1887

Abstract

Open reading frame 132 () of (HearNPV) is a homologue of infectivity factor 2 () of . Sequence analysis indicated that Ha132 encoded a protein of 383 aa with a predicted molecular mass of 44.5 kDa. Alignment of HA132 and its baculovirus homologues revealed that HA132 was highly conserved among baculoviruses, with 14 absolutely conserved cysteine residues. RT-PCR indicated that was first transcribed at 24 h post-infection. Western blot analysis showed that a 43 kDa band was detectable in HearNPV-infected HzAM1 cells from 36 h post-infection. Western blots also indicated that HA132 was a component of the occlusion-derived virus, but not of budded virus. Deletion of from HearNPV abolished infectivity, but had no effect on the infectivity of the budded virus phenotype.

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2006-09-01
2019-12-13
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