1887

Abstract

Replication of (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CP) of (TMV) or a mutant CP, CP, that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and did not produce pseudovirions, CP, did not reduce PVX replication. Similarly, in transgenic tobacco plants producing TMV CP or CP, there was a delay in local cell-to-cell spread of PVX infection that was not observed in CP plants or in non-transgenic plants. The results suggest that the quaternary structure of the TMV CP regulates the mechanism(s) of heterologous CP-mediated resistance. Similarly, transgenic protoplasts that produced PVX CP conferred transient protection against infection by TMV RNA. Transgenic plants that accumulated PVX CP reduced the cell-to-cell spread of infection and resulted in a delay in systemic infection following inoculation with TMV or TMV RNA. Heterologous CP-mediated resistance was characterized by a brief delay in systemic infection, whilst homologous CP-mediated resistance conferred reduced or no systemic infection.

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2006-04-01
2020-10-31
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