1887

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Volume 87, Issue 6

Characterization of a new densovirus infecting the German cockroach, Free

Abstract

A new DNA virus (: , s) was isolated and purified from descendants of field-collected German cockroaches, . Viral DNA and cockroach tissues infected with densovirus (DNV) were examined by electron microscopy. Virus particles, about 20 nm in diameter, were observed both in the nucleus and in the cytoplasm of infected cells. Virus DNA proved to be a linear molecule of about 1.2 μm in length. DNV isolated from infected cockroaches infected successfully and could be maintained in BGE-2, a cell line. The complete DNV genome was sequenced and analysed. Five open reading frames (ORFs) were detected in the 5335 nt sequence: two ORFS that were on one DNA strand encoded structural capsid proteins (69.7 and 24.8 kDa) and three ORFs that were on the other strand encoded non-structural proteins (60.2, 30.3 and 25.9 kDa). Three putative promoters and polyadenylation signals were identified. Structural analysis of the inverted terminal repeats revealed the presence of extended palindromes. The genome structure of DNV was compared with that of other members of the family ; the predicted amino acid sequences were aligned and subjected to phylogenetic analyses.

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2006-06-01
2024-04-19
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References

  1. Afanasiev B., Carlson J. 2000; Densovirinae as gene transfer vehicles. Contrib Microbiol 4:33–58
     [Google Scholar]
  2. Afanasiev B. N., Galyov E. E., Buchatsky L. P., Kozlov Y. V. 1991; Nucleotide sequence and genomic organization of Aedes densonucleosis virus. Virology 185:323–336 [CrossRef]
     [Google Scholar]
  3. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
     [Google Scholar]
  4. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
     [Google Scholar]
  5. Altukhov Y. P. 2003; Genetic Processes in Populations . , 3rd edn.p– 431 Moscow: Academkniga;
  6. Bando H., Choi H., Ito Y., Kawase S. 1990; Terminal structure of a densovirus implies a hairpin transfer replication which is similar to the model for AAV. Virology 179:57–63 [CrossRef]
     [Google Scholar]
  7. Bergoin M., Tijssen P. 2000; Molecular biology of Densovirinae . Contrib Microbiol 4:12–32
     [Google Scholar]
  8. Bloom M. E., Alexandersen S., Perryman S., Lechner D., Wolfinbarger J. B. 1988; Nucleotide sequence and genomic organization of Aleutian mink disease parvovirus (ADV): sequence comparisons between a nonpathogenic and a pathogenic strain of ADV. J Virol 62:2903–2915
     [Google Scholar]
  9. Bossin H., Fournier P., Royer C., Barry P., Cérutti P., Gimenez S., Couble P., Bergoin M. 2003; Junonia coenia densovirus-based vectors for stable transgene expression in Sf9 cells: influence of the densovirus sequences on genomic integration. J Virol 77:11060–11071 [CrossRef]
     [Google Scholar]
  10. Bucher P. 1990; Weight matrix descriptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences. J Mol Biol 212:563–578 [CrossRef]
     [Google Scholar]
  11. Bucher P., Trifonov E. N. 1986; Compilation and analysis of eukaryotic POL II promoter sequences. Nucleic Acids Res 14:10009–10026 [CrossRef]
     [Google Scholar]
  12. Carlson J., Afanasiev B., Suchman E. 2000; Densonucleosis virus as transducing vectors for insects. In Insect Transgenesis: Methods and Applications pp  139–159 Edited by Handler A. M., James A. A. New York: CRC Press;
     [Google Scholar]
  13. Chao Y. C., Young S. Y. III, Kim K. S. 1984; Cytopathology of the soybean looper, Pseudoplusia includen s, infected with the Pseudoplusia includens icosahedral virus. J Invertebr Pathol 45:16–23
     [Google Scholar]
  14. Chao Y. C., Young S. Y. III, Kim K. S., Scott H. A. 1985; A newly isolated densonucleosis virus from Pseudoplusia includens (Lepidoptera: Noctuidae). J Invertebr Pathol 46:70–82 [CrossRef]
     [Google Scholar]
  15. Corsini J., Afanasiev B., Maxwell I. H., Carlson J. O. 1996; Autonomous parvovirus and densovirus gene vectors. Adv Virus Res 47:303–351
     [Google Scholar]
  16. Deiss V., Tratschin J.-D., Weitz M., Siegl G. 1990; Cloning of the human parvovirus B19 genome and structural analysis of its palindromic termini. Virology 175:247–254 [CrossRef]
     [Google Scholar]
  17. Dumas B., Jourdan M., Pascaud A.-M., Bergoin M. 1992; Complete nucleotide sequence of the cloned infectious genome of Junonia coenia densovirus reveals an organization unique among parvoviruses. Virology 191:202–222 [CrossRef]
     [Google Scholar]
  18. Dykstra M. J., Reuss L. E. 2003 Biological Electron Microscopy: Theory, Techniques, and Troubleshooting , 2nd edn. New York: Springer;
     [Google Scholar]
  19. Fédière G. 2000; Epidemiology and pathology of Densovirinae . Contrib Microbiol 4:1–11
     [Google Scholar]
  20. Fédière G., El-Far M., Li Y., Bergoin M., Tijssen P. 2004; Expression strategy of densonucleosis virus from Mythimna loreyi . Virology 320:181–189 [CrossRef]
     [Google Scholar]
  21. Garzon S., Kurstak E. 1976; Ultrastructural studies on the morphogenesis of the densonucleosis virus (parvovirus). Virology 70:517–531 [CrossRef]
     [Google Scholar]
  22. Gross O., Tijssen P., Weinberg D., Tal J. 1990; Expression of densonucleosis virus Gm DNV in Galleria mellonella larvae: size analysis and in vitro translation of viral transcription products. J Invertebr Pathol 56:175–180 [CrossRef]
     [Google Scholar]
  23. Hu Y., Zheng J., Iizuka T., Bando H. 1994; A densovirus newly isolated from the smoky-brown cockroach Periplaneta fuliginosa . Arch Virol 138:365–372 [CrossRef]
     [Google Scholar]
  24. Kopanic R. J. Jr, Holbrook G. L., Sevala V., Schal C. 2001; An adaptive benefit of facultative coprophagy in the German cockroach Blattella germanica . Ecol Entomol 26:154–162 [CrossRef]
     [Google Scholar]
  25. Kurtti T. J., Brooks M. A. 1977; Isolation of cell lines from embryos of the cockroach, Blattella germanica . In Vitro 1311–17 [CrossRef]
     [Google Scholar]
  26. Kurtti T. J., Simser J. A., Baldridge G. D., Palmer A. T., Munderloh U. G. 2005; Factors influencing in vitro infectivity and growth of Rickettsia peacockii (Rickettsiales: Rickettsiaceae), an endosymbiont of the Rocky Mountain wood tick, Dermacentor andersoni (Acari, Ixodidae). J Invertebr Pathol 90:177–186 [CrossRef]
     [Google Scholar]
  27. Landureau J.-C., Jollès P. 1969; Etude des exigences d'une ligné de cellules d'insectes (souches) EPa. I. Acides amines. Exp Cell Res 54:391–398 (in French [CrossRef]
     [Google Scholar]
  28. Meynadier G., Matz G., Veyrunes J.-C., Bres N. 1977; Virose de type densonucleose chez les orthopteres. Ann Soc Entomol Fr (N S) 13:487–493 (in French
    [Google Scholar]
  29. Mukha D. V., Sidorenko A. P., Lazebnaya I. V., Zakharov I. A. 1995; Structural variation of the ribosomal gene cluster in the class Insecta. Genetika 31:1249–1253 (in Russian
    [Google Scholar]
  30. Munderloh U. G., Kurtti T. J. 1989; Formulation of medium for tick cell culture. Exp Appl Acarol 7:219–229 [CrossRef]
     [Google Scholar]
  31. Poland D. 1974; Recursion relation generation of probability profiles for specific-sequence macromolecules with long-range correlations. Biopolymers 13:1859–1871 [CrossRef]
     [Google Scholar]
  32. Reese M. G. 2001; Application of a time-delay neural network to promoter annotation in the Drosophila melanogaster genome. Comput Chem 26:51–56 [CrossRef]
     [Google Scholar]
  33. Rosenstreich D. L., Eggleston P., Kattan M. & 8 other authors 1997; The role of cockroach allergy and exposure to cockroach allergen in causing morbidity among inner-city children with asthma. N Engl J Med 336:1356–1363 [CrossRef]
     [Google Scholar]
  34. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  35. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74:5463–5467 [CrossRef]
     [Google Scholar]
  36. Shade R. O., Blundell M. C., Cotmore S. F., Tattersall P., Astell C. R. 1986; Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis. J Virol 58:921–936
     [Google Scholar]
  37. Shike H., Dhar A. K., Burns J. C., Shimizu C., Jousset F. X., Klimpel K. R., Bergoin M. 2000; Infectious hypodermal and hematopoietic necrosis virus of shrimp is related to mosquito brevidensoviruses. Virology 277:167–177 [CrossRef]
     [Google Scholar]
  38. Siegl G., Tratschin J. D. 1987; Parvoviruses: agents of distinct pathogenic and molecular potential. FEMS Microbiol Lett 46:433–450 [CrossRef]
     [Google Scholar]
  39. Smith R. F., Wiese B. A., Wojzynski M. K., Davison D. B., Worley K. C. 1996; BCM Search Launcher – an integrated interface to molecular biology data base search and analysis services available on the World Wide Web. Genome Res 6:454–462 [CrossRef]
     [Google Scholar]
  40. Srivastava A., Lusby E. W., Berns K. I. 1983; Nucleotide sequence and organization of the adeno-associated virus 2 genome. J Virol 45:555–564
     [Google Scholar]
  41. Steger G. 1994; Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction. Nucleic Acids Res 22:2760–2768 [CrossRef]
     [Google Scholar]
  42. Suto C., Kawamoto F., Kumada N. 1979; A new virus isolated from the cockroach, Periplaneta fuliginosa (Serville). Microbiol Immunol 23:207–211 [CrossRef]
     [Google Scholar]
  43. Tanada Y., Kaya H. K. 1993 Insect Pathology New York: Academic Press;
     [Google Scholar]
  44. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
     [Google Scholar]
  45. Tijssen P., Bergoin M. 1995; Densonucleosis viruses constitute an increasingly diversified subfamily among the parvoviruses. Semin Virol 6:347–355 [CrossRef]
     [Google Scholar]
  46. Tijssen P., Li Y., El-Far M., Szelei J., Letarte M., Zádori Z. 2003; Organization and expression strategy of the ambisense genome of densonucleosis virus of Galleria mellonella . J Virol 77:10357–10365 [CrossRef]
     [Google Scholar]
  47. Vago C., Duthoit J. L., Delahaye F. 1966; Les lésions nucléaires de la “Virose à noyaux denses” du lépidoptère Galleria mellonella . Arch Virol 18:344–349 (in French
    [Google Scholar]
  48. van Munster M., Dullemans A. M., Verbeek M., van den Heuvel J. F. J. M., Reinbold C., Brault V., Clérivet A., van der Wilk F. 2003; A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus . J Gen Virol 84:165–172 [CrossRef]
     [Google Scholar]
  49. Wang J., Zhang J., Jiang H., Liu C., Yi F., Hu Y. 2005; Nucleotide sequence and genomic organization of a newly isolated densovirus infecting Dendrolimus punctatus . J Gen Virol 86:2169–2173 [CrossRef]
     [Google Scholar]
  50. Yamagishi J., Hu Y., Zheng J., Bando H. 1999; Genome organization and mRNA structure of Periplaneta fuliginosa densovirus imply alternative splicing involvement in viral gene expression. Arch Virol 144:2111–2124 [CrossRef]
     [Google Scholar]
  51. Zuker M., Stiegler P. 1981; Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Res 9:133–148 [CrossRef]
     [Google Scholar]
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Characterization of a new densovirus infecting the German cockroach, Blattella germanica
J. Gen. Virol. 87, 1567 (2006); https://doi.org/10.1099/vir.0.81638-0
/content/journal/jgv/10.1099/vir.0.81638-0
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