1887

Abstract

Historically, coronaviruses have been recognized as a cause of minor respiratory infections in humans. However, the recent identification of three novel human coronaviruses, one causing severe acute respiratory syndrome (SARS), has prompted further examination of these viruses. Previous studies of geographically and chronologically distinct (HCoV-229E) isolates have found only limited variation within S gene nucleotide sequences. In contrast, analysis of the S genes of contemporary variants identified in Belgium revealed two distinct viruses circulating during 2003 and 2004. Here, the S and N gene sequences of 25 HCoV-229E variants identified in Victoria, Australia, between 1979 and 2004 in patients with symptomatic infections were determined. Phylogenetic analysis showed clustering of the isolates into four groups, with evidence of increasing divergence with time. Evidence of positive selection in the S gene was also established.

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2006-05-01
2020-04-06
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Supplements

vol. , part 5, pp. 1203 - 1208

Phylogenetic analysis using PHYLIP (DNAdist/neighbour-joining, 1000 bootstrap replicates) on HCoV-229E isolates and strains identified between 1972 and 2004

Primers used and their nucleotide position relative to the human coronavirus 229E complete genome

Summary of amino acid changes in the S protein of HCoV-229E variants studied

Summary of amino acid changes in N protein of HCoV-229E variants studied [Single PDF file](247 KB)



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