vogels R., Zuijdgeest D., van Rijnsoever R. & 20 other authors 2003; Replication-deficient human adenovirus type 35 vectors for gene transfer and vaccination: efficient human cell infection and bypass of preexisting adenovirus immunity. J Virol 77:8263–8271 [CrossRef]
    [Google Scholar]
  22. Wigand R., Mauss M., Adrian T. 1989; Chimpanzee adenoviruses are related to four subgenera of human adenoviruses. Intervirology 30:1–9 [CrossRef]
    [Google Scholar]
  23. Willimzik H. F., Kalter S. S., Lester T. L., Wigand R. 1981; Immunological relationship among adenoviruses of humans, simians, and nonprimates as determined by the neutralization test. Intervirology 15:28–36 [CrossRef]
    [Google Scholar]
  24. Xu Z. Z., Hyatt A., Boyle D. B., Both G. W. 1997; Construction of ovine adenovirus recombinants by gene insertion or deletion of related terminal region sequences. Virology 230:62–71 [CrossRef]
    [Google Scholar]
  25. Yang Y., Ertl H. C., Wilson J. M. 1994; MHC class I-restricted cytotoxic T lymphocytes to viral antigens destroy hepatocytes in mice infected with E1-deleted recombinant adenoviruses. Immunity 1:433–442 [CrossRef]
    [Google Scholar]
  26. Yang Y., Li Q., Ertl H. C., Wilson J. M. 1995; Cellular and humoral immune responses to viral antigens create barriers to lung-directed gene therapy with recombinant adenoviruses. J Virol 69:2004–2015
    [Google Scholar]
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