RT Journal Article SR Electronic(1) A1 Zhang, Qiwei A1 Su, Xiaobo A1 Gong, Sitang A1 Zeng, Qiyi A1 Zhu, Bing A1 Wu, Zaohe A1 Peng, Tao A1 Zhang, Chuyu A1 Zhou, RongYR 2006 T1 Comparative genomic analysis of two strains of human adenovirus type 3 isolated from children with acute respiratory infection in southern China JF Journal of General Virology, VO 87 IS 6 SP 1531 OP 1541 DO https://doi.org/10.1099/vir.0.81515-0 PB Microbiology Society, SN 1465-2099, AB Human adenovirus type 3 (HAdV-3) is a causative agent of acute respiratory disease, which is prevalent throughout the world, especially in Asia. Here, the complete genome sequences of two field strains of HAdV-3 (strains GZ1 and GZ2) isolated from children with acute respiratory infection in southern China are reported (GenBank accession nos DQ099432 and DQ105654, respectively). The genomes were 35 273 bp (GZ1) and 35 269 bp (GZ2) and both had a G+C content of 51 mol%. They shared 99 % nucleotide identity and the four early and five late regions that are characteristic of human adenoviruses. Thirty-nine protein- and two RNA-coding sequences were identified in the genome sequences of both strains. Protein pX had a predicted molecular mass of 8.3 kDa in strain GZ1; this was lower (7.6 kDa) in strain GZ2. Both strains contained 10 short inverted repeats, in addition to their inverted terminal repeats (111 bp). Comparative whole-genome analysis revealed 93 mismatches and four insertions/deletions between the two strains. Strain GZ1 infection produced a typical cytopathic effect, whereas strain GZ2 did not; non-synonymous substitutions in proteins of GZ2 may be responsible for this difference., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81515-0