1887

Abstract

(CPsV), the type species of genus , has a segmented, negative-stranded RNA genome. We examined the population structure and genetic variation of CPsV in three coding regions located in RNAs 1, 2 and 3, analysing 22 isolates from Argentina, California, Florida, Italy and Spain. Most isolates contained a predominant sequence and some minor variants. Estimations of the genetic diversity and phylogenetic clustering of isolates disclosed two populations, one comprising isolates from Spain, Italy, Florida and California and the other including the Argentinean isolates. Isolate CPV-4 (from Texas) included for comparison was distant from both groups, suggesting that it belongs to a third group. The low ratio between non-synonymous and synonymous nucleotide substitutions indicated strong selection for amino acid sequence conservation, particularly in the coat protein gene. Incongruent phylogenetic relationships in different genomic regions suggested that exchange of genomic segments may have contributed to CPsV evolution.

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2006-10-01
2021-07-24
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References

  1. Alioto D., Malfitano M., Troisi A., Peluso A., Martín S., Milne R. G., Guerri J., Moreno P. 2003; Variability of the coat protein gene of Citrus psorosis virus in Campania, southern Italy. Arch Virol 148:2155–2166 [CrossRef]
    [Google Scholar]
  2. Barthe G. A., Ceccardi T. L., Manjunath K. L., Derrick K. S. 1998; Citrus psorosis virus: nucleotide sequencing of the coat protein gene and detection by hybridization and RT–PCR. J Gen Virol 79:1531–1537
    [Google Scholar]
  3. Bonnet J., Fraile A., Sacristán S., Malpica J. M., García-Arenal F. 2005; Role of recombination in the evolution of natural populations of Cucumber mosaic virus , a tripartite RNA plant virus. Virology 332:359–368 [CrossRef]
    [Google Scholar]
  4. Callaghan B., Dietzgen R. G. 2005; Nucleocapsid gene variability reveals two subgroups of Lettuce necrotic yellows virus . Arch Virol 150:1661–1667 [CrossRef]
    [Google Scholar]
  5. Chare E. R., Holmes E. C. 2004; Selection pressures in the capsid genes of plant RNA viruses reflect mode of transmission. J Gen Virol 85:3149–3157 [CrossRef]
    [Google Scholar]
  6. Derrick K. S., Brlansky R. H., da Graça J. V., Lee R. F., Timmer L. W., Nguyen T. K. 1988; Partial characterization of a virus associated with citrus ringspot. Phytopathology 78:1298–1301 [CrossRef]
    [Google Scholar]
  7. Domingo E., Holland J. J. 1994; Mutation rates and rapid evolution of RNA viruses. In The Evolutionary Biology of Viruses pp  161–168 Edited by Morse S. S. New York: Raven Press;
    [Google Scholar]
  8. Forster R. L. S., Beck D. L., Guilford P. J., Voot D. M., Vandolleweerd C. J., Andersen M. T. 1992; The coat protein of white clover mosaic potexvirus has a role in facilitating cell-to-cell transport in plants. Virology 191:480–484 [CrossRef]
    [Google Scholar]
  9. García M. L., Derrick K. S., Grau O. 1993; Citrus psorosis associated virus and citrus ringspot virus belong to a new virus group. In Proceedings of the 12th Conference of the International Organization of Citrus Virologists pp  430–431 Edited by Moreno P., da Graça J. V., Timmer L. W. Riverside, CA: IOCV;
    [Google Scholar]
  10. García M. L., Dal Bo E., Grau O., Milne R. 1994; The closely related citrus ringspot and citrus psorosis viruses have particles of novel filamentous morphology. J Gen Virol 75:3585–3590 [CrossRef]
    [Google Scholar]
  11. García-Arenal F., McDonald B. A. 2003; An analysis of the durability of resistance to plant viruses. Phytopathology 93:941–952 [CrossRef]
    [Google Scholar]
  12. García-Arenal F., Fraile A., Malpica J. M. 2001; Variability and genetic structure of plant virus populations. Annu Rev Phytopathol 39:157–186 [CrossRef]
    [Google Scholar]
  13. Garnsey S. M., Youtsey C. O., Bridges G. D., Burnett H. C. 1976; A necrotic ringspot-like virus found in a “Star Ruby” grapefruit tree imported without authorization from Texas. Proc Fla State Hort Soc 89:63–67
    [Google Scholar]
  14. Jakobsen I. B., Easteal S. 1996; A program for calculating and displaying compatibility matrices as an aid in determining reticulate evolution in molecular sequences. Comput Appl Biosci 12:291–295
    [Google Scholar]
  15. Kumar S., Tamura K., Jakobsen I.-B., Nei M. 2001 mega2: molecular evolutionary genetics analysis software Tempe, AZ: Arizona State University;
    [Google Scholar]
  16. Lu R., Folimonov A., Shintaku M., Li W. X., Falk B. W., Dawson W. O., Ding S. W. 2004; Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Natl Acad Sci U S A 101:15742–15747 [CrossRef]
    [Google Scholar]
  17. Lynch M., Crease T. J. 1990; The analysis of population survey data on DNA sequence variation. Mol Biol Evol 7:377–394
    [Google Scholar]
  18. Malpica J. M., Fraile A., Moreno I., Obies C. I., Drake J. W., García-Arenal F. 2002; The rate and character of spontaneous mutation in an RNA virus. Genetics 162:1505–1511
    [Google Scholar]
  19. Marco C. F., Aranda M. A. 2005; Genetic diversity of a natural population of Cucurbit yellow stunting disorder virus . J Gen Virol 86:815–822 [CrossRef]
    [Google Scholar]
  20. Martín S., Alioto D., Milne R. G., Garnsey S. M., García M. L., Grau O., Guerri J., Moreno P. 2004; Detection of Citrus psorosis virus by ELISA, molecular hybridization, RT-PCR and immunosorbent electron microscopy and its association with citrus psorosis disease. Eur J Plant Pathol 110:747–757 [CrossRef]
    [Google Scholar]
  21. Martín S., López C., García M. L., Naum-Onganía G., Grau O., Flores R., Moreno P., Guerri J. 2005; The complete nucleotide sequence of a Spanish isolate of Citrus psorosis virus : comparative analysis with other ophioviruses. Arch Virol 150:167–176 [CrossRef]
    [Google Scholar]
  22. Miranda G. J., Azzam O., Shirako Y. 2000; Comparison of nucleotide sequence between northern and southern Philippine isolates of Rice grassy stunt virus indicates occurrence of natural genetic reassortments. Virology 266:26–32 [CrossRef]
    [Google Scholar]
  23. Naum-Onganía G., Gago-Zachert S., Peña E., Grau O., García M. L. 2003; Citrus psorosis virus RNA 1 is of negative polarity and potentially encodes in its complementary strand a 24K protein of unknown function and 280K putative RNA dependent RNA polymerase. Virus Res 96:49–61 [CrossRef]
    [Google Scholar]
  24. Navarro J. A., Torok V. A., Vetten H. J., Pallás V. 2005; Genetic variability in the coat protein genes of lettuce big-vein associated virus and Mirafiori lettuce big-vein virus. Arch Virol 150:681–694 [CrossRef]
    [Google Scholar]
  25. Revill P., Trinh X., Dale J., Harding R. 2005; Taro vein chlorosis virus: characterization and variability of a new nucleorhabdovirus. J Gen Virol 86:491–499 [CrossRef]
    [Google Scholar]
  26. Rubio L., Ayllón M. A., Kong P., Fernández A., Polek M., Guerri J., Moreno P., Falk B. W. 2001; Genetic variation of Citrus tristeza virus isolates from California and Spain: evidence for mixed infections and recombination. J Virol 75:8054–8062 [CrossRef]
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Sánchez de la Torre E., Riva O., Zandomeni R., Grau O., García M. L. 1998; The top component of citrus psorosis virus contains two ssRNAs, the smaller encodes the coat protein. Mol Plant Pathol On-Line. http://www.bspp.org.uk/mppol/1998/1019sanchez/index.htm
  29. Sánchez de la Torre M. E., López C., Grau O., García M. L. 2002; RNA 2 of Citrus psorosis virus is of negative polarity and has a single open reading frame in its complementary strand. J Gen Virol 83:1777–1781
    [Google Scholar]
  30. Sawyer S. 1989; Statistical tests for detecting gene conversion. Mol Biol Evol 6:526–538
    [Google Scholar]
  31. Sneath P. H. A., Sackin M. J., Ambler R. P. 1975; Detecting evolutionary incompatibilities from protein sequences. Syst Zool 24:311–332 [CrossRef]
    [Google Scholar]
  32. Teycheney P.-Y., Laboureau N., Iskra-Caruana M.-L., Candresse T. 2005; High genetic variability and evidence for plant-to-plant transfer of Banana mild mosaic virus . J Gen Virol 86:3179–3187 [CrossRef]
    [Google Scholar]
  33. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  34. Turturo C., Saldarelli P., Yafeng D., Digiaro M., Minafra A., Savino V., Martelli G. P. 2005; Genetic variability and population structure of Grapevine leafroll-associated virus 3 isolates. J Gen Virol 86:217–224 [CrossRef]
    [Google Scholar]
  35. Vaira A. M., Accotto G. P., Gago-Zachert S. & 8 other authors 2004; Genus Ophiovirus . In Virus Taxonomy. Eighth Report of the International Committee on Taxonomy of Viruses pp  673–679 Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. London: Elsevier/Academic Press;
    [Google Scholar]
  36. Vigne E., Bergdoll M., Guyader S., Fuchs M. 2004; Population structure and genetic variability within isolates of Grapevine fanleaf virus from a naturally infected vineyard in France: evidence for mixed infection and recombination. J Gen Virol 85:2435–2445 [CrossRef]
    [Google Scholar]
  37. Vives M. C., Rubio L., Galipienso L., Navarro L., Moreno P., Guerri P. 2002; Low genetic variation between isolates of Citrus leaf blotch virus from different host species and of different geographical origins. J Gen Virol 83:2587–2591
    [Google Scholar]
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