1887

Abstract

This study analysed the transport behaviour of the glycoprotein E2 of (BVDV) expressed from recombinant vesicular stomatitis virus (rVSV). E2 protein was found to be retained at an intracellular compartment. A chimeric protein containing the membrane anchor and cytoplasmic tail of the VSV G protein, E2–G(MT), was transported to the cell surface. Only the latter protein was incorporated into rVSV particles in significant amounts. A soluble form of E2 lacking the membrane anchor, E2(MTdel), appeared to be affected in conformational stability. In contrast to both membrane-anchored forms of E2, expression of the soluble form was detectable only by immunofluorescence microscopy but not by Western blotting. These results are in agreement with reports of intracellular retention of the E2 protein due to a retention signal in the membrane anchor. However, in another analysis of E2 expressed from rVSV, E2 protein was reported to be transported to the cell surface and incorporated into VSV particles [ Grigera, P. R., Marzocca, M. P., Capozzo, A. V. E., Buonocore, L., Donis, R. O. & Rose, J. K. (2000). , 3–15 ]. Reasons for these contradictory results are discussed.

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2007-01-01
2024-12-04
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References

  1. Allison S. L., Stiasny K., Stadler K., Mandl C. W., Heinz F. X. 1999; Mapping of functional elements in the stem–anchor region of tick-borne encephalitis virus envelope protein E. J Virol 73:5605–5612
    [Google Scholar]
  2. Baker J. C. 1995; The clinical manifestations of bovine viral diarrhea infection. Vet Clin North Am Food Anim Pract 11:425–445
    [Google Scholar]
  3. Bolin S. R., Ridpath J. F. 1996; Glycoprotein E2 of bovine viral diarrhea virus expressed in insect cells provides calves limited protection from systemic infection and disease. Arch Virol 141:1463–1477 [CrossRef]
    [Google Scholar]
  4. Bolin S., Moennig V., Kelso Gourley N. E., Ridpath J. F. 1988; Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhoea virus into groups. Brief report. Arch Virol 99:117–123 [CrossRef]
    [Google Scholar]
  5. Buchholz U. J., Finke S., Conzelmann K.-K. 1999; Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J Virol 73:251–259
    [Google Scholar]
  6. Cocquerel L., Meunier J. C., Pillez A., Wychowski C., Dubuisson J. 1998; A retention signal necessary and sufficient for endoplasmic reticulum localization maps to the transmembrane domain of hepatitis C virus glycoprotein E2. J Virol 72:2183–2191
    [Google Scholar]
  7. Ezelle H. J., Markovic D., Barber G. N. 2002; Generation of hepatitis C virus-like particles by use of a recombinant vesicular stomatitis virus vector. J Virol 76:12325–12334 [CrossRef]
    [Google Scholar]
  8. Fehr T., Bachmann M. F., Bluethmann H., Kikutani H., Hengartner H., Zinkernagel R. M. 1996; T-independent activation of B cells by vesicular stomatitis virus: no evidence for the need of a second signal. Cell Immunol 168:184–192 [CrossRef]
    [Google Scholar]
  9. Garbutt M., Liebscher R., Wahl-Jensen V., Jones S., Möller P., Wagner R., Volchkov V., Klenk H.-D., Feldmann H., Ströher U. 2004; Properties of replication-competent vesicular stomatitis virus vectors expressing glycoproteins of filoviruses and arenaviruses. J Virol 78:5458–5465 [CrossRef]
    [Google Scholar]
  10. Gray E. W., Nettleton P. F. 1987; The ultrastructure of cell cultures infected with border disease and bovine viral diarrhoea viruses. J Gen Virol 68:2339–2346 [CrossRef]
    [Google Scholar]
  11. Greiser-Wilke I., Dittmar K. E., Liess B., Moennig V. 1991; Immunofluorescence studies of biotype-specific expression of bovine viral diarrhoea virus epitopes in infected cells. J Gen Virol 72:2015–2019 [CrossRef]
    [Google Scholar]
  12. Grigera P. R., Marzocca M. P., Capozzo A. V. E., Buonocore L., Donis R. O., Rose J. K. 2000; Presence of bovine viral diarrhea virus (BVDV) E2 glycoprotein in VSV recombinant particles and induction of neutralizing BVDV antibodies in mice. Virus Res 69:3–15 [CrossRef]
    [Google Scholar]
  13. Grummer B., Beer M., Liebler-Tenorio E., Greiser-Wilke I. 2001; Localization of viral proteins in cells infected with bovine viral diarrhoea virus. J Gen Virol 82:2597–2605
    [Google Scholar]
  14. Haglund K., Formann J., Kräusslich H. G., Rose J. K. 2000; Expression of human immunodeficiency virus type 1 Gag protein precursor and envelope proteins from a vesicular stomatitis virus recombinant: high level production of virus-like particles containing HIV envelope. Virology 268:112–121 [CrossRef]
    [Google Scholar]
  15. Haglund K., Leiner I., Kerksiek K., Buonocore L., Pamer E., Rose J. K. 2002; High-level primary CD8+ T-cell response to human immunodeficiency virus type 1 Gag and Env generated by vaccination with recombinant vesicular stomatitis viruses. J Virol 76:2730–2738 [CrossRef]
    [Google Scholar]
  16. Harpin S., Talbot B., Mbikay M., Elazhary Y. 1997; Immune response to vaccination with DNA encoding the bovine viral diarrhea virus major glycoprotein gp 43 (E2). FEMS Microbiol Lett 146:229–234 [CrossRef]
    [Google Scholar]
  17. Harpin S., Hurley D. J., Mbikay M., Talbot B., Elazhary Y. 1999; Vaccination of cattle with a DNA plasmid encoding the bovine viral diarrhea virus major glycoprotein E2. J Gen Virol 80:3137–3144
    [Google Scholar]
  18. Johnson J. E., Schnell M. J., Buonocore L., Rose J. K. 1997; Specific targeting to CD4+ cells of recombinant vesicular stomatitis viruses encoding human immunodeficiency virus envelope proteins. J Virol 71:5060–5068
    [Google Scholar]
  19. Kahn S. J., Schnell M. J., Buonocore L., Rose J. K. 1997; Recombinant vesicular stomatitis virus expressing respiratory syncytial virus (RSV) glycoproteins: RSV fusion protein can mediate infection and cell fusion. Virology 254:81–91
    [Google Scholar]
  20. Köhl W., Zimmer G., Greiser-Wilke I., Haas L., Moennig V., Herrler G. 2004; The surface glycoprotein E2 of bovine viral diarrhoea virus contains an intracellular localization signal. J Gen Virol 85:1101–1111 [CrossRef]
    [Google Scholar]
  21. Krempl C., Herrler G. 2001; Sialic acid binding activity of transmissible gastroenteritis coronavirus affects sedimentation behavior of virions and solubilized glycoproteins. J Virol 75:844–849 [CrossRef]
    [Google Scholar]
  22. Kretzschmar E., Buonocore L., Schnell M. J., Rose J. K. 1997; High-efficiency incorporation of functional influenza virus glycoproteins into recombinant vesicular stomatitis virus. J Virol 71:5982–5989
    [Google Scholar]
  23. Kyhse-Anderson J. 1984; Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods 10:203–209 [CrossRef]
    [Google Scholar]
  24. Lawson N. D., Stillmann E. A., Whitt M. A., Rose J. K. 1995; Recombinant vesicular stomatitis viruses from DNA. Proc Natl Acad Sci U S A 92:4477–4481 [CrossRef]
    [Google Scholar]
  25. Meyers G., Thiel H.-J. 1996; Molecular characterization of pestiviruses. Adv Virus Res 47:53–118
    [Google Scholar]
  26. Nobiron I., Thompson I., Brownlie J., Collins M. E. 2001; Cytokine adjuvancy of BVDV DNA vaccine enhances both humoral and cellular immune responses in mice. Vaccine 19:4226–4235 [CrossRef]
    [Google Scholar]
  27. Nobiron I., Thompson I., Brownlie J., Collins M. E. 2003; DNA vaccination against bovine viral diarrhea virus induces humoral and cellular immune responses in cattle with evidence for protection against viral challenge. Vaccine 21:2082–2092 [CrossRef]
    [Google Scholar]
  28. Orban S., Liess B., Hafez S. M., Frey H.-R., Blindow H., Sasse-Patzer B. 1983; Studies on transplacental transmissibility of bovine virus diarrhoea (BVD) vaccine virus. I. Inoculation of pregnant cows 15 to 90 days before parturition (190th to 265th day of gestation). Zentralbl Veterinarmed B 30:619–634
    [Google Scholar]
  29. Roberts A., Kretzschmar E., Perkins A. S., Formann J., Price R., Buonocore L., Kawaoka Y., Rose J. K. 1998; Vaccination with a recombinant vesicular stomatitis virus expressing an influenza virus hemagglutinin provides complete protection from influenza virus challenge. J Virol 72:4704–4711
    [Google Scholar]
  30. Rose N. F., Roberts A., Buonocore L., Rose J. K. 2000; Glycoprotein exchange vectors based on vesicular stomatitis virus allow effective boosting and generation of neutralizing antibodies to a primary isolate of human immunodeficiency virus type 1. J Virol 74:10903–10910 [CrossRef]
    [Google Scholar]
  31. Rose N. F., Marx P. A., Luckay A., Nixon D. F., Moretto W. J., Donahoe S. M., Montefirio D., Roberts A., Buonocore L., Rose J. K. 2001; An effective AIDS vaccine based on live attenuated vesicular stomatitis virus recombinants. Cell 106:539–549 [CrossRef]
    [Google Scholar]
  32. Rümenapf T., Unger G., Strauss J. H., Thiel H.-J. 1993; Processing of the envelope glycoproteins of pestiviruses. J Virol 67:3288–3294
    [Google Scholar]
  33. Schlereth B., Rose J. K., Buonocore L., ter Meulen V., Niewiesk S. 2000; Successful vaccine-induced seroconversion by single-dose immunization in the presence of measles virus-specific maternal antibodies. J Virol 74:4652–4657 [CrossRef]
    [Google Scholar]
  34. Schnell M. J., Buonocore L., Whitt M. A., Rose J. K. 1996a; The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. J Virol 70:2318–2323
    [Google Scholar]
  35. Schnell M. J., Buonocore L., Kretzschmar E., Johnson E., Rose J. K. 1996b; Foreign glycoproteins expressed from recombinant vesicular stomatitis viruses are incorporated efficiently into virus particles. Proc Natl Acad Sci U S A 93:11359–11365 [CrossRef]
    [Google Scholar]
  36. Schwegmann-Wessels C., Al-Falah M., Escors D., Wang Z., Zimmer G., Deng H., Enjuanes L., Naim H. Y., Herrler G. 2004; A novel sorting signal for intracellular localization is present in the S protein of a porcine coronavirus but absent from severe acute respiratory syndrome-associated coronavirus. J Biol Chem 279:43661–43666 [CrossRef]
    [Google Scholar]
  37. Sutter G., Ohlmann M., Erfle V. 1995; Non-replicating vaccinia vector efficiently expresses bacteriophage T7 RNA polymerase. FEBS Lett 371:9–12 [CrossRef]
    [Google Scholar]
  38. Tautz N., Thiel H.-J., Dubovi E. J., Meyers G. 1994; Pathogenesis of mucosal disease: a cytopathic pestivirus generated by an internal deletion. J Virol 68:3289–3297
    [Google Scholar]
  39. Thiel H.-J., Stark R., Weiland E., Rümenapf T., Meyers G. 1991; Hog cholera virus: molecular composition of virions from a pestivirus. J Virol 65:4705–4712
    [Google Scholar]
  40. Wagner R. R., Rose J. K. 1996; Rhabdoviridae: the viruses and their replication. In Fields Virology pp  1121–1136 Edited by Fields B. N., Knipe D. M. New York, NY: Lippincott–Raven;
    [Google Scholar]
  41. Wang Z., Nie Y., Wang P., Ding M., Deng H. 2004; Characterization of classical swine fever virus entry by using pseudotyped viruses: E1 and E2 are sufficient to mediate viral entry. Virology 330:332–341 [CrossRef]
    [Google Scholar]
  42. Weiland F., Weiland E., Unger G., Saalmuller A., Thiel H.-J. 1999; Localization of pestiviral envelope proteins Erns and E2 at the cell surface and on isolated particles. J Gen Virol 80:1157–1165
    [Google Scholar]
  43. Wengler G., Bradley D. W., Collett M. S., Heinz F. X., Schlesinger R. W., Strauss J. H. 1995; Flaviviridae . In Virus Taxonomy. Sixth Report of the International Commitee on Taxonomy of Viruses pp  415–427 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. New York: Springer;
    [Google Scholar]
  44. Zhou Y., König M., Hobom G., Neumeier E. 1998; Membrane-anchored incorporation of a foreign protein in recombinant influenza virions. Virology 246:83–94 [CrossRef]
    [Google Scholar]
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