1887

Abstract

The matrix (M) protein of paramyxoviruses forms an inner coat to the viral envelope and serves as a bridge between the surface glycoproteins (F and H) and the ribonucleoprotein core. Previously, a marker vaccine (RPV-PPRFH) was produced for the control of peste des petits ruminants (PPR) disease, where the F and H genes of (RPV) were replaced with the equivalent genes from (PPRV); however, this virus grew poorly in tissue culture. The poor growth of the RPV-PPRFH chimeric virus was thought to be due to non-homologous interaction of the surface glycoproteins with the internal components of the virus, in particular with the M protein. In contrast, replacement of the M gene of RPV with that from PPRV did not have an effect on the viability or replication efficiency of the recombinant virus. Therefore, in an effort to improve the growth of the RPV-PPRFH virus, a triple chimera (RPV-PPRMFH) was made, where the M, F and H genes of RPV were replaced with those from PPRV. As expected, the growth of the triple chimera was improved; it grew to a titre as high as that of the unmodified PPRV, although comparatively lower than that of the parental RPV virus. Goats infected with the triple chimera showed no adverse reaction and were protected from subsequent challenge with wild-type PPRV. The neutralizing-antibody titre on the day of challenge was ∼17 times higher than that in the RPV-PPRFH group, indicating RPV-PPRMFH as a promising marker-vaccine candidate.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81721-0
2006-07-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/7/2021.html?itemId=/content/journal/jgv/10.1099/vir.0.81721-0&mimeType=html&fmt=ahah

References

  1. Anderson J., McKay J. A. 1994; The detection of antibodies against peste des petits ruminants virus in cattle, sheep and goats and the possible implications to rinderpest control programmes. Epidemiol Infect 112:225–231 [CrossRef]
    [Google Scholar]
  2. Anderson J., Barrett T., Scott G. R. 1996 Manual on the Diagnosis of Rinderpest , 2nd edn. (FAO Animal Health Manual no 1 Rome: Food and Agriculture Organization of the United Nations;
    [Google Scholar]
  3. Bailey D., Banyard A., Dash P., Ozkul A., Barrett T. 2005; Full genome sequence of peste des petits ruminants virus, a member of the Morbillivirus genus. Virus Res 110:119–124 [CrossRef]
    [Google Scholar]
  4. Baron M. D., Barrett T. 2000; Rinderpest viruses lacking the C and V proteins show specific defects in growth and transcription of viral RNAs. J Virol 74:2603–2611 [CrossRef]
    [Google Scholar]
  5. Baron M. D., Foster-Cuevas M., Baron J., Barrett T. 1999; Expression in cattle of epitopes of a heterologous virus using a recombinant rinderpest virus. J Gen Virol 80:2031–2039
    [Google Scholar]
  6. Barrett T., Rossiter P. B. 1999; Rinderpest: the disease and its impact on humans and animals. Adv Virus Res 53:89–110
    [Google Scholar]
  7. Barrett T., Parida S., Mohapatra M., Walsh P., Das S., Baron M. D. 2003; Development of new generation rinderpest vaccines. Dev Biol (Basel) 114:89–97
    [Google Scholar]
  8. Bellini W. J., Englund G., Rozenblatt S., Arnheiter H., Richardson C. D. 1985; Measles virus P gene codes for two proteins. J Virol 53:908–919
    [Google Scholar]
  9. Cathomen T., Mrkic B., Spehner D., Drillien R., Naef R., Pavlovic J., Aguzzi A., Billeter M. A., Cattaneo R. 1998a; A matrix-less measles virus is infectious and elicits extensive cell fusion: consequences for propagation in the brain. EMBO J 17:3899–3908 [CrossRef]
    [Google Scholar]
  10. Cathomen T., Naim H. Y., Cattaneo R. 1998b; Measles viruses with altered envelope protein cytoplasmic tails gain cell fusion competence. J Virol 72:1224–1234
    [Google Scholar]
  11. Cattaneo R., Kaelin K., Baczko K., Billeter M. A. 1989; Measles virus editing provides an additional cysteine-rich protein. Cell 56:759–764 [CrossRef]
    [Google Scholar]
  12. Coronel E. C., Takimoto T., Murti K. G., Varich N., Portner A. 2001; Nucleocapsid incorporation into parainfluenza virus is regulated by specific interaction with matrix protein. J Virol 75:1117–1123 [CrossRef]
    [Google Scholar]
  13. Crowley J. C., Dowling P. C., Menonna J., Silverman J. I., Schuback D., Cook S. D., Blumberg B. M. 1988; Sequence variability and function of measles virus 3′ and 5′ ends and intercistronic regions. Virology 164:498–506 [CrossRef]
    [Google Scholar]
  14. Das S. C., Baron M. D., Barrett T. 2000a; Recovery and characterization of a chimeric rinderpest virus with the glycoproteins of peste-des-petits-ruminants virus: homologous F and H proteins are required for virus viability. J Virol 74:9039–9047 [CrossRef]
    [Google Scholar]
  15. Das S. C., Baron M. D., Skinner M. A., Barrett T. 2000b; Improved technique for transient expression and negative strand virus rescue using fowlpox T7 recombinant virus in mammalian cells. J Virol Methods 89:119–127 [CrossRef]
    [Google Scholar]
  16. Diallo A., Taylor W. P., Lefévre P. C., Provost A. 1989; Attenuation of a strain of rinderpest virus: potential homologous live vaccine. Rev Elev Med Vet Pays Trop 42:311–319 (in French
    [Google Scholar]
  17. Forsyth M. A., Barrett T. 1995; Evaluation of polymerase chain reaction for the detection and characterisation of rinderpest and peste des petits ruminants viruses for epidemiological studies. Virus Res 39:151–163 [CrossRef]
    [Google Scholar]
  18. Haffar A., Libeau G., Moussa A., Cécile M., Diallo A. 1999; The matrix protein gene sequence analysis reveals close relationship between peste des petits ruminants virus (PPRV) and dolphin morbillivirus. Virus Res 64:69–75 [CrossRef]
    [Google Scholar]
  19. Libeau G., Diallo A., Calvez D., Lefévre P. C. 1992; A competitive ELISA using anti-N monoclonal antibodies for specific detection of rinderpest antibodies in cattle and small ruminants. Vet Microbiol 31:147–160 [CrossRef]
    [Google Scholar]
  20. Libeau G., Prehaud C., Lancelot R., Colas F., Guerre L., Bishop D. H. L., Diallo A. 1995; Development of a competitive ELISA for detecting antibodies to the peste des petits ruminants virus using a recombinant nucleoprotein. Res Vet Sci 58:50–55 [CrossRef]
    [Google Scholar]
  21. Mahapatra M., Parida S., Egziabher B. G., Diallo A., Barrett T. 2003; Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus: editing of the gene transcript. Virus Res 96:85–98 [CrossRef]
    [Google Scholar]
  22. Mebatsion T., Weiland F., Conzelmann K.-K. 1999; Matrix protein of rabies virus is responsible for the assembly and budding of bullet-shaped particles and interacts with the transmembrane spike glycoprotein G. J Virol 73:242–250
    [Google Scholar]
  23. OIE 2000; Peste des petits ruminants. In Manual of Standards for Diagnostic Tests and Vaccines . , 4th edn. pp  114–122 Paris: Office International des Epizooties;
  24. Peeples M. E. 1991; Paramyxovirus M proteins: pulling it all together and taking it on the road. In The Paramyxoviruses pp  427–456 Edited by Kingsbury D. W. New York: Plenum;
    [Google Scholar]
  25. Plowright W., Ferris R. D. 1962; Studies with rinderpest virus in tissue culture. The use of attenuated culture virus as a vaccine for cattle. Res Vet Sci 3:172–182
    [Google Scholar]
  26. Sakaguchi T., Uchiyama T., Huang C., Fukuhara N., Kiyotani K., Nagai Y., Yoshida T. 2002; Alteration of Sendai virus morphogenesis and nucleocapsid incorporation due to mutation of cysteine residues of the matrix protein. J Virol 76:1682–1690 [CrossRef]
    [Google Scholar]
/content/journal/jgv/10.1099/vir.0.81721-0
Loading
/content/journal/jgv/10.1099/vir.0.81721-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error