1887

Abstract

Among the six subgroups of (ALV) that infect chickens, subgroup J (ALV-J) was isolated from meat-type chickens where it predominantly induces myeloid leukosis (ML) and erythroblastosis (EB). The sequence of HPRS-103, the ALV-J prototype virus, shows several distinct features, one of which is the presence of a distinct hairpin stem–loop structure called the E (also called XSR) element in the 3′ untranslated region. In order to determine the role of the E element in ALV-induced pathogenicity, a comparison was made of the oncogenicity of viruses derived from the provirus clones of parental and E element-deleted HPRS-103 viruses in two genetically distinct lines of birds. In line 15I birds, deletion of the E element had profound effects on virus replication , as only 55 % of birds showed evidence of infection, compared with 100 % infection by the parental virus. Furthermore, none of the line 15I birds infected with this virus developed tumours, indicating that the E element does contribute to the oncogenicity of the virus. On the other hand, deletion of the E element had only a marginal effect on the incidence of tumours in line 0 birds. These results indicate that, although the E element is not absolutely essential for tumour induction by this subgroup of viruses, it does contribute to oncogenicity in certain genetic lines of chicken.

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2006-09-01
2019-11-17
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References

  1. Bacon, L. D., Hunt, H. D. & Cheng, H. H. ( 2000; ). A review of the development of chicken lines to resolve genes determining resistance to diseases. Poult Sci 79, 1082–1093.[CrossRef]
    [Google Scholar]
  2. Bai, J., Payne, L. N. & Skinner, M. A. ( 1995; ). HPRS-103 (exogenous avian leukosis virus, subgroup J) has an env gene related to those of endogenous elements EAV-0 and E51 and an E element found previously only in sarcoma viruses. J Virol 69, 779–784.
    [Google Scholar]
  3. Benson, S. J., Ruis, B. L., Fadly, A. M. & Conklin, K. F. ( 1998; ). The unique envelope gene of the subgroup J avian leukosis virus derives from ev/J proviruses, a novel family of avian endogenous viruses. J Virol 72, 10157–10164.
    [Google Scholar]
  4. Bizub, D., Katz, R. A. & Skalka, A. M. ( 1984; ). Nucleotide sequence of noncoding regions in Rous-associated virus-2: comparisons delineate conserved regions important in replication and oncogenesis. J Virol 49, 557–565.
    [Google Scholar]
  5. Brown, D. W. & Robinson, H. L. ( 1988; ). Influence of env and long terminal repeat sequences on the tissue tropism of avian leukosis viruses. J Virol 62, 4828–4831.
    [Google Scholar]
  6. Brown, D. W., Blais, B. P. & Robinson, H. L. ( 1988; ). Long terminal repeat (LTR) sequences, env, and a region near the 5′ LTR influence the pathogenic potential of recombinants between Rous-associated virus types 0 and 1. J Virol 62, 3431–3437.
    [Google Scholar]
  7. Chai, N. & Bates, P. ( 2006; ). Na+/H+ exchanger type 1 is a receptor for pathogenic subgroup J avian leukosis virus. Proc Natl Acad Sci U S A 103, 5531–5536.[CrossRef]
    [Google Scholar]
  8. Chesters, P. M., Howes, K., McKay, J. C., Payne, L. N. & Venugopal, K. ( 2001; ). Acutely transforming avian leukosis virus subgroup J strain 966: defective genome encodes a 72-kilodalton Gag-Myc fusion protein. J Virol 75, 4219–4225.[CrossRef]
    [Google Scholar]
  9. Chesters, P. M., Howes, K., Petherbridge, L., Evans, S., Payne, L. N. & Venugopal, K. ( 2002; ). The viral envelope is a major determinant for the induction of lymphoid and myeloid tumours by avian leukosis virus subgroups A and J, respectively. J Gen Virol 83, 2553–2561.
    [Google Scholar]
  10. Cui, Z., Du, Y., Zhang, Z. & Silva, R. F. ( 2003; ). Comparison of Chinese field strains of avian leukosis subgroup J viruses with prototype strain HPRS-103 and United States strains. Avian Dis 47, 1321–1330.[CrossRef]
    [Google Scholar]
  11. Don, R. H., Cox, P. T., Wainwright, B. J., Baker, K. & Mattick, J. S. ( 1991; ). ‘Touchdown’ PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19, 4008.[CrossRef]
    [Google Scholar]
  12. Fadly, A. M. & Payne, L. N. ( 2003; ). Leukosis/sarcoma group. In Diseases of Poultry, 11th edn, pp. 465–516. Edited by Y. M. Saif, H. J. Barnes, J. R. Glisson, A. M. Fadly, L. R. McDougald & D. E. Swayne. Ames, IA: Iowa State Press.
  13. Fadly, A. M. & Witter, R. L. ( 1998; ). Oncornaviruses: leukosis/sarcoma and reticuloendotheliosis. In A Laboratory Manual for the Isolation and Identification of Avian Pathogens, 4th edn, pp. 185–196. Kennett Square, PA: American Association of Avian Pathologists.
  14. Fung, Y.-K. T., Lewis, W. G., Crittenden, L. B. & Kung, H.-J. ( 1983; ). Activation of the cellular oncogene c-erbB by LTR insertion: molecular basis for induction of erythroblastosis by avian leukosis virus. Cell 33, 357–368.[CrossRef]
    [Google Scholar]
  15. Gong, M., Semus, H. L., Bird, K. J., Stramer, B. J. & Ruddell, A. ( 1998; ). Differential selection of cells with proviral c-myc and c-erbB integrations after avian leukosis virus infection. J Virol 72, 5517–5525.
    [Google Scholar]
  16. Hayward, W. S., Neel, B. G. & Astrin, S. M. ( 1981; ). Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis. Nature 290, 475–480.[CrossRef]
    [Google Scholar]
  17. Higuchi, R. ( 1989; ). Using PCR to engineer DNA. In PCR Technology: Principles and Applications for DNA Amplification, pp. 61–70. Edited by H. A. Erlich. New York: Stockton Press.
  18. Laimins, L. A., Tsichlis, P. & Khoury, G. ( 1984; ). Multiple enhancer domains in the 3′ terminus of the Prague strain of Rous sarcoma virus. Nucleic Acids Res 12, 6427–6442.[CrossRef]
    [Google Scholar]
  19. Lupiani, B., Williams, S. M., Silva, R. F., Hunt, H. D. & Fadly, A. M. ( 2003; ). Pathogenicity of two recombinant avian leukosis viruses. Avian Dis 47, 425–432.[CrossRef]
    [Google Scholar]
  20. Payne, L. N., Gillespie, A. M. & Howes, K. ( 1991a; ). Induction of myeloid leukosis and other tumours with the HPRS-103 strain of ALV. Vet Rec 129, 447–448.[CrossRef]
    [Google Scholar]
  21. Payne, L. N., Brown, S. R., Bumstead, N., Howes, K., Frazier, J. A. & Thouless, M. E. ( 1991b; ). A novel subgroup of exogenous avian leukosis virus in chickens. J Gen Virol 72, 801–807.[CrossRef]
    [Google Scholar]
  22. Payne, L. N., Howes, K., Gillespie, A. M. & Smith, L. M. ( 1992; ). Host range of Rous sarcoma virus pseudotype RSV(HPRS-103) in 12 avian species: support for a new avian retrovirus envelope subgroup, designated J. J Gen Virol 73, 2995–2997.[CrossRef]
    [Google Scholar]
  23. Payne, L. N., Gillespie, A. M. & Howes, K. ( 1993; ). Recovery of acutely transforming viruses from myeloid leukosis induced by the HPRS-103 strain of avian leukosis virus. Avian Dis 37, 438–450.[CrossRef]
    [Google Scholar]
  24. Ruddell, A., Linial, M. L. & Groudine, M. ( 1989; ). Tissue-specific lability and expression of avian leukosis virus long terminal repeat enhancer-binding proteins. Mol Cell Biol 9, 5660–5668.
    [Google Scholar]
  25. Sacco, M. A., Flannery, D. M. J., Howes, K. & Venugopal, K. ( 2000; ). Avian endogenous retrovirus EAV-HP shares regions of identity with avian leukosis virus subgroup J and the avian retrotransposon ART-CH. J Virol 74, 1296–1306.[CrossRef]
    [Google Scholar]
  26. Sacco, M. A., Howes, K., Smith, L. P. & Nair, V. K. ( 2004; ). Assessing the roles of endogenous retrovirus EAV-HP in avian leukosis virus subgroup J emergence and tolerance. J Virol 78, 10525–10535.[CrossRef]
    [Google Scholar]
  27. Sambrook, J. & Russell, D. ( 2001; ). Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  28. Smith, E. J., Fadly, A. & Okazaki, W. ( 1979; ). An enzyme-linked immunosorbent assay for detecting avian leukosis-sarcoma viruses. Avian Dis 23, 698–707.[CrossRef]
    [Google Scholar]
  29. Smith, L. M., Toye, A. A., Howes, K., Bumstead, N., Payne, L. N. & Venugopal, K. ( 1999; ). Novel endogenous retroviral sequences in the chicken genome closely related to HPRS-103 (subgroup J) avian leukosis virus. J Gen Virol 80, 261–268.
    [Google Scholar]
  30. Sung, H. W., Reddy, S. M. & Fadly, A. M. ( 2002; ). High virus titer in feather pulp of chickens infected with subgroup J avian leukosis virus. Avian Dis 46, 281–286.[CrossRef]
    [Google Scholar]
  31. Venugopal, K. ( 1999; ). Avian leukosis virus subgroup J: a rapidly evolving group of oncogenic retroviruses. Res Vet Sci 67, 113–119.[CrossRef]
    [Google Scholar]
  32. Venugopal, K., Howes, K., Flannery, D. M. J. & Payne, L. N. ( 2000; ). Isolation of acutely transforming subgroup J avian leukosis viruses that induce erythroblastosis and myelocytomatosis. Avian Pathol 29, 327–332.[CrossRef]
    [Google Scholar]
  33. Zuker, M. ( 2003; ). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31, 3406–3415.[CrossRef]
    [Google Scholar]
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