@article{mbs:/content/journal/jgv/10.1099/vir.0.81396-0, author = "Bazzini, A. A. and Asurmendi, S. and Hopp, H. E. and Beachy, R. N.", title = "Tobacco mosaic virus (TMV) and potato virus X (PVX) coat proteins confer heterologous interference to PVX and TMV infection, respectively", journal= "Journal of General Virology", year = "2006", volume = "87", number = "4", pages = "1005-1012", doi = "https://doi.org/10.1099/vir.0.81396-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81396-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Replication of Potato virus X (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CPWT) of Tobacco mosaic virus (TMV) or a mutant CP, CPT42W, that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and did not produce pseudovirions, CPT28W, did not reduce PVX replication. Similarly, in transgenic tobacco plants producing TMV CPWT or CPT42W, there was a delay in local cell-to-cell spread of PVX infection that was not observed in CPT28W plants or in non-transgenic plants. The results suggest that the quaternary structure of the TMV CP regulates the mechanism(s) of heterologous CP-mediated resistance. Similarly, transgenic protoplasts that produced PVX CP conferred transient protection against infection by TMV RNA. Transgenic plants that accumulated PVX CP reduced the cell-to-cell spread of infection and resulted in a delay in systemic infection following inoculation with TMV or TMV RNA. Heterologous CP-mediated resistance was characterized by a brief delay in systemic infection, whilst homologous CP-mediated resistance conferred reduced or no systemic infection.", }