@article{mbs:/content/journal/jgv/10.1099/vir.0.81742-0, author = "Martín, Susana and García, María Laura and Troisi, Antonella and Rubio, Luis and Legarreta, Gonzalo and Grau, Oscar and Alioto, Daniela and Moreno, Pedro and Guerri, José", title = "Genetic variation of populations of Citrus psorosis virus", journal= "Journal of General Virology", year = "2006", volume = "87", number = "10", pages = "3097-3102", doi = "https://doi.org/10.1099/vir.0.81742-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81742-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = " Citrus psorosis virus (CPsV), the type species of genus Ophiovirus, has a segmented, negative-stranded RNA genome. We examined the population structure and genetic variation of CPsV in three coding regions located in RNAs 1, 2 and 3, analysing 22 isolates from Argentina, California, Florida, Italy and Spain. Most isolates contained a predominant sequence and some minor variants. Estimations of the genetic diversity and phylogenetic clustering of isolates disclosed two populations, one comprising isolates from Spain, Italy, Florida and California and the other including the Argentinean isolates. Isolate CPV-4 (from Texas) included for comparison was distant from both groups, suggesting that it belongs to a third group. The low ratio between non-synonymous and synonymous nucleotide substitutions indicated strong selection for amino acid sequence conservation, particularly in the coat protein gene. Incongruent phylogenetic relationships in different genomic regions suggested that exchange of genomic segments may have contributed to CPsV evolution.", }