1887

Abstract

(AlHV-1), carried asymptomatically by wildebeest, causes malignant catarrhal fever (MCF) following cross-species transmission to a variety of susceptible species of the order Artiodactyla. The study of MCF pathogenesis has been impeded by an inability to produce recombinant virus, mainly due to the fact that AlHV-1 becomes attenuated during passage in culture. In this study, these difficulties were overcome by cloning the entire AlHV-1 genome as a stable, infectious and pathogenic bacterial artificial chromosome (BAC). A modified -flanked BAC cassette was inserted in one of the two large non-coding regions of the AlHV-1 genome. This insertion allowed the production of an AlHV-1 BAC clone stably maintained in bacteria and able to regenerate virions when transfected into permissive cells. The -flanked BAC cassette was excised from the genome of reconstituted virions by growing them in permissive cells stably expressing Cre recombinase. Importantly, BAC-derived AlHV-1 virions replicated comparably to the virulent (low-passage) AlHV-1 parental strain and induced MCF in rabbits that was indistinguishable from that of the virulent parental strain. The availability of the AlHV-1 BAC is an important advance for the study of MCF that will allow the identification of viral genes involved in MCF pathogenesis, as well as the production of attenuated recombinant candidate vaccines.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81465-0
2006-03-01
2019-11-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/3/509.html?itemId=/content/journal/jgv/10.1099/vir.0.81465-0&mimeType=html&fmt=ahah

References

  1. Adler, H., Messerle, M., Wagner, M. & Koszinowski, U. H. ( 2000; ). Cloning and mutagenesis of the murine gammaherpesvirus 68 genome as an infectious bacterial artificial chromosome. J Virol 74, 6964–6974.[CrossRef]
    [Google Scholar]
  2. Barnard, B. J. ( 1990; ). Epizootology of wildebeest-derived malignant catarrhal fever: possible transmission among cows and their calves in the north-western Transvaal. Onderstepoort J Vet Res 57, 201–204.
    [Google Scholar]
  3. Bedelian, C. ( 2004; ). The impact of malignant catarrhal fever on Maasai pastoral communities in Kitengela Wildlife Dispersal Area, Kenya. MSc thesis, University of Edinburgh, UK.
  4. Borst, E.-M., Hahn, G., Koszinowski, U. H. & Messerle, M. ( 1999; ). Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: a new approach for construction of HCMV mutants. J Virol 73, 8320–8329.
    [Google Scholar]
  5. Burrells, C. & Reid, H. W. ( 1991; ). Phenotypic analysis of lymphoblastoid cell lines derived from cattle and deer affected with “sheep-associated” malignant catarrhal fever. Vet Immunol Immunopathol 29, 151–161.[CrossRef]
    [Google Scholar]
  6. Buxton, D. & Reid, H. W. ( 1980; ). Transmission of malignant catarrhal fever to rabbits. Vet Rec 106, 243–245.[CrossRef]
    [Google Scholar]
  7. Castro, A. E. & Heuschele, W. P. ( 1985; ). Conference on malignant catarrhal fever. Bovine Pract 20, 162–168.
    [Google Scholar]
  8. Castro, A. E., Ramsay, E. C., Dotson, J. F., Schramke, M. L., Kocan, A. A. & Whitenack, D. L. ( 1984; ). Characteristics of the herpesvirus of malignant catarrhal fever isolated from captive wildebeest calves. Am J Vet Res 45, 409–415.
    [Google Scholar]
  9. Cleaveland, S., Kusiluka, L., ole Kuwai, J., Bell, C. & Kazwala, R. ( 2001; ). Assessing the impact of malignant catarrhal fever in Ngorongoro district, Tanzania. Nairobi: Community-based Animal Health and Participatory Epidemiology Unit (CAPE), Organization for African Unity. http://www.eldis.org/fulltext/cape_new/MCF_Maasai_Tanzania.pdf
  10. Ellis, J. A., O'Toole, D. T., Haven, T. R. & Davis, W. C. ( 1992; ). Predominance of BoCD8-positive T lymphocytes in vascular lesions in a 1-year-old cow with concurrent malignant catarrhal fever and bovine viral diarrhea virus infection. Vet Pathol 29, 545–547.[CrossRef]
    [Google Scholar]
  11. Ensser, A., Pflanz, R. & Fleckenstein, B. ( 1997; ). Primary structure of the alcelaphine herpesvirus 1 genome. J Virol 71, 6517–6525.
    [Google Scholar]
  12. Gillet, L., Daix, V., Donofrio, G., Wagner, M., Koszinowski, U. H., China, B., Ackermann, M., Markine-Goriaynoff, N. & Vanderplasschen, A. ( 2005; ). Development of bovine herpesvirus 4 as an expression vector using bacterial artificial chromosome cloning. J Gen Virol 86, 907–917.[CrossRef]
    [Google Scholar]
  13. Handley, J. A., Sargan, D. R., Herring, A. J. & Reid, H. W. ( 1995; ). Identification of a region of the alcelaphine herpesvirus-1 genome associated with virulence for rabbits. Vet Microbiol 47, 167–181.[CrossRef]
    [Google Scholar]
  14. Li, H., Shen, D. T., Knowles, D. P., Gorham, J. R. & Crawford, T. B. ( 1994; ). Competitive inhibition enzyme-linked immunosorbent assay for antibody in sheep and other ruminants to a conserved epitope of malignant catarrhal fever virus. J Clin Microbiol 32, 1674–1679.
    [Google Scholar]
  15. Li, H., Dyer, N., Keller, J. & Crawford, T. B. ( 2000; ). Newly recognized herpesvirus causing malignant catarrhal fever in white-tailed deer (Odocoileus virginianus). J Clin Microbiol 38, 1313–1318.
    [Google Scholar]
  16. Mahony, T. J., McCarthy, F. M., Gravel, J. L., West, L. & Young, P. L. ( 2002; ). Construction and manipulation of an infectious clone of the bovine herpesvirus 1 genome maintained as a bacterial artificial chromosome. J Virol 76, 6660–6668.[CrossRef]
    [Google Scholar]
  17. Markine-Goriaynoff, N., Georgin, J.-P., Goltz, M., Zimmermann, W., Broll, H., Wamwayi, H. M., Pastoret, P.-P., Sharp, P. M. & Vanderplasschen, A. ( 2003; ). The core 2 β-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo. J Virol 77, 1784–1792.[CrossRef]
    [Google Scholar]
  18. Messerle, M., Crnkovic, I., Hammerschmidt, W., Ziegler, H. & Koszinowski, U. H. ( 1997; ). Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome. Proc Natl Acad Sci U S A 94, 14759–14763.[CrossRef]
    [Google Scholar]
  19. Morgan, R. W., Cantello, J. L. & McDermott, C. H. ( 1990; ). Transfection of chicken embryo fibroblasts with Marek's disease virus DNA. Avian Dis 34, 345–351.[CrossRef]
    [Google Scholar]
  20. Nakajima, Y., Momotani, E., Ishikawa, Y., Murakami, T., Shimura, N. & Onuma, M. ( 1992; ). Phenotyping of lymphocyte subsets in the vascular and epithelial lesions of a cow with malignant catarrhal fever. Vet Immunol Immunopathol 33, 279–284.[CrossRef]
    [Google Scholar]
  21. Nakajima, Y., Ishikawa, Y., Kadota, K., Kodama, M. & Honma, Y. ( 1994; ). Surface marker analysis of the vascular and epithelia lesions in cattle with sheep-associated malignant catarrhal fever. J Vet Med Sci 56, 1065–1068.[CrossRef]
    [Google Scholar]
  22. Plowright, W. ( 1990; ). Malignant catarrhal fever virus. In Virus Infections of Ruminants, pp. 123–150. Edited by Z. Dinter & B. Morein. Amsterdam: Elsevier.
  23. Plowright, W., Ferris, R. D. & Scott, G. R. ( 1960; ). Blue wildebeest and the aetiological agent of bovine malignant catarrhal fever. Nature 188, 1167–1169.[CrossRef]
    [Google Scholar]
  24. Plowright, W., Herniman, K. A., Jessett, D. M., Kalunda, M. & Rampton, C. S. ( 1975; ). Immunisation of cattle against the herpesvirus of malignant catarrhal fever: failure of inactivated culture vaccines with adjuvant. Res Vet Sci 19, 159–166.
    [Google Scholar]
  25. Reid, H. W. ( 2000; ). Malignant catarrhal fever. Infect Dis Rev 2, 20–22.
    [Google Scholar]
  26. Reid, H. W., Buxton, D., Berrie, E., Pow, I. & Finlayson, J. ( 1984; ). Malignant catarrhal fever. Vet Rec 114, 581–583.[CrossRef]
    [Google Scholar]
  27. Smith, G. A. & Enquist, L. W. ( 2000; ). A self-recombining bacterial artificial chromosome and its application for analysis of herpesvirus pathogenesis. Proc Natl Acad Sci U S A 97, 4873–4878.[CrossRef]
    [Google Scholar]
  28. Stavropoulos, T. A. & Strathdee, C. A. ( 1998; ). An enhanced packaging system for helper-dependent herpes simplex virus vectors. J Virol 72, 7137–7143.
    [Google Scholar]
  29. Swa, S., Wright, H., Thomson, J., Reid, H. & Haig, D. ( 2001; ). Constitutive activation of Lck and Fyn tyrosine kinases in large granular lymphocytes infected with the γ-herpesvirus agents of malignant catarrhal fever. Immunology 102, 44–52.[CrossRef]
    [Google Scholar]
  30. Vanderplasschen, A., Bublot, M., Dubuisson, J., Pastoret, P.-P. & Thiry, E. ( 1993; ). Attachment of the gammaherpesvirus bovine herpesvirus 4 is mediated by the interaction of gp8 glycoprotein with heparinlike moieties on the cell surface. Virology 196, 232–240.[CrossRef]
    [Google Scholar]
  31. Vanderplasschen, A., Markine-Goriaynoff, N., Lomonte, P. & 8 other authors ( 2000; ). A multipotential β-1,6-N-acetylglucosaminyl-transferase is encoded by bovine herpesvirus type 4. Proc Natl Acad Sci U S A 97, 5756–5761.[CrossRef]
    [Google Scholar]
  32. Wagner, M., Ruzsics, Z. & Koszinowski, U. H. ( 2002; ). Herpesvirus genetics has come of age. Trends Microbiol 10, 318–324.[CrossRef]
    [Google Scholar]
  33. Wilkinson, J. M., Galea-Lauri, J. & Reid, H. W. ( 1992; ). A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens. Immunology 77, 106–108.
    [Google Scholar]
  34. Wright, H., Stewart, J. P., Ireri, R. G., Campbell, I., Pow, I., Reid, H. W. & Haig, D. M. ( 2003; ). Genome re-arrangements associated with loss of pathogenicity of the gamma-herpesvirus alcelaphine herpesvirus-1. Res Vet Sci 75, 163–168.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.81465-0
Loading
/content/journal/jgv/10.1099/vir.0.81465-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error