1887

Abstract

The and genes of encode homologous proteins that are required for tobamovirus multiplication. Although the genome encodes another -like gene, , the tobamovirus coat protein (CP) does not accumulate to a detectable level in the double mutant. Here, double and triple mutants of , and were generated to investigate whether THH1 functions to support tobamovirus multiplication. In the double mutant, the tobamovirus CP accumulated to a level that was detectable, but lower than that in the single mutant. In double-mutant lines overexpressing , the tobamovirus CP accumulated to a level similar to that observed in wild-type plants. These results suggest that supports tobamovirus multiplication, but to a lesser extent than and . The expression level of is lower than that of and , which might explain the smaller contribution of to tobamovirus multiplication.

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2006-08-01
2019-11-14
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References

  1. Ahlquist, P., Noueiry, A. O., Lee, W.-M., Kushner, D. B. & Dye, B. T. ( 2003; ). Host factors in positive-strand RNA virus genome replication. J Virol 77, 8181–8186.[CrossRef]
    [Google Scholar]
  2. Asano, M., Satoh, R., Mochizuki, A. & 7 other authors ( 2005; ). Tobamovirus-resistant tobacco generated by RNA interference directed against host genes. FEBS Lett 579, 4479–4484.[CrossRef]
    [Google Scholar]
  3. Buck, K. W. ( 1999; ). Replication of tobacco mosaic virus RNA. Philos Trans R Soc Lond B Biol Sci 354, 613–627.[CrossRef]
    [Google Scholar]
  4. Clough, S. J. & Bent, A. F. ( 1998; ). Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16, 735–743.[CrossRef]
    [Google Scholar]
  5. Fujisaki, K., Hagihara, F., Azukawa, Y., Kaido, M., Okuno, T. & Mise, K. ( 2004; ). Identification and characterization of the SSB1 locus involved in symptom development by Spring beauty latent virus infection in Arabidopsis thaliana. Mol Plant Microbe Interact 17, 967–975.[CrossRef]
    [Google Scholar]
  6. Hagiwara, Y., Komoda, K., Yamanaka, T., Tamai, A., Meshi, T., Funada, R., Tsuchiya, T., Naito, S. & Ishikawa, M. ( 2003; ). Subcellular localization of host and viral proteins associated with tobamovirus RNA replication. EMBO J 22, 344–353.[CrossRef]
    [Google Scholar]
  7. Ishikawa, M., Obata, F., Kumagai, T. & Ohno, T. ( 1991; ). Isolation of mutants of Arabidopsis thaliana in which accumulation of tobacco mosaic virus coat protein is reduced to low levels. Mol Gen Genet 230, 33–38.[CrossRef]
    [Google Scholar]
  8. Ishikawa, M., Naito, S. & Ohno, T. ( 1993; ). Effects of the tom1 mutation of Arabidopsis thaliana on the multiplication of tobacco mosaic virus RNA in protoplasts. J Virol 67, 5328–5338.
    [Google Scholar]
  9. Neff, M. M., Neff, J. D., Chory, J. & Pepper, A. E. ( 1998; ). dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics. Plant J 14, 387–392.[CrossRef]
    [Google Scholar]
  10. Noueiry, A. O. & Ahlquist, P. ( 2003; ). Brome mosaic virus RNA replication: revealing the role of the host in RNA virus replication. Annu Rev Phytopathol 41, 77–98.[CrossRef]
    [Google Scholar]
  11. Rosso, M. G., Li, Y., Strizhov, N., Reiss, B., Dekker, K. & Weisshaar, B. ( 2003; ). An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics. Plant Mol Biol 53, 247–259.[CrossRef]
    [Google Scholar]
  12. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  13. Tsujimoto, Y., Numaga, T., Ohshima, K., Yano, M.-A., Ohsawa, R., Goto, D. B., Naito, S. & Ishikawa, M. ( 2003; ). Arabidopsis TOBAMOVIRUS MULTIPLICATION (TOM) 2 locus encodes a transmembrane protein that interacts with TOM1. EMBO J 22, 335–343.[CrossRef]
    [Google Scholar]
  14. Yamanaka, T., Komatani, H., Meshi, T., Naito, S., Ishikawa, M. & Ohno, T. ( 1998; ). Complete nucleotide sequence of the genomic RNA of tobacco mosaic virus strain Cg. Virus Genes 16, 173–176.[CrossRef]
    [Google Scholar]
  15. Yamanaka, T., Ohta, T., Takahashi, M., Meshi, T., Schmidt, R., Dean, C., Naito, S. & Ishikawa, M. ( 2000; ). TOM1, an Arabidopsis gene required for efficient multiplication of a tobamovirus, encodes a putative transmembrane protein. Proc Natl Acad Sci U S A 97, 10107–10112.[CrossRef]
    [Google Scholar]
  16. Yamanaka, T., Imai, T., Satoh, R. & 7 other authors ( 2002; ). Complete inhibition of tobamovirus multiplication by simultaneous mutations in two homologous host genes. J Virol 76, 2491–2497.[CrossRef]
    [Google Scholar]
  17. Yoshii, M., Nishikiori, M., Tomita, K., Yoshioka, N., Kozuka, R., Naito, S. & Ishikawa, M. ( 2004; ). The Arabidopsis cucumovirus multiplication 1 and 2 loci encode translation initiation factors 4E and 4G. J Virol 78, 6102–6111.[CrossRef]
    [Google Scholar]
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