@article{mbs:/content/journal/jgv/10.1099/vir.0.81570-0, author = "Xie, Xixian and Yang, Feng", title = "White spot syndrome virus VP24 interacts with VP28 and is involved in virus infection", journal= "Journal of General Virology", year = "2006", volume = "87", number = "7", pages = "1903-1908", doi = "https://doi.org/10.1099/vir.0.81570-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81570-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = " White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. Herein, the characterization of VP24, a major structural protein of WSSV, is described. When purified virions were subjected to Nonidet P-40 treatment to separate the envelopes from the nucleocapsids, VP24 was found to be present exclusively in the envelope fraction. Triton X-114 extraction also indicated that VP24 behaves as an envelope protein. Immunoelectron microscopy further confirmed that VP24 is located in the virion envelope. Far-Western experiments showed that VP24 interacts with VP28, another major envelope protein of the WSSV virion. To investigate the function of VP24, WSSV was neutralized with various amounts of anti-VP24 IgG and injected into crayfish. The results showed that anti-VP24 IgG could partially attenuate infection with WSSV. It is concluded that VP24 is an envelope protein and functions at an early stage in virus infection.", }