1887

Abstract

The whole-genome sequence of the nucleopolyhedrovirus (HycuNPV) was analysed. The entire nucleotide sequence of the HycuNPV genome was 132 959 bp long, with a G+C content of 45.1 mol%. A total of 148 open reading frames (ORFs) consisting of more than 50 aa were encoded by the genome. HycuNPV shares more than 122 ORFs with other lepidopteran group I NPVs, including , , (CfMNPV), defective NPV, and (OpMNPV). Six ORFs are identified as being unique to HycuNPV. Most of the HycuNPV ORFs showed higher similarity to CfMNPV and OpMNPV ORFs than to those of the other group I NPVs. HycuNPV encodes two conotoxin-like homologues (s), which are observed only in OpMNPV in group I NPVs. HycuNPV encodes three inhibitors of apoptosis (s), , and , a feature that it shares only with CfMNPV. In addition, six homologous regions (s) are identified in the HycuNPV genome. These s are located in regions similar to those of the OpMNPV s, but different from most of the CfMNPV s. Based on the close phylogenetic relationship and conservation of group I NPV-specific genes, such as , and , it is concluded that HycuNPV belongs to the group I NPVs and is most similar to CfMNPV or OpMNPV.

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2006-09-01
2019-11-19
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References

  1. Ahrens, C. H., Russell, R. L. Q., Funk, C. J., Evans, J. T., Harwood, S. H. & Rohrmann, G. F. ( 1997; ). The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome. Virology 229, 381–399.[CrossRef]
    [Google Scholar]
  2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  3. Aruga, H., Yoshitake, N., Watanabe, H. & Hukuhara, T. ( 1960; ). Studies on nuclear polyhedroses and their inductions in some Lepidoptera. Jpn J Appl Entomol Zool 4, 51–56.[CrossRef]
    [Google Scholar]
  4. Cunningham, J. T. ( 1998; ). North America. In Insect Viruses and Pest Management, pp. 313–331. Edited by F. R. Hunter-Fujita, P. F. Entwistle, H. F. Evans & N. F. Crook. Chichester, England: Wiley.
  5. de Jong, J. G., Lauzon, H. A. M., Dominy, C., Poloumienko, A., Carstens, E. B., Arif, B. M. & Krell, P. J. ( 2005; ). Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome. J Gen Virol 86, 929–943.[CrossRef]
    [Google Scholar]
  6. Felipe Alves, C. A., Ikeda, M. & Kobayashi, M. ( 2002a; ). Identification and characterization of Hyphantria cunea nucleopolyhedrovirus homologous repeated regions. Virus Genes 25, 281–290.[CrossRef]
    [Google Scholar]
  7. Felipe Alves, C. A., Ikeda, M. & Kobayashi, M. ( 2002b; ). Characterization of Hyphantria cunea nucleopolyhedrovirus gp64 gene and analysis of elements regulating its early promoter activity. J Insect Biotechnol Sericol 71, 141–150.
    [Google Scholar]
  8. Herniou, E. A., Olszewski, J. A., Cory, J. S. & O'Reilly, D. R. ( 2003; ). The genome sequence and evolution of baculoviruses. Annu Rev Entomol 48, 211–234.[CrossRef]
    [Google Scholar]
  9. Herniou, E. A., Olszewski, J. A., O'Reilly, D. R. & Cory, J. S. ( 2004; ). Ancient coevolution of baculoviruses and their insect hosts. J Virol 78, 3244–3251.[CrossRef]
    [Google Scholar]
  10. Hukuhara, T. & Hashimoto, Y. ( 1966; ). Virus disease of Hyphantria cunea Drury. Jpn J Appl Entomol Zool 10, 149–155.[CrossRef]
    [Google Scholar]
  11. Hyink, O., Dellow, R. A., Olsen, M. J., Caradoc-Davies, K. M. B., Drake, K., Herniou, E. A., Cory, J. S., O'Reilly, D. R. & Ward, V. K. ( 2002; ). Whole genome analysis of the Epiphyas postvittana nucleopolyhedrovirus. J Gen Virol 83, 957–971.
    [Google Scholar]
  12. Ikeda, M., Yanagimoto, K. & Kobayashi, M. ( 2004; ). Identification and functional analysis of Hyphantria cunea nucleopolyhedrovirus iap genes. Virology 321, 359–371.[CrossRef]
    [Google Scholar]
  13. Ishikawa, H., Ikeda, M., Yanagimoto, K., Felipe Alves, C. A., Katou, Y., Laviña-Caoili, B. A. & Kobayashi, M. ( 2003; ). Induction of apoptosis in an insect cell line, IPLB-Ld652Y, infected with nucleopolyhedroviruses. J Gen Virol 84, 705–714.[CrossRef]
    [Google Scholar]
  14. Ishikawa, H., Ikeda, M., Felipe Alves, C. A., Thiem, S. M. & Kobayashi, M. ( 2004; ). Host range factor 1 from Lymantria dispar nucleopolyhedrovirus (NPV) is an essential viral factor required for productive infection of NPVs in IPLB-LD652Y cells derived from L. dispar. J Virol 78, 12703–12708.[CrossRef]
    [Google Scholar]
  15. Iwahori, S., Ikeda, M. & Kobayashi, M. ( 2002; ). Generation and characterization of Autographa californica nucleopolyhedrovirus mutants defective in pcna gene homologue. J Insect Biotechnol Sericol 71, 129–139.
    [Google Scholar]
  16. Kamiya, K., Okimoto, N., Ikeda, M., Kunimi, Y., Kobayashi, M. & Kawamura, S. ( 2003; ). Genotypic variation of a wild isolate of Hyphantria cunea nucleopolyhedrovirus. J Insect Biotechnol Sericol 72, 57–64.
    [Google Scholar]
  17. Kunimi, Y. ( 1998; ). Japan. In Insect Viruses and Pest Management, pp. 269–279. Edited by F. R. Hunter-Fujita, P. F. Entwistle, H. F. Evans & N. F. Crook. Chichester, England: Wiley.
  18. Kuzio, J., Pearson, M. N., Harwood, S. H., Funk, C. J., Evans, J. T., Slavicek, J. M. & Rohrmann, G. F. ( 1999; ). Sequence and analysis of the genome of a baculovirus pathogenic for Lymantria dispar. Virology 253, 17–34.[CrossRef]
    [Google Scholar]
  19. Lauzon, H. A. M., Jamieson, P. B., Krell, P. J. & Arif, B. M. ( 2005; ). Gene organization and sequencing of the Choristoneura fumiferana defective nucleopolyhedrovirus genome. J Gen Virol 86, 945–961.[CrossRef]
    [Google Scholar]
  20. Leisy, D. J., Rasmussen, C., Owusu, E. O. & Rohrmann, G. F. ( 1997; ). A mechanism for negative gene regulation in Autographa californica multinucleocapsid nuclear polyhedrosis virus. J Virol 71, 5088–5094.
    [Google Scholar]
  21. Miller, L. K. & Lu, A. ( 1997; ). The molecular basis of baculovirus host range. In The Baculoviruses, pp. 217–235. Edited by L. K. Miller. New York: Plenum.
  22. Mitsuhashi, J. & Inoue, H. ( 1988; ). Obtainment of a continuous cell line from the larval fat bodies of the mulberry tiger moth, Spilosoma imparilis (Lepidoptera: Arctiidae). Appl Entomol Zool 23, 488–490.
    [Google Scholar]
  23. Mitsuhashi, J. & Maramorosch, K. ( 1964; ). Leafhopper tissue culture: embryonic, nymphal, and imaginal tissues from aseptic insects. Contrib Boyce Thompson Inst 22, 435–460.
    [Google Scholar]
  24. Nagai, S., Felipe Alves, C. A., Shirata, N., Katou, Y., Ishikawa, H., Ikeda, M. & Kobayashi, M. ( 2006; ). hycu-hr6, a large homologous region of Hyphantria cunea nucleopolyhedrovirus, is dispensable for virus replication in culture cells. J Insect Biotechnol Sericol 74 (in press).
    [Google Scholar]
  25. O'Reilly, D. R., Miller, L. K. & Luckow, V. A. ( 1992; ). In Baculovirus Expression Vectors: a Laboratory Manual, p. 347. New York: W. H. Freeman & Company.
  26. Shirata, N., Ikeda, M., Kamiya, K., Kawamura, S. & Kobayashi, M. ( 2004; ). Restriction of Bombyx mori nucleopolyhedrovirus (NPV) replication by Hyphantria cunea NPV in a cell line from B. mori. J Insect Biotechnol Sericol 73, 23–33.
    [Google Scholar]
  27. Tautz, D. & Renz, M. ( 1984; ). Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Res 12, 4127–4138.[CrossRef]
    [Google Scholar]
  28. Theilmann, D. A., Blissard, G. W., Bonning, B., Jehle, J. O'Reilly D. R., Rohrmann, G. F., Thiem, S. & Vlak, J. M. ( 2005; ). Family Baculoviridae. In Virus Taxonomy: Eighth Report of the International Committee of Taxonomy of Viruses, pp. 177–185. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. San Diego, CA: Elsevier Academic Press.
  29. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  30. Washburn, J. O., Chan, E. Y., Volkman, L. E., Aumiller, J. J. & Jarvis, D. L. ( 2003; ). Early synthesis of budded virus envelope fusion protein GP64 enhances Autographa californica multicapsid nucleopolyhedrovirus virulence in orally infected Heliothis virescens. J Virol 77, 280–290.[CrossRef]
    [Google Scholar]
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vol. , part 9, pp. 2549 – 2562

Alignment and characterization of six s

Characteristics of group I NPV genomes

ORFs present in all group I NPV genomes

ORFs encoded by HycuNPV and some of the other group I NPVs

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