RT Journal Article SR Electronic(1) A1 Servais, Charlotte A1 Caillet-Fauquet, Perrine A1 Draps, Marie-Louise A1 Velu, Thierry A1 de Launoit, Yvan A1 Brandenburger, AnnickYR 2006 T1 Hypoxic-response elements in the oncolytic parvovirus Minute virus of mice do not allow for increased vector production at low oxygen concentration JF Journal of General Virology, VO 87 IS 5 SP 1197 OP 1201 DO https://doi.org/10.1099/vir.0.81754-0 PB Microbiology Society, SN 1465-2099, AB Vectors derived from the autonomous parvovirus Minute virus of mice, MVM(p), are promising tools for the gene therapy of cancer. The validation of their in vivo anti-tumour effect is, however, hampered by the difficulty to produce high-titre stocks. In an attempt to increase vector titres, host cells were subjected to low oxygen tension (hypoxia). It has been shown that a number of viruses are produced at higher titres under these conditions. This is the case, among others, for another member of the family Parvoviridae, the erythrovirus B19 virus. Hypoxia stabilizes a hypoxia-inducible transcription factor (HIF-1α) that interacts with a ‘hypoxia-responsive element’ (HRE), the consensus sequence of which (A/GCGTG) is present in the B19 and MVM promoters. Whilst the native P4 promoter was induced weakly in hypoxia, vector production was reduced dramatically, and adding HRE elements to the P4 promoter of the vector did not alleviate this reduction. Hypoxia has many effects on cell metabolism. Therefore, even if the P4 promoter is activated, the cellular factors that are required for the completion of the parvoviral life cycle may not be expressed., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81754-0