1887

Abstract

In the family , two distinct envelope fusion proteins are identified in budded virions (BVs). GP64 is the major envelope fusion protein of group I nucleopolyhedrovirus (NPV) BVs. An unrelated type of envelope fusion protein, named F, is encoded by group II NPVs. The genome of (Hear) NPV, a group II NPV of the single nucleocapsid or S type, also encodes an F-like protein: open reading frame 133 (). It was demonstrated by N-terminal sequencing of the major 59 kDa protein present in HearNPV BV that this protein is one of the two F subunits: F (transmembrane subunit of 59 kDa) and F (surface subunit of 20 kDa), both the result of cleavage by a proprotein convertase and disulfide-linked. The HearNPV F protein proved to be a functional analogue of GP64, as the infectivity of an AcMNPV -deletion mutant was rescued by the introduction of the HearNPV F gene. It was also demonstrated by chemical cross-linking that HearNPV F is present in BVs as an oligomer whereby, unlike GP64, disulfide bonds are not involved. Deglycosylation assays indicated that both F and F possess -linked glycans. However, when F was made in Hz2E5 cells, these glycans did not have an -1-3 core fucose modification that usually occurs in insect cells. As -1-3 core fucose is a major inducer of an allergic response in humans, the present observation makes the HearNPV–Hz2E5 system an attractive alternative for the production of recombinant glycoproteins for therapeutic use in humans.

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2006-04-01
2019-11-18
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