Zygocactus virus X-based expression vectors and formation of rod-shaped virus-like particles in plants by the expressed coat proteins of Beet necrotic yellow vein virus and Soil-borne cereal mosaic virus
Expression vectors were constructed from 35S promoter-containing full-length cDNA clones of Zygocactus virus X (ZVX). The expression of foreign genes was driven by the ZVX coat protein (cp) subgenomic promoter. It was successful only when the variable region downstream of the conserved putative promoter region GSTTAAGTT(X12–13)GAA was retained. Most of the ZVX cp gene, except for a short 3′ part, was replaced by the corresponding sequence of the related Schlumbergera virus X (SVX) and its cp subgenomic promoter to enable encapsidation of the transcribed RNA by an SVX/ZVX hybrid cp. Vector-expressed cp of Beet necrotic yellow vein virus (BNYVV) assembled in Chenopodium quinoa, Tetragonia expansa and Beta vulgaris leaves into particles resembling true BNYVV particles. The virus produced from these constructs retained its ability to express BNYVV cp in local infections during successive passages on C. quinoa. This ability was lost, however, in the rarely occurring systemic infections.
AwramP.,
GardnerR. C.,
ForsterR. L.,
BellamyA. R.2002; The potential of plant viral vectors and transgenic plants for subunit vaccine production. Adv Virus Res 58:81–124
ChenJ.,
ShiY.-H.,
AdamsM. J.,
ChenJ.-P.2005; The complete sequence of the genomic RNA of an isolate of Lily virus X (genus Potexvirus ). Arch Virol 150:825–832[CrossRef]
DonsonJ.,
KearneyC. M.,
HilfM. E.,
DawsonW. O.1991; Systemic expression of a bacterial gene by a tobacco mosaic virus-based vector. Proc Natl Acad Sci U S A 88:7204–7208[CrossRef]
FlatkenS.,
MaissE.2005; Construction and agroinfection of a Potato virus M full-length clone. In Fourth Joint Meeting of Dutch and German Plant Virologists Wageningen: 10–11 March 2005p– 22
HaeberléA.-M.,
Stussi-GaraudC.,
SchmittC.,
GaraudJ.-C.,
RichardsK. E.,
GuilleyH.,
JonardG.1994; Detection by immunogold labelling of P75 readthrough protein near an extremity of beet necrotic yellow vein virus particles. Arch Virol 134:195–203[CrossRef]
KoenigR.,
SteinB.1990; Distribution of beet necrotic yellow vein virus in mechanically inoculated sugarbeet plantlets of cultivars with different degrees of rhizomania resistance. In Proceedings of the First Symposium of the International Working Group on Plant Viruses with Fungal Vectors Braunschweig, Germany: 21–24 August 1990 pp 87–90
KoenigR.,
PleijC. W. A.,
LossS.,
BurgermeisterW.,
AustH.,
SchiemannJ.2004; Molecular characterisation of potexviruses isolated from three different genera in the family Cactaceae
. Arch Virol 149:903–914[CrossRef]
MemelinkJ.,
van der VlugtC. I. M.,
LinthorstH. J. M.,
DerksA. F. L. M.,
AsjesC. J.,
BolJ. F.1990; Homologies between the genomes of a carlavirus (lily symptomless virus) and a potexvirus (lily virus X) from lily plants. J Gen Virol 71:917–924[CrossRef]
MilicicD.,
PleseN.,
BercksR.,
BrandesJ.,
CasperR.,
ChessinM.1966; Vergleichende serologische und elektronenmikroskopische Untersuchungen an Isolaten des Kakteen-X-Virus. Phytopathol Z 55:211–217 (in German [CrossRef]
NaylorM.,
ReevesJ.,
CooperJ. I.,
EdwardsM.-L.,
WangH.2005; Construction and properties of a gene-silencing vector based on Poplar mosaic virus (genus Carlavirus ). J Virol Methods 124:27–36[CrossRef]
PogueG. P.,
LindboJ. A.,
GargerS. J.,
FitzmauriceW. P.2002; Making an ally from an enemy: plant virology and the new agriculture. Annu Rev Phytopathol 40:45–74[CrossRef]
SchmittC.,
BalmoriE.,
JonardG.,
RichardsK. E.,
GuilleyH.1992; In vitro mutagenesis of biologically active transcripts of beet necrotic yellow vein virus RNA 2: evidence that a domain of the 75-kDa readthrough protein is important for efficient virus assembly. Proc Natl Acad Sci U S A 89:5715–5719[CrossRef]
TothR. L.,
PogueG. P.,
ChapmanS.2002; Improvement of the movement and host range properties of a plant virus vector through DNA shuffling. Plant J 30:593–600[CrossRef]
YamamiyaA.,
ShirakoY.2000; Construction of full-length cDNA clones to Soil-borne wheat mosaic virus RNA1 and RNA2 from which infectious cDNAs are transcribed in vitro : virion formation and systemic infection without expression of the N-terminal and C-terminal extensions to the capsid protein. Virology 277:66–75[CrossRef]
Zygocactus virus X-based expression vectors and formation of rod-shaped virus-like particles in plants by the expressed coat proteins of Beet necrotic yellow vein virus and Soil-borne cereal mosaic virus