1887

Abstract

Circumstantial evidence suggests that the genome of (FBNYV), a nanovirus, consists of eight distinct, circular, single-stranded DNAs, each of about 1 kb and encoding only one protein. Here, the use of cloned full-length FBNYV DNAs for reproducing FBNYV-like symptoms in , the principal natural host of FBNYV, is reported. Characteristic symptoms of FBNYV infection were obtained in faba bean plants following biolistic DNA delivery or agroinoculation with all eight FBNYV DNAs. Although the eight different DNAs have been invariably detected in field samples infected with the various geographical FBNYV isolates, experimental infection with different combinations of fewer than eight DNAs also led to typical FBNYV symptoms. Even only five genome components, DNA-R, DNA-S, DNA-M, DNA-U1 and DNA-U2, were sufficient for inducing disease symptoms in upon agroinoculation. Symptomatic plants agroinoculated or bombarded with eight DNAs contained typical FBNYV virions; however, the virus was not transmitted by or , two efficient aphid vectors of FBNYV.

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2006-06-01
2020-01-28
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vol. , part 6, pp. 1735 – 1743

Construction of pairs of tandemly repeated copies of FBNYV DNAs within the same T-DNA of pBin 19.

Primers used to identify individual FBNYV DNAs by PCR.

Squash blot of newly developed leafs (3 weeks post-agroinoculation) hybridized with a DNA-S-specific probe.

The His -M-Rep protein interacts with human pRB .

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