1887

Abstract

Recombinant viruses based on modified vaccinia virus Ankara (MVA) are vaccine candidates against infectious diseases and cancers. Presently, multiplication of MVA has been demonstrated in chicken embryo fibroblast and baby hamster kidney (BHK-21) cells only. The multiplication and morphogenesis of a recombinant (MVA-HANP) and non-recombinant MVA strain in BHK-21 and 12 other mammalian cell lines have now been compared. Rat IEC-6 cells were fully permissive to MVA infection. The virus yield in IEC-6 cells was similar to that obtained in BHK-21 cells at low as well as high multiplicities of infection. Vero cells were semi-permissive to MVA infection. Mature virions were produced in supposedly non-permissive cell lines. The multiplication and morphogenesis of non-recombinant MVA and MVA-HANP were similar. These results are relevant to the production and biosafety of MVA-vectored vaccines.

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2006-01-01
2019-11-15
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vol. , part 1, pp. 21 - 27

Viral forms produced in IEC-6 cells infected with MVA-HANP as visualized by electron microscopy

Thin section of IEC-6 cells infected with non-recombinant MVA (ATCC VR-1508) at an m.o.i. of 5 IU per cell

Mature virions present in Vero and A549 cells infected with non-recombinant MVA (ATCC VR 1508) [Single PDF file](1295 KB)



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