1887

Abstract

Recently, glycoprotein G (gG) of several alphaherpesviruses infecting large herbivores was shown to belong to a new family of chemokine-binding proteins (vCKBPs). In the present study, the function of (FeHV-1) gG as a vCKBP was investigated and the following conclusions were reached: (i) FeHV-1 secreted gG is a high-affinity broad-spectrum vCKBP that binds CC, CXC and C chemokines; (ii) gG is the only vCKBP expressed by FeHV-1 that binds CCL3 and CXCL1; (iii) secreted gG blocks chemokine activity by preventing their interaction with high-affinity cellular receptors; (iv) the membrane-anchored form of gG expressed on the surface of infected cells is also able to bind chemokines; and (v) the vCKBP activity is conserved among different field isolates of FeHV-1. Altogether, these data demonstrate that FeHV-1 gG is a new member of the vCKBP-4 family. Moreover, this study is the first to demonstrate that gG expressed at the surface of FeHV-1-infected cells can also bind chemokines.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81388-0
2005-12-01
2019-11-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/12/3209.html?itemId=/content/journal/jgv/10.1099/vir.0.81388-0&mimeType=html&fmt=ahah

References

  1. Alcami, A. ( 2003; ). Viral mimicry of cytokines, chemokines and their receptors. Nat Rev Immunol 3, 36–50.[CrossRef]
    [Google Scholar]
  2. Baranowski, E., Keil, G., Lyaku, J., Rijsewijk, F. A., van Oirschot, J. T., Pastoret, P. P. & Thiry, E. ( 1996; ). Structural and functional analysis of bovine herpesvirus 1 minor glycoproteins. Vet Microbiol 53, 91–101.[CrossRef]
    [Google Scholar]
  3. Bryant, N. A., Davis-Poynter, N., Vanderplasschen, A. & Alcami, A. ( 2003; ). Glycoprotein G isoforms from some alphaherpesviruses function as broad-spectrum chemokine binding proteins. EMBO J 22, 833–846.[CrossRef]
    [Google Scholar]
  4. Dower, S. K., Kronheim, S. R., March, C. J., Conlon, P. J., Hopp, T. P., Gillis, S. & Urdal, D. L. ( 1985; ). Detection and characterization of high affinity plasma membrane receptors for human interleukin 1. J Exp Med 162, 501–515.[CrossRef]
    [Google Scholar]
  5. Drummer, H. E., Studdert, M. J. & Crabb, B. S. ( 1998; ). Equine herpesvirus-4 glycoprotein G is secreted as a disulphide-linked homodimer and is present as two homodimeric species in the virion. J Gen Virol 79, 1205–1213.
    [Google Scholar]
  6. Gaskell, R. ( 1975; ). Studies on Feline viral rhinotracheitis with particular reference to the carrier state. PhD thesis, University of Bristol, UK.
  7. Lalani, A. S. & McFadden, G. ( 1999; ). Evasion and exploitation of chemokines by viruses. Cytokine Growth Factor Rev 10, 219–233.[CrossRef]
    [Google Scholar]
  8. Markine-Goriaynoff, N., Gillet, L., Karlsen, O. A., Haarr, L., Minner, F., Pastoret, P. P., Fukuda, M. & Vanderplasschen, A. ( 2004; ). The core 2 β-1,6-N-acetylglucosaminyltransferase-M encoded by bovine herpesvirus 4 is not essential for virus replication despite contributing to post-translational modifications of structural proteins. J Gen Virol 85, 355–367.[CrossRef]
    [Google Scholar]
  9. Murphy, P. M. ( 1996; ). Chemokine receptors: structure, function and role in microbial pathogenesis. Cytokine Growth Factor Rev 7, 47–64.[CrossRef]
    [Google Scholar]
  10. Murphy, P. M. ( 2001; ). Viral exploitation and subversion of the immune system through chemokine mimicry. Nat Immunol 2, 116–122.[CrossRef]
    [Google Scholar]
  11. Richman, D. D., Buckmaster, A., Bell, S., Hodgman, C. & Minson, A. C. ( 1986; ). Identification of a new glycoprotein of herpes simplex virus type 1 and genetic mapping of the gene that codes for it. J Virol 57, 647–655.
    [Google Scholar]
  12. Roizman, B. & Pellett, P. E. ( 2001; ). The family Herpesviridae: a brief introduction. In Fields Virology, 4th edn, pp. 2381–2397. Edited by P. M. Howley & D. M. Knipe. Philadelphia: Lippincott Williams & Wilkins.
  13. Stiles, J. ( 2003; ). Feline herpesvirus. Clin Tech Small Anim Pract 18, 178–185.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.81388-0
Loading
/content/journal/jgv/10.1099/vir.0.81388-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 3209 - 3214

Surface plasmon resonance analysis

Real-time binding data of human chemokines to purified soluble gG.

Construction of a recombinant FeHV-1 lacking gG and a derived revertant virus. [Single PDF file](340 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error