1887

Abstract

Placentae from scrapie-infected ewes have been shown to accumulate PrP when the genotype of the fetus is of a susceptible genotype (VRQ/VRQ, ARQ/VRQ or ARQ/ARQ). Cotyledons from fetuses of genotypes ARR/ARR, ARQ/ARR and ARQ/VRR have previously been shown to be resistant to PrP accumulation. By using ewes from a naturally infected scrapie flock, cotyledons from fetuses of multiple births of different genotypes were examined. PrP was detected in fetal cotyledons of genotype ARQ/ARQ, but not in cotyledons from their dizygotic twin of genotype ARQ/ARR. This confirms earlier reports of single fetuses of these genotypes, but is the first description of such a finding in twin fetuses, one of each genotype. It is also demonstrated that cotyledons from sibling fetuses of genotypes ARQ/VRQ and ARQ/ARQ have different patterns of PrP accumulation depending on whether the dam is of genotype ARQ/ARQ or ARQ/VRQ. Lastly, it is shown that cotyledons from fetuses with resistant genotypes are weakly positive for PrP when they have shared the same pregnant uterine horn with a fetus of a susceptible genotype with cotyledons positive for the detection of PrP. Additionally, a PCR product for the gene, a product specific to males, was found in cotyledons from female fetuses that had shared a uterine horn with a male fetus. This indicates that some sharing of fetal blood occurs between placentomes and fetuses residing in the same uterine horn, which can result in PrP accumulation in cotyledons with resistant genotypes.

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2006-04-01
2019-11-18
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References

  1. Andréoletti, O., Berthon, P., Levavasseur, E., Marc, D., Lantier, F., Monks, E., Elsen, J.-M. & Schelcher, F. ( 2002a; ). Phenotyping of protein–prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry. J Histochem Cytochem 50, 1357–1370.[CrossRef]
    [Google Scholar]
  2. Andréoletti, O., Lacroux, C., Chabert, A. & 8 other authors ( 2002b; ). PrPSc accumulation in placentas of ewes exposed to natural scrapie: influence of foetal PrP genotype and effect on ewe-to-lamb transmission. J Gen Virol 83, 2607–2616.
    [Google Scholar]
  3. Belt, P. B. G. M., Muileman, I. H., Schreuder, B. E. C., Bos-de Ruijter, J., Gielkens, A. L. J. & Smits, M. A. ( 1995; ). Identification of five allelic variants of the sheep PrP gene and their association with natural scrapie. J Gen Virol 76, 509–517.[CrossRef]
    [Google Scholar]
  4. Buschmann, A., Lühken, G., Schultz, J., Erhardt, G. & Groschup, M. H. ( 2004; ). Neuronal accumulation of abnormal prion protein in sheep carrying a scrapie-resistant genotype (PrPARR/ARR). J Gen Virol 85, 2727–2733.[CrossRef]
    [Google Scholar]
  5. Caplazi, P., O'Rourke, K., Wolf, C., Shaw, D. & Baszler, T. V. ( 2004; ). Biology of PrPSc accumulation in two natural scrapie-infected sheep flocks. J Vet Diagn Invest 16, 489–496.[CrossRef]
    [Google Scholar]
  6. Clouscard, C., Beaudry, P., Elsen, J. M. & 7 other authors ( 1995; ). Different allelic effects of the codons 136 and 171 of the prion protein gene in sheep with natural scrapie. J Gen Virol 76, 2097–2101.[CrossRef]
    [Google Scholar]
  7. Evoniuk, J. M., Stoltenow, C. L., O'Rourke, K. I., Moore, B. L. & Redmer, D. A. ( 2005; ). Assessment of the genetic risk and impact of lateral transmission in a valine-associated scrapie outbreak in sheep. Am J Vet Res 66, 1302–1307.[CrossRef]
    [Google Scholar]
  8. Goldmann, W., Hunter, N., Benson, G., Foster, J. D. & Hope, J. ( 1991; ). Different scrapie-associated fibril proteins (PrP) are encoded by lines of sheep selected for different alleles of the Sip gene. J Gen Virol 72, 2411–2417.[CrossRef]
    [Google Scholar]
  9. Griffiths, R. & Tiwari, B. ( 1993; ). The isolation of molecular genetic markers for the identification of sex. Proc Natl Acad Sci U S A 90, 8324–8326.[CrossRef]
    [Google Scholar]
  10. Hunter, N., Foster, J. D., Benson, G. & Hope, J. ( 1991; ). Restriction fragment length polymorphisms of the scrapie-associated fibril protein (PrP) gene and their association with susceptibility to natural scrapie in British sheep. J Gen Virol 72, 1287–1292.[CrossRef]
    [Google Scholar]
  11. Hunter, N., Goldmann, W., Smith, G. & Hope, J. ( 1994; ). The association of a codon 136 PrP gene variant with the occurrence of natural scrapie. Arch Virol 137, 171–177.[CrossRef]
    [Google Scholar]
  12. Hunter, N., Foster, J. D., Goldmann, W., Stear, M. J., Hope, J. & Bostock, C. ( 1996; ). Natural scrapie in a closed flock of Cheviot sheep occurs only in specific PrP genotypes. Arch Virol 141, 809–824.[CrossRef]
    [Google Scholar]
  13. Hunter, N., Goldmann, W., Foster, J. D., Cairns, D. & Smith, G. ( 1997a; ). Natural scrapie and PrP genotype: case–control studies in British sheep. Vet Rec 141, 137–140.[CrossRef]
    [Google Scholar]
  14. Hunter, N., Moore, L., Hosie, B. D., Dingwall, W. S. & Greig, A. ( 1997b; ). Association between natural scrapie and PrP genotype in a flock of Suffolk sheep in Scotland. Vet Rec 140, 59–63.[CrossRef]
    [Google Scholar]
  15. Ikeda, T., Horiuchi, M., Ishiguro, N., Muramatsu, Y., Kai-Uwe, G. D. & Shinagawa, M. ( 1995; ). Amino acid polymorphisms of PrP with reference to onset of scrapie in Suffolk and Corriedale sheep in Japan. J Gen Virol 76, 2577–2581.[CrossRef]
    [Google Scholar]
  16. Ikegami, Y., Ito, M., Isomura, H., Momotani, E., Sasaki, K., Muramatsu, Y., Ishiguro, N. & Shinagawa, M. ( 1991; ). Pre-clinical and clinical diagnosis of scrapie by detection of PrP protein in tissues of sheep. Vet Rec 128, 271–275.[CrossRef]
    [Google Scholar]
  17. Koopman, P., Gubbay, J., Vivian, N., Goodfellow, P. & Lovell-Badge, R. ( 1991; ). Male development of chromosomally female mice transgenic for Sry. Nature 351, 117–121.[CrossRef]
    [Google Scholar]
  18. Laplanche, J. L., Chatelain, J., Westaway, D., Thomas, S., Dussaucy, M., Brugere-Picoux, J. & Launay, J. M. ( 1993; ). PrP polymorphisms associated with natural scrapie discovered by denaturing gradient gel electrophoresis. Genomics 15, 30–37.[CrossRef]
    [Google Scholar]
  19. Madec, J.-Y., Simon, S., Lezmi, S., Bencsik, A., Grassi, J. & Baron, T. ( 2004; ). Abnormal prion protein in genetically resistant sheep from a scrapie-infected flock. J Gen Virol 85, 3483–3486.[CrossRef]
    [Google Scholar]
  20. Onodera, T., Ikeda, T., Muramatsu, Y. & Shinagawa, M. ( 1993; ). Isolation of scrapie agent from the placenta of sheep with natural scrapie in Japan. Microbiol Immunol 37, 311–316.[CrossRef]
    [Google Scholar]
  21. O'Rourke, K. I., Baszler, T. V., Besser, T. E. & 9 other authors ( 2000; ). Preclinical diagnosis of scrapie by immunohistochemistry of third eyelid lymphoid tissue. J Clin Microbiol 38, 3254–3259.
    [Google Scholar]
  22. Pattison, I. H., Hoare, M. N., Jebbett, J. N. & Watson, W. A. ( 1972; ). Spread of scrapie to sheep and goats by oral dosing with foetal membranes from scrapie-affected sheep. Vet Rec 90, 465–468.[CrossRef]
    [Google Scholar]
  23. Payen, E. J. & Cotinot, C. Y. ( 1993; ). Comparative HMG-box sequences of the SRY gene between sheep, cattle and goats. Nucleic Acids Res 21, 2772.[CrossRef]
    [Google Scholar]
  24. Race, R., Jenny, A. & Sutton, D. ( 1998; ). Scrapie infectivity and protease K-resistant prion protein in sheep placenta, brain, spleen, and lymph node: implications for transmission and antemortem diagnosis. J Infect Dis 178, 949–953.[CrossRef]
    [Google Scholar]
  25. Spraker, T. R., Balachandran, A., Zhuang, D. & O'Rourke, K. I. ( 2004; ). Variable patterns of distribution of PrPCWD in the obex and cranial lymphoid tissues of Rocky Mountain elk (Cervus elaphus nelsoni) with subclinical chronic wasting disease. Vet Rec 155, 295–302.[CrossRef]
    [Google Scholar]
  26. Szatkowska, I., Zych, S., Udała, J., Dybus, A., Błaszczyk, P., Sysa, P. & Da̧browski, T. ( 2004; ). Freemartinism: three cases in goats. Acta Vet Brno 73, 375–378.[CrossRef]
    [Google Scholar]
  27. Tuo, W., Zhuang, D., Knowles, D. P., Cheevers, W. P., Sy, M.-S. & O'Rourke, K. I. ( 2001; ). PrP-C and PrP-Sc at the fetal-maternal interface. J Biol Chem 276, 18229–18234.[CrossRef]
    [Google Scholar]
  28. Tuo, W., O'Rourke, K. I., Zhuang, D., Cheevers, W. P., Spraker, T. R. & Knowles, D. P. ( 2002; ). Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep. Proc Natl Acad Sci U S A 99, 6310–6315.[CrossRef]
    [Google Scholar]
  29. Wilson, P. J. & White, B. N. ( 1998; ). Sex identification of elk (Cervus elaphus canadensis), moose (Alces alces), and white-tailed deer (Odocoileus virginianus) using the polymerase chain reaction. J Forensic Sci 43, 477–482.
    [Google Scholar]
  30. Wooding, F. B. P., Morgan, G. & Adam, C. L. ( 1997; ). Structure and function in the ruminant synepitheliochorial placenta: central role of the trophoblast binucleate cell in deer. Microsc Res Tech 38, 88–99.[CrossRef]
    [Google Scholar]
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