1887

Abstract

Replication of (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CP) of (TMV) or a mutant CP, CP, that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and did not produce pseudovirions, CP, did not reduce PVX replication. Similarly, in transgenic tobacco plants producing TMV CP or CP, there was a delay in local cell-to-cell spread of PVX infection that was not observed in CP plants or in non-transgenic plants. The results suggest that the quaternary structure of the TMV CP regulates the mechanism(s) of heterologous CP-mediated resistance. Similarly, transgenic protoplasts that produced PVX CP conferred transient protection against infection by TMV RNA. Transgenic plants that accumulated PVX CP reduced the cell-to-cell spread of infection and resulted in a delay in systemic infection following inoculation with TMV or TMV RNA. Heterologous CP-mediated resistance was characterized by a brief delay in systemic infection, whilst homologous CP-mediated resistance conferred reduced or no systemic infection.

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2006-04-01
2019-11-16
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References

  1. Abel, P. P., Nelson, R. S., De, B., Hoffmann, N., Rogers, S. G., Fraley, R. T. & Beachy, R. N. ( 1986; ). Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232, 738–743.[CrossRef]
    [Google Scholar]
  2. Anderson, E. J., Stark, D. M., Nelson, R. S., Tumer, N. E. & Beachy, R. N. ( 1989; ). Transgenic plants that express the coat protein genes of tobacco mosaic virus or alfalfa mosaic virus interfere with disease development of some nonrelated viruses. Phytopathology 79, 1284–1290.[CrossRef]
    [Google Scholar]
  3. Asurmendi, S., Berg, R. H., Koo, J. C. & Beachy, R. N. ( 2004; ). Coat protein regulates formation of replication complexes during tobacco mosaic virus infection. Proc Natl Acad Sci U S A 101, 1415–1420.[CrossRef]
    [Google Scholar]
  4. Batten, J. S., Yoshinari, S. & Hemenway, C. ( 2003; ). Potato virus X: a model system for virus replication, movement and gene expression. Mol Plant Pathol 4, 125–131.[CrossRef]
    [Google Scholar]
  5. Beachy, R. N. ( 1999; ). Coat-protein-mediated resistance to tobacco mosaic virus: discovery mechanisms and exploitation. Philos Trans R Soc Lond B Biol Sci 354, 659–664.[CrossRef]
    [Google Scholar]
  6. Bendahmane, M. & Beachy, R. N. ( 1999; ). Control of tobamovirus infections via pathogen-derived resistance. Adv Virus Res 53, 369–386.
    [Google Scholar]
  7. Bendahmane, M., Fitchen, J. H., Zhang, G. & Beachy, R. N. ( 1997; ). Studies of coat protein-mediated resistance to tobacco mosaic tobamovirus: correlation between assembly of mutant coat proteins and resistance. J Virol 71, 7942–7950.
    [Google Scholar]
  8. Bendahmane, M., Szécsi, J., Chen, I., Berg, R. H. & Beachy, R. N. ( 2002; ). Characterization of mutant tobacco mosaic virus coat protein that interferes with virus cell-to-cell movement. Proc Natl Acad Sci U S A 99, 3645–3650.[CrossRef]
    [Google Scholar]
  9. Carette, J. E., Stuiver, M., Van Lent, J., Wellink, J. & Van Kammen, A. ( 2000; ). Cowpea mosaic virus infection induces a massive proliferation of endoplasmic reticulum but not Golgi membranes and is dependent on de novo membrane synthesis. J Virol 74, 6556–6563.[CrossRef]
    [Google Scholar]
  10. Carr, J. P., Beachy, R. N. & Klessig, D. F. ( 1989; ). Are the PR1 proteins of tobacco involved in genetically engineered resistance to TMV? Virology 169, 470–473.[CrossRef]
    [Google Scholar]
  11. Chapman, S., Kavanagh, T. & Baulcombe, D. ( 1992; ). Potato virus X as a vector for gene expression in plants. Plant J 2, 549–557.
    [Google Scholar]
  12. Culver, J. N. ( 1996; ). Tobamovirus cross protection using a potexvirus vector. Virology 226, 228–235.[CrossRef]
    [Google Scholar]
  13. Cuozzo, M., O'Connell, K. M., Kaniewski, W., Fang, R.-X., Chua, N.-H. & Tumer, N. E. ( 1988; ). Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNA. Biotechnology 6, 549–557.[CrossRef]
    [Google Scholar]
  14. Deom, C. M., Schubert, K. R., Wolf, S., Holt, C. A., Lucas, W. J. & Beachy, R. N. ( 1990; ). Molecular characterization and biological function of the movement protein of tobacco mosaic virus in transgenic plants. Proc Natl Acad Sci U S A 87, 3284–3288.[CrossRef]
    [Google Scholar]
  15. Fritsch, C., Stussi, C., Witz, J. & Hirth, L. ( 1973; ). Specificity of TMV RNA encapsidation: in vitro coating of heterologous RNA by TMV protein. Virology 56, 33–45.[CrossRef]
    [Google Scholar]
  16. Hemenway, C., Fang, R.-X., Kaniewski, W. K., Chua, N.-H. & Tumer, N. E. ( 1988; ). Analysis of the mechanism of protection in transgenic plants expressing the potato virus X coat protein or its antisense RNA. EMBO J 7, 1273–1280.
    [Google Scholar]
  17. Howard, A. R., Heppler, M. L., Ju, H.-J., Krishnamurthy, K., Payton, M. E. & Verchot-Lubicz, J. ( 2004; ). Potato virus X TGBp1 induces plasmodesmata gating and moves between cells in several host species whereas CP moves only in N. benthamiana leaves. Virology 328, 185–197.[CrossRef]
    [Google Scholar]
  18. Kawakami, S., Watanabe, Y. & Beachy, R. N. ( 2004; ). Tobacco mosaic virus infection spreads cell to cell as intact replication complexes. Proc Natl Acad Sci U S A 101, 6291–6296.[CrossRef]
    [Google Scholar]
  19. Klug, A. ( 1999; ). The tobacco mosaic virus particle: structure and assembly. Philos Trans R Soc Lond B Biol Sci 354, 531–535.[CrossRef]
    [Google Scholar]
  20. Krishnamurthy, K., Heppler, M., Mitra, R., Blancaflor, E., Payton, M., Nelson, R. S. & Verchot-Lubicz, J. ( 2003; ). The Potato virus X TGBp3 protein associates with the ER network for virus cell-to-cell movement. Virology 309, 135–151.[CrossRef]
    [Google Scholar]
  21. Loesch-Fries, L. S., Merlo, D., Zinnen, T., Burhop, L., Hill, K., Krahn, K., Jarvis, N., Nelson, S. & Halk, E. ( 1987; ). Expression of alfalfa mosaic virus RNA 4 in transgenic plants confers virus resistance. EMBO J 6, 1845–1851.
    [Google Scholar]
  22. Lu, B., Stubbs, G. & Culver, J. N. ( 1998; ). Coat protein interactions involved in tobacco mosaic tobamovirus cross-protection. Virology 248, 188–198.[CrossRef]
    [Google Scholar]
  23. Más, P. & Beachy, R. N. ( 1999; ). Replication of tobacco mosaic virus on endoplasmic reticulum and role of the cytoskeleton and virus movement protein in intracellular distribution of viral RNA. J Cell Biol 147, 945–958.[CrossRef]
    [Google Scholar]
  24. Mitra, R., Krishnamurthy, K., Blancaflor, E., Payton, M., Nelson, R. S. & Verchot-Lubicz, J. ( 2003; ). The Potato virus X TGBp2 protein association with the endoplasmic reticulum plays a role in but is not sufficient for viral cell-to-cell movement. Virology 312, 35–48.[CrossRef]
    [Google Scholar]
  25. Nejidat, A. & Beachy, R. N. ( 1990; ). Transgenic tobacco plants expressing a coat protein gene of tobacco mosaic virus are resistant to some other tobamoviruses. Mol Plant Microbe Interact 3, 247–251.[CrossRef]
    [Google Scholar]
  26. Otsuki, Y., Takebe, I., Honda, Y. & Matsui, C. ( 1972; ). Ultrastructure of infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Virology 49, 188–194.[CrossRef]
    [Google Scholar]
  27. Register, J. C., III & Beachy, R. N. ( 1988; ). Resistance to TMV in transgenic plants results from interference with an early event in infection. Virology 166, 524–532.[CrossRef]
    [Google Scholar]
  28. Reichel, C. & Beachy, R. N. ( 1998; ). Tobacco mosaic virus infection induces severe morphological changes of the endoplasmic reticulum. Proc Natl Acad Sci U S A 95, 11169–11174.[CrossRef]
    [Google Scholar]
  29. Sleat, D. E., Gallie, D. R., Watts, J. W., Deom, C. M., Turner, P. C., Beachy, R. N. & Wilson, T. M. A. ( 1988; ). Selective recovery of foreign gene transcripts as virus-like particles in TMV-infected transgenic tobaccos. Nucleic Acids Res 16, 3127–3140.[CrossRef]
    [Google Scholar]
  30. Spillane, C., Verchot, J., Kavanagh, T. A. & Baulcombe, D. C. ( 1997; ). Concurrent suppression of virus replication and rescue of movement-defective virus in transgenic plants expressing the coat protein of potato virus X. Virology 236, 76–84.[CrossRef]
    [Google Scholar]
  31. van Dun, C. M. P., Bol, J. F. & van Vloten-Doting, L. ( 1987; ). Expression of alfalfa mosaic virus and tobacco rattle virus coat protein genes in transgenic tobacco plants. Virology 159, 299–305.[CrossRef]
    [Google Scholar]
  32. van Dun, C. M. P., Overduin, B., van Vloten-Doting, L. & Bol, J. F. ( 1988; ). Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus coat protein does not cross-protect against alfalfa mosaic virus infection. Virology 164, 383–389.[CrossRef]
    [Google Scholar]
  33. Watanabe, Y., Ohno, T. & Okada, Y. ( 1982; ). Virus multiplication in tobacco protoplasts inoculated with tobacco mosaic virus RNA encapsulated in large unilamellar vesicle liposomes. Virology 120, 478–480.[CrossRef]
    [Google Scholar]
  34. Watanabe, Y., Meshi, T. & Okada, Y. ( 1987; ). Infection of tobacco protoplasts with in vitro transcribed tobacco mosaic virus RNA using an improved electroporation method. FEBS Lett 219, 65–69.[CrossRef]
    [Google Scholar]
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