1887

Abstract

Infection of (cynomolgus monkey) with chimeric simian/human immunodeficiency virus (SHIV) provides a valuable experimental animal model of AIDS and is widely used for the development of human immunodeficiency virus vaccine strategies. In these settings, analysis of CD8 T-cell responses during infection represents one of the key parameters for monitoring the evaluation of containment of virus replication. The generation of Gag-specific CD8 T cells was reported previously from a cynomolgus monkey infected with SHIV89.6P by taking advantage of a B-lymphoblastoid cell line transduced with a retroviral vector expressing simian immunodeficiency virus (SIV) Gag. Here, it was shown that these cytotoxic T lymphocytes (CTLs) demonstrated specificity for a single 9 aa peptide (NCVGDHQAA) spanning aa 192–200 of the SIVmac239 p55 protein. Furthermore, a positive response was found against the same epitope in one of six other SHIV-infected monkeys. This newly identified SIV Gag CTL epitope in SHIV-infected cynomolgus monkeys will be a useful tool for monitoring and evaluating Gag-specific immune responses during vaccination and infection in the cynomolgus monkey model of AIDS.

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2006-11-01
2024-12-07
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References

  1. Appay V., Dunbar P. R., Callan M. & 18 other authors 2002; Memory CD8+ T cells vary in differentiation phenotype in different persistent virus infections. Nat Med 8:379–385 [CrossRef]
    [Google Scholar]
  2. Barouch D. H., Santra S., Schmitz J. E. & 26 other authors 2000; Control of viremia and prevention of clinical AIDS in rhesus monkeys by cytokine-augmented DNA vaccination. Science 290:486–492 [CrossRef]
    [Google Scholar]
  3. Barouch D. H., Kunstman J., Kuroda M. J. & 11 other authors 2002; Eventual AIDS vaccine failure in a rhesus monkey by viral escape from cytotoxic T lymphocytes. Nature 415:335–339 [CrossRef]
    [Google Scholar]
  4. Bercovici N., Duffour M.-T., Agrawal S., Salcedo M., Abastado J.-P. 2000; New methods for assessing T-cell responses. Clin Diagn Lab Immunol 7:859–864
    [Google Scholar]
  5. Donahoe S. M., Moretto W. J., Samuel R. V., Metzner K. J., Marx P. A., Hanke T., Connor R. I., Nixon D. F. 2000; Direct measurement of CD8+ T cell responses in macaques infected with simian immunodeficiency virus. Virology 272:347–356 [CrossRef]
    [Google Scholar]
  6. Gauduin M.-C., Kaur A., Ahmad S., Yilma T., Lifson J. D., Johnson R. P. 2004; Optimization of intracellular cytokine staining for the quantitation of antigen-specific CD4+ T cell responses in rhesus macaques. J Immunol Methods 288:61–79 [CrossRef]
    [Google Scholar]
  7. Geretti A.-M., Hulskotte E. G. J., Dings M. E. M., van Baalen C. A., van Amerongen G., Osterhaus A. D. M. E. 1997; CD8 cytotoxic T lymphocytes of a cynomolgus macaque infected with simian immunodeficiency virus (SIV) mac32H-J5 recognize a nine amino acid epitope in SIV Gag p26. J Gen Virol 78:821–824
    [Google Scholar]
  8. Goulder P. J. R., Watkins D. I. 2004; HIV and SIV CTL escape: implications for vaccine design. Nat Rev Immunol 4:630–640 [CrossRef]
    [Google Scholar]
  9. Goulder P. J. R., Phillip R. E., Colbert R. A. & 9 other authors 1997; Late escape from an immunodominant cytotoxic T-lymphocyte response associated with progression to AIDS. Nat Med 3:212–217 [CrossRef]
    [Google Scholar]
  10. Harari A., Vallelian F., Meylan P. R., Pantaleo G. 2005; Functional heterogeneity of memory CD4 T cell responses in different conditions of antigen exposure and persistence. J Immunol 174:1037–1045 [CrossRef]
    [Google Scholar]
  11. Johnson R. P. 1996; Macaque models for AIDS vaccine development. Curr Opin Immunol 8:554–560 [CrossRef]
    [Google Scholar]
  12. Kaur A., Hale C. L., Noren B., Kassis N., Simon M. A., Johnson R. P. 2002; Decreased frequency of cytomegalovirus (CMV)-specific CD4+ T lymphocytes in simian immunodeficiency virus-infected rhesus macaques : inverse relationship with CMV viremia. J Virol 76:3646–3658 [CrossRef]
    [Google Scholar]
  13. Kuroda M. J., Schmitz J. E., Barouch D. H., Craiu A., Allen T. M., Sette A., Watkins D. I., Forman M. A., Letvin N. L. 1998; Analysis of Gag-specific cytotoxic T lymphocytes in simian immunodeficiency virus-infected rhesus monkeys by cell staining with a tetrameric major histocompatibility complex class I–peptide complex. J Exp Med 187:1373–1381 [CrossRef]
    [Google Scholar]
  14. Leuchte N., Berry N., Köhler B., Almond N., LeGrand R., Thorstensson R., Titti F., Sauermann U. 2004; MhcDRB -sequences from cynomolgus macaques ( Macaca fascicularis ) of different origin. Tissue Antigens 63:529–537 [CrossRef]
    [Google Scholar]
  15. Loffredo J. T., Sidney J., Wojewoda C. & 8 other authors 2004; Identification of seventeen new simian immunodeficiency virus-derived CD8+ T cell epitopes restricted by the high frequency molecule, Mamu-A*02, and potential escape from CTL recognition. J Immunol 173:5064–5076 [CrossRef]
    [Google Scholar]
  16. Menssen R., Orth P., Ziegler A., Saenger W. 1999; Decamer-like conformation of a nona-peptide bound to HLA-B*3501 due to non-standard positioning of the C terminus. J Mol Biol 285:645–653 [CrossRef]
    [Google Scholar]
  17. Miller M. D., Yamamoto H., Hughes A. L., Watkins D. I., Letvin N. L. 1991; Definition of an epitope and MHC class I molecule recognized by Gag-specific cytotoxic T lymphocytes in SIVmac-infected rhesus monkeys. J Immunol 147:320–329
    [Google Scholar]
  18. Mothé B. R., Sidney J., Dzuris J. L., Liebl M. E., Fuenger S., Watkins D. I., Sette A. 2002; Characterization of the peptide-binding specificity of Mamu-B*17 and identification of Mamu-B*17-restricted epitopes derived from simian immunodeficiency virus proteins. J Immunol 169:210–219 [CrossRef]
    [Google Scholar]
  19. Negri D. R. M., Buffa V., Leone P. & 7 other authors 2004; Use of retroviral vectors for the analysis of SIV/HIV-specific CD8 T cell responses. J Immunol Methods 291:153–163 [CrossRef]
    [Google Scholar]
  20. Ogg G. S., Jin X., Bonhoeffer S. & 12 other authors 1998; Quantitation of HIV-1-specific cytotoxic T lymphocytes and plasma load of viral RNA. Science 279:2103–2106 [CrossRef]
    [Google Scholar]
  21. Pantaleo G., Koup R. A. 2004; Correlates of immune protection in HIV-1 infection: what we know, what we don't know, what we should know. Nat Med 10:806–810 [CrossRef]
    [Google Scholar]
  22. Reid S. W., McAdam S., Smith K. J. & 8 other authors 1996; Antagonist HIV-1 Gag peptides induce structural changes in HLA B8. J Exp Med 184:2279–2286 [CrossRef]
    [Google Scholar]
  23. Robinson S., Charini W. A., Newberg M. H., Kuroda M. J., Lord C. I., Letvin N. L. 2001; A commonly recognized simian immunodeficiency virus nef epitope presented to cytotoxic T lymphocytes of Indian-origin rhesus monkeys by the pentavalent major histocompatibility complex class I allele Mamu-A*02. J Virol 75:10179–10186 [CrossRef]
    [Google Scholar]
  24. Schmitz J. E., Kuroda M. J., Santra S. & 13 other authors 1999; Control of viremia in simian immunodeficiency virus infection by CD8+ lymphocytes. Science 283:857–860 [CrossRef]
    [Google Scholar]
  25. Sun Y., Iglesias E., Samri A., Kamkamidze G., Decoville T., Carcelain G., Autran B. 2003; A systematic comparison of methods to measure HIV-1 specific CD8 T cells. J Immunol Methods 272:23–34 [CrossRef]
    [Google Scholar]
  26. Uda A., Tanabayashi K., Yamada Y. K., Akari H., Lee Y.-J., Mukai R., Terao K., Yamada A. 2004; Detection of 14 alleles derived from the MHC class I A locus in cynomolgus monkeys. Immunogenetics 56:155–163
    [Google Scholar]
  27. Uda A., Tanabayashi K., Fujita O., Hotta A., Terao K., Yamada A. 2005; Identification of the MHC class I B locus in cynomolgus monkeys. Immunogenetics 57:189–197 [CrossRef]
    [Google Scholar]
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