1887

Abstract

Vaccines used in control programmes of (BoHV-1) utilize highly attenuated BoHV-1 strains marked by a deletion of the glycoprotein E (gE) gene. Since BoHV-1 recombinants are obtained at high frequency in experimentally coinfected cattle, the consequences of recombination on the virulence of gE-negative BoHV-1 were investigated. Thus, gE-negative BoHV-1 recombinants were generated from several virulent BoHV-1 and one mutant BoHV-1 deleted in the gC and gE genes. Four gE-negative recombinants were tested in the natural host. All the recombinants were more virulent than the gE-negative BoHV-1 vaccine and the gC- and gE-negative parental BoHV-1. The gE-negative recombinant isolated from a BoHV-1 field strain induced the highest severe clinical score. Latency and reactivation studies showed that three of the recombinants were reexcreted. Recombination can therefore restore virulence of gE-negative BoHV-1 by introducing the gE deletion into a different virulence background.

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2006-08-01
2019-11-19
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Supplements

vol. , part 8, pp. 2149 - 2154

Detection of latent BoHV-1 DNA in trigeminal ganglion

Description of the BoHV-1 viruses used in this study

Endoscope scores of two calves per group obtained by investigating the next criteria: the number, size and depth of the lesions; presence of ulcers and degree of hyperaemia.

Southern blot analysis

PCR results on trigeminal ganglions of the mock-infected and infected calves [Single PDF file](350 KB)



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