1887

Abstract

The placenta is unique amongst normal tissues in transcribing numerous different human endogenous retroviruses at high levels. In this study, RT-PCR and immunohistochemistry were used to investigate the expression of syncytin in human trophoblast. Syncytin transcripts were found in first-trimester trophoblast cells with both villous and extravillous phenotypes and also in the JAR and JEG-3 choriocarcinoma cell lines. Syncytin protein was detected in villous trophoblast and in all extravillous trophoblast subpopulations of first- and second-trimester placental tissues. It was also present in ectopic trophoblast from tubal implantations. This study confirms that syncytin is expressed widely by a variety of normal human trophoblast populations, as well as choriocarcinoma cell lines.

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2006-07-01
2020-10-23
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References

  1. Al-Lamki R. S., Skepper J. N., Burton G. J. 1999; Are human placental bed giant cells merely aggregates of small mononuclear trophoblast cells? An ultrastructural and immunocytochemical study. Hum Reprod 14:496–504 [CrossRef]
    [Google Scholar]
  2. Bénit L., Dessen P., Heidmann T. 2001; Identification, phylogeny, and evolution of retroviral elements based on their envelope genes. J Virol 75:11709–11719 [CrossRef]
    [Google Scholar]
  3. Blaise S., de Parseval N., Bénit L., Heidmann T. 2003; Genomewide screening for fusogenic human endogenous retrovirus envelopes identifies syncytin 2, a gene conserved on primate evolution. Proc Natl Acad Sci U S A 100:13013–13018 [CrossRef]
    [Google Scholar]
  4. Blond J.-L., Besème F., Duret L., Bouton O., Bedin F., Perron H., Mandrand B., Mallet F. 1999; Molecular characterization and placental expression of HERV-W, a new human endogenous retrovirus family. J Virol 73:1175–1185
    [Google Scholar]
  5. Blond J.-L., LaVillette D., Cheynet V., Bouton O., Oriol G., Chapel-Fernandes S., Mandrand B., Mallet F., Cosset F.-L. 2000; An envelope glycoprotein of the human endogenous retrovirus HERV-W is expressed in the human placenta and fuses cells expressing the type D mammalian retrovirus receptor. J Virol 74:3321–3329 [CrossRef]
    [Google Scholar]
  6. Burrows T. D., King A., Loke Y. W. 1993; Expression of integrins by human trophoblast and differential adhesion to laminin or fibronectin. Hum Reprod 8:475–484
    [Google Scholar]
  7. Chumbley G., Hawley S., Carter N. P., Loke Y. W. 1991; Human extravillous trophoblast MHC class I expression is resistant to regulation by interferon- α . J Reprod Immunol 20:289–296 [CrossRef]
    [Google Scholar]
  8. Chumbley G., King A., Gardner L., Howlett S., Holmes N., Loke Y. W. 1994; Generation of an antibody to HLA-G in transgenic mice and demonstration of the tissue reactivity of this antibody. J Reprod Immunol 27:173–186 [CrossRef]
    [Google Scholar]
  9. Cianciolo G., Copeland T. D., Oroszlan S., Snyderman R. 1985; Inhibition of lymphocyte proliferation by a synthetic peptide homologous to retroviral envelope proteins. Science 230:453–455 [CrossRef]
    [Google Scholar]
  10. de Parseval N., Lazar V., Casella J.-F., Benit L., Heidmann T. 2003; Survey of human genes of retroviral origin: identification and transcriptome of the genes with coding capacity for complete envelope proteins. J Virol 77:10414–10422 [CrossRef]
    [Google Scholar]
  11. Frendo J.-L., Olivier D., Cheynet V., Blond J.-L., Bouton O., Vidaud M., Rabreau M., Evain-Brion D., Mallet F. 2003; Direct involvement of HERV-W Env glycoprotein in human trophoblast cell fusion and differentiation. Mol Cell Biol 23:3566–3574 [CrossRef]
    [Google Scholar]
  12. Harris D. T., Cianciolo G. J., Snyderman R., Argov S., Koren H. S. 1987; Inhibition of human natural killer cell activity by a synthetic peptide homologous to a conserved region in the retroviral protein, p15E. J Immunol 138:889–894
    [Google Scholar]
  13. International Human Genome Sequencing Consortium; 2001; Initial sequencing and analysis of the human genome. Nature 409:860–921 [CrossRef]
    [Google Scholar]
  14. Knerr I., Beinder E., Rascher W. 2002; Syncytin, a novel human endogenous retroviral gene in human placenta: evidence for its dysregulation in preeclampsia and HELLP syndrome. Am J Obstet Gynecol 186:210–213 [CrossRef]
    [Google Scholar]
  15. Lee X., Keith J. C. Jr, Stumm N. & 9 other authors 2001; Downregulation of placental syncytin expression and abnormal protein localization in pre-eclampsia. Placenta 22:808–812 [CrossRef]
    [Google Scholar]
  16. Loke Y. W., Burland K. 1988; Human trophoblast cells cultured in modified medium and supported by extracellular matrix. Placenta 9:173–182 [CrossRef]
    [Google Scholar]
  17. Loke Y. W., King A. 1995 Human Implantation: Cell Biology and Immunology Cambridge: Cambridge University Press;
    [Google Scholar]
  18. Loke Y. W., Hsi B.-L., Bulmer J., Grivaux C., Hawley S., Gardner L., King A., Carter N. P. 1992; Evaluation of a monoclonal antibody, BC-1, which identifies an antigen expressed on the surface membrane of human extravillous trophoblast. Am J Reprod Immunol 27:77–81 [CrossRef]
    [Google Scholar]
  19. Loke Y. W., King A., Burrows T., Gardner L., Bowen M., Hiby S., Howlett S., Holmes N., Jacobs D. 1997; Evaluation of trophoblast HLA-G antigen with a specific monoclonal antibody. Tissue Antigens 50:135–146 [CrossRef]
    [Google Scholar]
  20. Malassiné A., Handschuh K., Tsatsaris V., Gerbaud P., Cheynet V., Oriol G., Mallet F., Evain-Brion D. 2005; Expression of HERV-W Env glycoprotein (syncytin) in the extravillous trophoblast of first trimester human placenta. Placenta 26:556–562 [CrossRef]
    [Google Scholar]
  21. Mi S., Lee X., Li X.-P. & 9 other authors 2000; Syncytin is a captive retroviral envelope protein involved in human placental morphogenesis. Nature 403:785–789 [CrossRef]
    [Google Scholar]
  22. Moffett-King A. 2002; Natural killer cells and pregnancy. Nat Rev Immunol 2:656–663 [CrossRef]
    [Google Scholar]
  23. Muir A., Lever A., Moffett A. 2004; Expression and functions of human endogenous retroviruses in the placenta: an update. Placenta 25 (Suppl. A):S16–S25 [CrossRef]
    [Google Scholar]
  24. Nakagawa K., Harrison L. C. 1996; The potential roles of endogenous retroviruses in autoimmunity. Immunol Rev 152:193–236 [CrossRef]
    [Google Scholar]
  25. Okahara G., Matsubara S., Oda T., Sugimoto J., Jinno Y., Kanaya F. 2004; Expression analyses of human endogenous retroviruses (HERVs): tissue-specific and developmental stage-dependent expression of HERVs. Genomics 84:982–990 [CrossRef]
    [Google Scholar]
  26. Pötgens A. J. G., Drewlo S., Kokozidou M., Kaufmann P. 2004; Syncytin: the major regulator of trophoblast fusion? Recent developments and hypotheses on its action. Hum Reprod Update 10:487–496 [CrossRef]
    [Google Scholar]
  27. Smallwood A., Papageorghiou A., Nicolaides K., Alley M. K. R., Jim A., Nargund G., Ojha K., Campbell S., Banerjee S. 2003; Temporal regulation of the expression of syncytin ( HERV-W ), maternally imprinted PEG10 , and SGCE in human placenta. Biol Reprod 69:286–293 [CrossRef]
    [Google Scholar]
  28. Stoye J. P., Coffin J. M. 2000; A provirus put to work. Nature 403:715–717 [CrossRef]
    [Google Scholar]
  29. Taruscio D., Mantovani A. 1998; Human endogenous retroviral sequences: possible roles in reproductive physiopathology. Biol Reprod 59:713–724 [CrossRef]
    [Google Scholar]
  30. Voisset C., Bouton O., Bedin F., Duret L., Mandrand B., Mallet F., Paranhos-Baccalà G. 2000; Chromosomal distribution and coding capacity of the human endogenous retrovirus HERV-W family. AIDS Res Hum Retroviruses 16:731–740 [CrossRef]
    [Google Scholar]
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