RT Journal Article SR Electronic(1) A1 Fiedler, Marc A1 Ressler, Sigrun A1 Campo-Fernández, Beatriz A1 Laich, Andreas A1 Jansen, Lars A1 Widschwendter, Andreas A1 Viertler, Hans-Peter A1 Bacher, Nicole A1 Morandell, Dieter A1 Müller-Holzner, Elisabeth A1 Dürst, Matthias A1 Jansen-Dürr, Pidder A1 Zwerschke, WernerYR 2005 T1 Expression of the high-risk human papillomavirus type 18 and 45 E7 oncoproteins in cervical carcinoma biopsies JF Journal of General Virology, VO 86 IS 12 SP 3235 OP 3241 DO https://doi.org/10.1099/vir.0.81390-0 PB Microbiology Society, SN 1465-2099, AB E7 proteins are major oncoproteins of high-risk human papillomaviruses (HPVs), which play a key role in cervical carcinogenesis. These proteins have been shown to immortalize primary human cells. Due to the absence of antibodies with suitable sensitivity and specificity, little is known about expression of the E7 oncoproteins in naturally infected tissues. Recently, high-level expression of the E7 protein of HPV-16, the most prevalent oncogenic HPV type, was demonstrated in cervical carcinomas by immunohistochemistry; however, approximately 15 additional high-risk HPV types are known to be associated with cervical carcinoma. It is unknown whether the E7 oncoproteins of HPV-18 and -45, the second and third most prevalent HPV types, are expressed in cervical cancers. Using antibodies against HPV-18 and -45 E7 proteins, it is shown here for the first time that the HPV-18 and -45 E7 proteins can be detected in cervical carcinoma biopsies. Together with anti-HPV-16 E7 antibodies, this could create the possibility of detecting E7 oncoproteins in approximately 80 % of all cervical cancers., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81390-0