1887

Abstract

Endogenous retroviral sequences in the pig genome represent a potential infectious risk in xenotransplantation. Porcine endogenous retrovirus (PERV) sequences described to date have been classified into several families. The known infectious, human-tropic PERVs have been assigned to the PERV 1 subfamilies A, B and C. High copy numbers and full-length clones have also been observed for an additional family, designated PERV 2. The aim of this study was to examine the PERV 2 family by analysis of retroviral / gene sequences. The proviral load was observed to be similar among various pig breeds. Although clones harbouring an open reading frame in the examined region were found, analysis of published large PERV 2 clones revealed multiple deleterious mutations in each of the retroviral genes. Various recombination events between 2 genomes were revealed. In contrast to PERV 1, phylogenetic analyses did not distinguish defined subfamilies, but indicated the independent evolution of the proviruses after a single event of retroviral amplification. Expression analysis showed large PERV 2 transcripts and variable transcription in several tissues. Analysis of the two published 2 gene sequences observed the partial lack of the receptor-binding domain. Overall, this study indicated the low infectious potential for PERV 2.

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2006-04-01
2019-11-18
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