1887

Abstract

By using high-sensitivity Western blotting and immunohistochemistry, pituitary glands from patients with sporadic and variant Creutzfeldt–Jakob disease (sCJD and vCJD, respectively) were analysed for the presence of the protease-resistant form of the prion protein (PrP). PrP was detected in a greater proportion of vCJD pituitaries than sCJD pituitaries and was localized predominantly in the neurohypophysis. PrP was also detected in a recurrent pituitary adenoma from an sCJD patient. Immunohistochemical analysis showed sparse positive labelling, predominantly in folliculostellate cells, in vCJD and sCJD adenohypophyses. The PrP glycosylation pattern in the vCJD neurohypophyses showed a predominance of the unglycosylated band, which differed markedly from patterns found in all other vCJD tissues. The presence of PrP in the pituitary of CJD patients at autopsy suggests that human growth hormone-related iatrogenic CJD may have indeed resulted from infectivity in collected pituitaries rather than necessarily from contamination of pituitary pools by adjacent brain tissue.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81913-0
2007-03-01
2019-11-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/88/3/1068.html?itemId=/content/journal/jgv/10.1099/vir.0.81913-0&mimeType=html&fmt=ahah

References

  1. Brown, P., Brandel, J. P., Preese, M. & Sato, T. ( 2006; ). Iatrogenic Creutzfeldt-Jakob disease: the waning of an era. Neurology 67, 389–393.[CrossRef]
    [Google Scholar]
  2. Budka, H., Aguzzi, A., Brown, P., Brucher, J. M., Bugiani, O., Gullotta, F., Haltia, M., Hauw, J. J., Ironside, J. W. & other authors ( 1995; ). Neuropathological diagnostic criteria for Creutzfeldt-Jakob disease (CJD) and other human spongiform encephalopathies (prion diseases). Brain Pathol 5, 459–466.[CrossRef]
    [Google Scholar]
  3. Cervenakova, L., Yakovleva, O. & McKenzie, C. ( 2006; ). Protease-resistant prion protein in lymphoreticular tumors of variant Creutzfeldt-Jakob disease mice. Emerg Infect Dis 12, 511–513.[CrossRef]
    [Google Scholar]
  4. Collinge, J., Sidle, K. C., Meads, J., Ironside, J. & Hill, A. F. ( 1996; ). Molecular analysis of prion strain variation and the aetiology of ‘new variant’ CJD. Nature 383, 685–690.[CrossRef]
    [Google Scholar]
  5. Fauquier, T., Lacampagne, A., Travo, P., Bauer, K. & Mollard, P. ( 2002; ). Hidden face of the anterior pituitary. Trends Endocrinol Metab 13, 304–309.[CrossRef]
    [Google Scholar]
  6. Gambetti, P., Kong, Q., Zou, W., Parchi, P. & Chen, S. G. ( 2003; ). Sporadic and familial CJD: classification and characterisation. Br Med Bull 66, 213–239.[CrossRef]
    [Google Scholar]
  7. Gayrard, V., Picard-Hagen, N., Grino, M., Sauze, N., Grandjean, C., Galea, J., Andreoletti, O., Schelcher, F. & Toutain, P. L. ( 2000; ). Major hypercorticism is an endocrine feature of ewes with naturally occurring scrapie. Endocrinology 141, 988–994.[CrossRef]
    [Google Scholar]
  8. Gibbs, C. J., Jr, Asher, D. M., Brown, P. W., Fradkin, J. E. & Gajdusek, D. C. ( 1993; ). Creutzfeldt-Jakob disease infectivity of growth hormone derived from human pituitary glands. N Engl J Med 328, 358–359.
    [Google Scholar]
  9. Glatzel, M., Abela, E., Maissen, M. & Aguzzi, A. ( 2003; ). Extraneural pathologic prion protein in sporadic Creutzfeldt-Jakob disease. N Engl J Med 349, 1812–1820.[CrossRef]
    [Google Scholar]
  10. Head, M. W., Northcott, V., Rennison, K., Ritchie, D., McCardle, L., Bunn, T. J., McLennan, N. F., Ironside, J. W., Tullo, A. B. & Bonshek, R. E. ( 2003; ). Prion protein accumulation in eyes of patients with sporadic and variant Creutzfeldt-Jakob disease. Invest Ophthalmol Vis Sci 44, 342–346.[CrossRef]
    [Google Scholar]
  11. Head, M. W., Bunn, T. J., Bishop, M. T., McLoughlin, V., Lowrie, S., McKimmie, C. S., Williams, M. C., McCardle, L., MacKenzie, J. & other authors ( 2004a; ). Prion protein heterogeneity in sporadic but not variant Creutzfeldt-Jakob disease: UK cases 1991–2002. Ann Neurol 55, 851–859.[CrossRef]
    [Google Scholar]
  12. Head, M. W., Ritchie, D., Smith, N., McLoughlin, V., Nailon, W., Samad, S., Masson, S., Bishop, M., McCardle, L. & Ironside, J. W. ( 2004b; ). Peripheral tissue involvement in sporadic, iatrogenic, and variant Creutzfeldt-Jakob disease: an immunohistochemical, quantitative, and biochemical study. Am J Pathol 164, 143–153.[CrossRef]
    [Google Scholar]
  13. Head, M. W., Peden, A. H., Yull, H. M., Ritchie, D. L., Bonshek, R. E., Tullo, A. B. & Ironside, J. W. ( 2005; ). Abnormal prion protein in the retina of the most commonly occurring subtype of sporadic Creutzfeldt-Jakob disease. Br J Ophthalmol 89, 1131–1133.[CrossRef]
    [Google Scholar]
  14. Hilton, D. A., Ghani, A. C., Conyers, L., Edwards, P., McCardle, L., Ritchie, D., Penney, M., Hegazy, D. & Ironside, J. W. ( 2004; ). Prevalence of lymphoreticular prion protein accumulation in UK tissue samples. J Pathol 203, 733–739.[CrossRef]
    [Google Scholar]
  15. Ironside, J. W., Head, M. W., Bell, J. E., McCardle, L. & Will, R. G. ( 2000; ). Laboratory diagnosis of variant Creutzfeldt-Jakob disease. Histopathology 37, 1–9.[CrossRef]
    [Google Scholar]
  16. Ladogana, A., Puopolo, M., Croes, E. A., Budka, H., Jarius, C., Collins, S., Klug, G. M., Sutcliffe, T., Giulivi, A. & other authors ( 2005; ). Mortality from Creutzfeldt-Jakob disease and related disorders in Europe, Australia, and Canada. Neurology 64, 1586–1591.[CrossRef]
    [Google Scholar]
  17. Parchi, P., Castellani, R., Capellari, S., Ghetti, B., Young, K., Chen, S. G., Farlow, M., Dickson, D. W., Sima, A. A. & other authors ( 1996; ). Molecular basis of phenotypic variability in sporadic Creutzfeldt-Jakob disease. Ann Neurol 39, 767–778.[CrossRef]
    [Google Scholar]
  18. Peden, A. H., Head, M. W., Ritchie, D. L., Bell, J. E. & Ironside, J. W. ( 2004; ). Preclinical vCJD after blood transfusion in a PRNP codon 129 heterozygous patient. Lancet 364, 527–529.[CrossRef]
    [Google Scholar]
  19. Sabattini, E., Bisgaard, K., Ascani, S., Poggi, S., Piccioli, M., Ceccarelli, C., Pieri, F., Fraternali-Orcioni, G. & Pileri, S. A. ( 1998; ). The EnVision++ system: a new immunohistochemical method for diagnostics and research. Critical comparison with the APAAP, ChemMate, CSA, LABC, and SABC techniques. J Clin Pathol 51, 506–511.[CrossRef]
    [Google Scholar]
  20. Wadsworth, J. D., Joiner, S., Hill, A. F., Campbell, T. A., Desbruslais, M., Luthert, P. J. & Collinge, J. ( 2001; ). Tissue distribution of protease resistant prion protein in variant Creutzfeldt-Jakob disease using a highly sensitive immunoblotting assay. Lancet 358, 171–180.[CrossRef]
    [Google Scholar]
  21. Will, R. G. ( 2003; ). Acquired prion disease: iatrogenic CJD, variant CJD, kuru. Br Med Bull 66, 255–265.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.81913-0
Loading
/content/journal/jgv/10.1099/vir.0.81913-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error