1887

Abstract

The whole-genome sequence of the nucleopolyhedrovirus (HycuNPV) was analysed. The entire nucleotide sequence of the HycuNPV genome was 132 959 bp long, with a G+C content of 45.1 mol%. A total of 148 open reading frames (ORFs) consisting of more than 50 aa were encoded by the genome. HycuNPV shares more than 122 ORFs with other lepidopteran group I NPVs, including , , (CfMNPV), defective NPV, and (OpMNPV). Six ORFs are identified as being unique to HycuNPV. Most of the HycuNPV ORFs showed higher similarity to CfMNPV and OpMNPV ORFs than to those of the other group I NPVs. HycuNPV encodes two conotoxin-like homologues (s), which are observed only in OpMNPV in group I NPVs. HycuNPV encodes three inhibitors of apoptosis (s), , and , a feature that it shares only with CfMNPV. In addition, six homologous regions (s) are identified in the HycuNPV genome. These s are located in regions similar to those of the OpMNPV s, but different from most of the CfMNPV s. Based on the close phylogenetic relationship and conservation of group I NPV-specific genes, such as , and , it is concluded that HycuNPV belongs to the group I NPVs and is most similar to CfMNPV or OpMNPV.

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2006-09-01
2024-04-24
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