1887

Abstract

The human polyomavirus JC (JCV) typically infects glial cells and is the aetiological agent of progressive multifocal leukoencephalopathy (PML), which occurs in immunosuppressed individuals. The full-length sequence of a granule cell neuron-tropic JCV variant, JCV, associated with lytic infection of granule cell neurons and cerebellar atrophy in a human immunodeficiency virus-infected patient with PML was determined and compared with the sequence of the JCV isolate from the classic PML lesions present in the hemispheric white matter of the same individual (JCV). A unique deletion was found in the C terminus of the VP1 gene of JCV, which encodes the major capsid protein, resulting in a frame shift and a total change of the C-terminal amino acid sequence of this protein. This deletion was not present in JCV, suggesting that this mutation may be instrumental in facilitating entry or replication of JCV into granule cell neurons.

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2006-09-01
2019-11-15
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References

  1. Agostini, H. T. & Stoner, G. L. ( 1995; ). Amplification of the complete polyomavirus JC genome from brain, cerebrospinal fluid and urine using pre-PCR restriction enzyme digestion. J Neurovirol 1, 316–320.[CrossRef]
    [Google Scholar]
  2. Agostini, H. T., Ryschkewitsch, C. F., Brubaker, G. R., Shao, J. & Stoner, G. I. ( 1997; ). Five complete genomes of JC virus type 3 from Africans and African Americans. Arch Virol 142, 637–655.[CrossRef]
    [Google Scholar]
  3. Bauer, P. H., Bronson, R. T., Fung, S. C., Freund, R., Stehle, T., Harrison, S. C. & Benjamin, T. L. ( 1995; ). Genetic and structural analysis of a virulence determinant in polyomavirus VP1. J Virol 69, 7925–7931.
    [Google Scholar]
  4. Du Pasquier, R. A., Corey, S., Margolin, D. H. & 7 other authors ( 2003; ). Productive infection of cerebellar granule cell neurons by JC virus in an HIV+ individual. Neurology 61, 775–782.[CrossRef]
    [Google Scholar]
  5. Du Pasquier, R. A., Stein, M. C., Lima, M. A., Dang, X., Jean-Jacques, J., Zheng, Y., Letvin, N. L. & Koralnik, I. J. ( 2006; ). JC virus induces a vigorous CD8+ cytotoxic T cell response in multiple sclerosis patients. J Neuroimmunol (in press). doi:10.1016/j.jneuroim.2006.04.003
    [Google Scholar]
  6. Elphick, G. F., Querbes, W., Jordan, J. A. & 9 other authors ( 2004; ). The human polyomavirus, JCV, uses serotonin receptors to infect cells. Science 306, 1380–1383.[CrossRef]
    [Google Scholar]
  7. Freund, R., Garcea, R. L., Sahli, R. & Benjamin, T. L. ( 1991; ). A single-amino-acid substitution in polyomavirus VP1 correlates with plaque size and hemagglutination behavior. J Virol 65, 350–355.
    [Google Scholar]
  8. Gahaffar, H., Hicks, R. J. & Moral, L. A. ( 2005; ). Changes in the internal granular cell layer in progressive multifocal leukoencephalopathy in 3 patients with AIDS. In United States and Canadian Academy of Pathology Meeting, 26 February–4 March 2005, San Antonio, TX, USA.
  9. Kim, J. Y. H., Koralnik, I. J., LeFave, M., Segal, R. A., Pfister, L.-A. & Pomeroy, S. L. ( 2002; ). Medulloblastomas and primitive neuroectodermal tumors rarely contain polyomavirus DNA sequences. Neuro-oncol 4, 165–170.
    [Google Scholar]
  10. Kim, J. Y. H., Nelson, A. L., Algon, S. A., Graves, O., Sturla, L. M., Goumnerova, L. C., Rowitch, D. H., Segal, R. A. & Pomeroy, S. L. ( 2003; ). Medulloblastoma tumorigenesis diverges from cerebellar granule cell differentiation in patched heterozygous mice. Dev Biol 263, 50–66.[CrossRef]
    [Google Scholar]
  11. Komagome, R., Sawa, H., Suzuki, T., Suzuki, Y., Tanaka, S., Atwood, W. J. & Nagashima, K. ( 2002; ). Oligosaccharides as receptors for JC virus. J Virol 76, 12992–13000.[CrossRef]
    [Google Scholar]
  12. Koralnik, I. J., Schmitz, J. E., Lifton, M. A., Forman, M. A. & Letvin, N. L. ( 1999; ). Detection of JC virus DNA in peripheral blood cell subpopulations of HIV-1-infected individuals. J Neurovirol 5, 430–435.[CrossRef]
    [Google Scholar]
  13. Koralnik, I. J., Wüthrich, C., Dang, X., Rottnek, M., Gurtman, A., Simpson, D. & Morgello, S. ( 2005; ). JC virus granule cell neuronopathy: a novel clinical syndrome distinct from progressive multifocal leukoencephalopathy. Ann Neurol 57, 576–580.[CrossRef]
    [Google Scholar]
  14. Krynska, B., Del Valle, L., Croul, S., Gordon, J., Katsetos, C. D., Carbone, M., Giordano, A. & Khalili, K. ( 1999; ). Detection of human neurotropic JC virus DNA sequence and expression of the viral oncogenic protein in pediatric medulloblastomas. Proc Natl Acad Sci U S A 96, 11519–11524.[CrossRef]
    [Google Scholar]
  15. Loeber, G. & Dorries, K. ( 1988; ). DNA rearrangements in organ-specific variants of polyomavirus JC strain GS. J Virol 62, 1730–1735.
    [Google Scholar]
  16. Major, E. O., Miller, A. E., Mourrain, P., Traub, R. G., de Widt, E. & Sever, J. ( 1985; ). Establishment of a line of human fetal glial cells that supports JC virus multiplication. Proc Natl Acad Sci U S A 82, 1257–1261.[CrossRef]
    [Google Scholar]
  17. Padgett, B. L., Walker, D. L., ZuRhein, G. M., Eckroade, R. J. & Dessel, B. H. ( 1971; ). Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet i, 1257–1260.
    [Google Scholar]
  18. Pasqualetti, M., Ori, M., Nardi, I., Castagna, M., Cassano, G. B. & Marazziti, D. ( 1998; ). Distribution of the 5-HT5A serotonin receptor mRNA in the human brain. Brain Res Mol Brain Res 56, 1–8.[CrossRef]
    [Google Scholar]
  19. Pfister, L.-A., Letvin, N. L. & Koralnik, I. J. ( 2001; ). JC virus regulatory region tandem repeats in plasma and central nervous system isolates correlate with poor clinical outcome in patients with progressive multifocal leukoencephalopathy. J Virol 75, 5672–5676.[CrossRef]
    [Google Scholar]
  20. Pho, M. T., Ashok, A. & Atwood, W. J. ( 2000; ). JC virus enters human glial cells by clathrin-dependent receptor-mediated endocytosis. J Virol 74, 2288–2292.[CrossRef]
    [Google Scholar]
  21. Raj, G. V. & Khalili, K. ( 1995; ). Transcriptional regulation: lessons from the human neurotropic polyomavirus, JCV. Virology 213, 283–291.[CrossRef]
    [Google Scholar]
  22. Ressetar, H. G., Walker, D. L., Webster, H. D., Braun, D. G. & Stoner, G. L. ( 1990; ). Immunolabeling of JC virus large T antigen in neonatal hamster brain before tumor formation. Lab Invest 62, 287–296.
    [Google Scholar]
  23. Stehle, T., Yan, Y., Benjamin, T. L. & Harrison, S. C. ( 1994; ). Structure of murine polyomavirus complexed with an oligosaccharide receptor fragment. Nature 369, 160–163.[CrossRef]
    [Google Scholar]
  24. Stehle, T., Gamblin, S. J., Yan, Y. & Harrison, S. C. ( 1996; ). The structure of simian virus 40 refined at 3.1 Å resolution. Structure 4, 165–182.[CrossRef]
    [Google Scholar]
  25. Sumner, C., Shinohara, T., Durham, L., Traub, R., Major, E. O. & Amemiya, K. ( 1996; ). Expression of multiple classes of the nuclear factor-1 family in the developing human brain: differential expression of two classes of NF-1 genes. J Neurovirol 2, 87–100.[CrossRef]
    [Google Scholar]
  26. Tyler, K. L. ( 2003; ). The uninvited guest: JC virus infection of neurons in PML. Neurology 61, 0734–735.[CrossRef]
    [Google Scholar]
  27. Weggen, S., Bayer, T. A., von Deimling, A., Reifenberger, G., von Schweinitz, D., Wiestler, O. D. & Pietsch, T. ( 2000; ). Low frequency of SV40, JC and BK polyomavirus sequences in human medulloblastomas, meningiomas and ependymomas. Brain Pathol 10, 85–92.
    [Google Scholar]
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