1887

Abstract

reovirus (MpRV) is an 11-segmented, double-stranded RNA virus isolated from the marine protist . Sequence analysis (including conserved termini and presence of core motifs of reovirus polymerase), morphology and physicochemical properties confirmed the status of MpRV as a member of the family . Electron microscopy showed that intact virus particles are unusually larger (90–95 nm) than the known size of particles of viruses belonging to the family . Particles that were purified on caesium chloride gradients had a mean size of 75 nm (a size similar to the size of intact particles of members of the family ), indicating that they lost outer-coat components. The subcore particles had a mean size of 50 nm and a smooth surface, indicating that MpRV belongs to the non-turreted . The maximum amino acid identity with other reovirus proteins was 21 %, which is compatible with values existing between distinct genera. Based on morphological and sequence findings, this virus should be classified as the representative of a novel genus within the family , designated (from ). The topology of the phylogenetic tree built with putative polymerase sequences of the family suggested that the branch of MpRV could be ancestral. Further analysis showed that segment 1 of MpRV was much longer (5792 bp) than any other reovirus segment and encoded a protein of 200 kDa (VP1). This protein exhibited significant similarities to -glycosylated proteins, including viral envelope proteins, and is likely to represent the additional outer coat of MpRV.

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2006-05-01
2020-01-23
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References

  1. Attoui, H., Charrel, R. N., Billoir, F., Cantaloube, J.-F., de Micco, P. & de Lamballerie, X. ( 1998; ). Comparative sequence analysis of American, European and Asian isolates of viruses in the genus Coltivirus. J Gen Virol 79, 2481–2489.
    [Google Scholar]
  2. Attoui, H., Billoir, F., Cantaloube, J. F., Biagini, P., de Micco, P. & de Lamballerie, X. ( 2000a; ). Strategies for the sequence determination of viral dsRNA genomes. J Virol Methods 89, 147–158.[CrossRef]
    [Google Scholar]
  3. Attoui, H., Billoir, F., Biagini, P., Cantaloube, J. F., de Chesse, R., de Micco, P. & de Lamballerie, X. ( 2000b; ). Sequence determination and analysis of the full-length genome of Colorado tick fever virus, the type species of genus Coltivirus (family Reoviridae). Biochem Biophys Res Commun 273, 1121–1125.[CrossRef]
    [Google Scholar]
  4. Attoui, H., Fang, Q., Mohd Jaafar, F., Cantaloube, J.-F., Biagini, P., de Micco, P. & de Lamballerie, X. ( 2002a; ). Common evolutionary origin of aquareoviruses and orthoreoviruses revealed by genome characterization of Golden shiner reovirus, Grass carp reovirus, Striped bass reovirus and golden ide reovirus (genus Aquareovirus, family Reoviridae). J Gen Virol 83, 1941–1951.
    [Google Scholar]
  5. Attoui, H., Mohd Jaafar, F., Biagini, P., Cantaloube, J.-F., de Micco, P., Murphy, F. A. & de Lamballerie, X. ( 2002b; ). Genus Coltivirus (family Reoviridae): genomic and morphologic characterization of Old World and New World viruses. Arch Virol 147, 533–561.[CrossRef]
    [Google Scholar]
  6. Attoui, H., de Lamballerie, X. & Mertens, P. P. C. ( 2005; ). Coltivirus, Reoviridae. In Virus Taxonomy. Eighth Report of the International Committee on Taxonomy of Viruses, pp. 497–503. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier/Academic Press.
  7. Baker, T. S., Olson, N. H. & Fuller, S. D. ( 1999; ). Adding the third dimension to virus life cycles: three-dimensional reconstruction of icosahedral viruses from cryo-electron micrographs. Microbiol Mol Biol Rev 63, 862–922.
    [Google Scholar]
  8. Bhattacharya, D. & Medlin, L. ( 1998; ). Algal phylogeny and the origin of land plants. Plant Physiol 116, 9–15.[CrossRef]
    [Google Scholar]
  9. Brussaard, C. P. D., Noordeloos, A. A. M., Sandaa, R.-A., Heldal, M. & Bratbak, G. ( 2004; ). Discovery of a dsRNA virus infecting the marine photosynthetic protist Micromonas pusilla. Virology 319, 280–291.[CrossRef]
    [Google Scholar]
  10. Burroughs, J. N., O'Hara, R. S., Smale, C. J., Hamblin, C., Walton, A., Armstrong, R. & Mertens, P. P. C. ( 1994; ). Purification and properties of virus particles, infectious subviral particles, cores and VP7 crystals of African horsesickness virus serotype 9. J Gen Virol 75, 1849–1857.[CrossRef]
    [Google Scholar]
  11. Byrd, J. C. & Bresalier, R. S. ( 2004; ). Mucins and mucin binding proteins in colorectal cancer. Cancer Metastasis Rev 23, 77–99.[CrossRef]
    [Google Scholar]
  12. Cavalier-Smith, T. ( 2002; ). The phagotrophic origin of eukaryotes and phylogenetic classification of Protozoa. Int J Syst Evol Microbiol 52, 297–354.
    [Google Scholar]
  13. Cottrell, M. T. & Suttle, C. A. ( 1991; ). Wide-spread occurrence and clonal variation in viruses which cause lysis of a cosmopolitan, eukaryotic marine phytoplankter, Micromonas pusilla. Mar Ecol Prog Ser 78, 1–9.[CrossRef]
    [Google Scholar]
  14. Diprose, J. M., Burroughs, J. N., Sutton, G. C. & 8 other authors ( 2001; ). Translocation portals for the substrates and products of a viral transcriptase complex: the bluetongue virus core. EMBO J 20, 7229–7239.[CrossRef]
    [Google Scholar]
  15. Doolittle, W. F. ( 1999; ). Phylogenetic classification and the universal tree. Science 284, 2124–2128.[CrossRef]
    [Google Scholar]
  16. Estes, M. K. & Cohen, J. ( 1989; ). Rotavirus gene structure and function. Microbiol Rev 53, 410–449.
    [Google Scholar]
  17. Gibbs, A. & Keese, P. K. ( 1995; ). In search of the origins of viral genes. In Molecular Basis of Virus Evolution, pp. 76–90. Edited by A. Gibbs, C. H. Calisher & F. García-Arenal. Cambridge: Cambridge University Press.
  18. Gouet, P., Diprose, J. M., Grimes, J. M., Malby, R., Burroughs, J. N., Ziéntara, S., Stuart, D. I. & Mertens, P. P. C. ( 1999; ). The highly ordered double-stranded RNA genome of bluetongue virus revealed by crystallography. Cell 97, 481–490.[CrossRef]
    [Google Scholar]
  19. Grimes, J. M., Burroughs, J. N., Gouet, P., Diprose, J. M., Malby, R., Ziéntara, S., Mertens, P. P. C. & Stuart, D. I. ( 1998; ). The atomic structure of the bluetongue virus core. Nature 359, 470–478.
    [Google Scholar]
  20. Harrison, P. J., Waters, R. E. & Taylor, F. J. R. ( 1980; ). A broad spectrum artificial sea water medium for coastal and open ocean phytoplankton. J Phycol 16, 28–35.
    [Google Scholar]
  21. Hill, C. L., Booth, T. F., Prasad, B. V. V., Grimes, J. M., Mertens, P. P. C., Sutton, G. C. & Stuart, D. I. ( 1999; ). The structure of a cypovirus and the functional organization of dsRNA viruses. Nat Struct Biol 6, 565–568.[CrossRef]
    [Google Scholar]
  22. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  23. Martin, L. A., Meyer, A. J., O'Hara, R. S., Fu, H., Knowles, N. J. & Mertens, P. P. C. ( 1998; ). Phylogenetic analysis of African horse sickness virus genome segment 10: sequence variation, virulence characteristics and cell exit. Arch Virol Suppl 14, 281–293.
    [Google Scholar]
  24. Mertens, P. ( 2004; ). The dsRNA viruses. Virus Res 101, 3–13.[CrossRef]
    [Google Scholar]
  25. Mertens, P. P. C. & Diprose, J. ( 2004; ). The bluetongue virus core: a nano-scale transcription machine. Virus Res 101, 29–43.[CrossRef]
    [Google Scholar]
  26. Mertens, P. P. C., Arella, M., Attoui, H. & 41 other authors ( 2000; ). Reoviridae. In Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses, pp. 395–480. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. New York, NY: Academic Press.
  27. Mertens, P. P. C., Attoui, H., Duncan, R. & Dermody, T. S. ( 2005; ). Reoviridae. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, pp. 447–454. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier/Academic Press.
  28. Mohd Jaafar, F., Attoui, H., de Micco, P. & de Lamballerie, X. ( 2004; ). Termination and read-through proteins encoded by genome segment 9 of Colorado tick fever virus. J Gen Virol 85, 2237–2244.[CrossRef]
    [Google Scholar]
  29. Mohd Jaafar, F., Attoui, H., Mertens, P., de Micco, P. & de Lamballerie, X. ( 2005; ). Structural organisation of a human encephalitic isolate of Banna virus (genus Seadornavirus, family Reoviridae). J Gen Virol 86, 1147–1157.[CrossRef]
    [Google Scholar]
  30. Murphy, F. A., Coleman, P. H., Harrison, A. K. & Gary, G. W., Jr ( 1968; ). Colorado tick fever virus: an electron microscopic study. Virology 35, 28–40.[CrossRef]
    [Google Scholar]
  31. Nason, E. L., Rothagel, R., Mukherjee, S. K., Kar, A. K., Forzan, M., Prasad, B. V. & Roy, P. ( 2004; ). Interactions between the inner and outer capsids of bluetongue virus. J Virol 78, 8059–8067.[CrossRef]
    [Google Scholar]
  32. Owens, R. J., Limn, C. & Roy, P. ( 2004; ). Role of an arbovirus nonstructural protein in cellular pathogenesis and virus release. J Virol 78, 6649–6656.[CrossRef]
    [Google Scholar]
  33. Prasad, B. V. V., Wang, G. J., Clerx, J. P. M. & Chiu, W. ( 1988; ). Three-dimensional structure of rotavirus. J Mol Biol 199, 269–275.[CrossRef]
    [Google Scholar]
  34. Rasmussen, S. W. ( 1995; ). DNATools, a software package for DNA sequence analysis. Carlsberg Laboratory, Copenhagen.
  35. Reinisch, K. M., Nibert, M. L. & Harrison, S. C. ( 2000; ). Structure of the reovirus core at 3.6 Å resolution. Nature 404, 960–967.[CrossRef]
    [Google Scholar]
  36. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  37. Wommack, K. E., Ravel, J., Hill, R. T., Chun, J. & Colwell, R. R. ( 1999; ). Population dynamics of Chesapeake Bay virioplankton: total-community analysis by pulsed-field gel electrophoresis. Appl Environ Microbiol 65, 231–240.
    [Google Scholar]
  38. Yeager, M., Dryden, K. A., Olson, N. H., Greenber, H. B. & Baker, T. S. ( 1990; ). Three-dimensional structure of rhesus rotavirus by cryoelectron microscopy reconstruction. J Cell Biol 110, 2133–2144.[CrossRef]
    [Google Scholar]
  39. Yeager, M., Berriman, J. A., Baker, T. A. & Bellamy, A. R. ( 1994; ). Three-dimensional structure of the rotavirus haemagglutinin VP4 by cryo-electron microscopy and difference map analysis. EMBO J 13, 1011–1018.
    [Google Scholar]
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