1887

Abstract

Previous studies have identified virus proteins that traffic to mitochondria and may affect mitochondrial function. Here, it is reported that (HHV-1, herpes simplex virus 1) and influenza virus reduced mitochondrial respiration, whilst , cytomegalovirus, coxsackievirus B4 and did not. The inhibition of total cellular respiration was caused by a block in the mitochondrial electron-transport chain. This effect occurred during β-phase protein synthesis and the inhibition of mitochondrial respiration could be reproduced by ectopic expression of the -phase protein U3. An HHV-1 mutant lacking this protein failed to inhibit oxygen consumption in infected cells relative to controls. It was concluded that U3 was mediating the suppression of mitochondrial respiration following HHV-1 infection. The integrity of the electron-transport chain in HHV-1-infected cells was analysed further and the site of the block in electron transport was located between complexes II and III, a site previously shown to be affected by .

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2006-08-01
2019-12-11
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