1887

Abstract

To clarify the stability of the BK polyomavirus (BKPyV) genome in renal transplant (RT) recipients, three to five complete BKPyV genomes from each of six RT recipients with surviving renal allografts were molecularly cloned. The complete sequences of these clones were determined and compared in each patient. No nucleotide difference was detected among clones in two patients, and a few nucleotide variations were found among those in four patients. In each of these patients a parental sequence (usually the major sequence), from which variant sequences (usually minor sequences) with nucleotide substitutions would have been generated, were identified. A comparison between the parental and variant sequences in each patient identified a single nucleotide substitution in each variant sequence. From these findings, it was concluded that the genome of BKPyV is stable in RT recipients without nephropathy, with only minor nucleotide substitutions in the coding region.

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2006-02-01
2024-04-20
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References

  1. Baksh F. K., Finkelstein S. D., Swalsky P. A., Stoner G. L., Ryschkewitsch C. F., Randhawa P. 2001; Molecular genotyping of BK and JC viruses in human polyomavirus-associated interstitial nephritis after renal transplantation. Am J Kidney Dis 38:354–365 [CrossRef]
    [Google Scholar]
  2. Chang D., Liou Z.-M., Ou W.-C., Wang K.-Z., Wang M., Fung C.-Y., Tsai R.-T. 1996; Production of the antigen and the antibody of the JC virus major capsid protein VP1. J Virol Methods 59:177–187 [CrossRef]
    [Google Scholar]
  3. Chen C.-H., Wen M.-C., Wang M., Lian J.-D., Wu M.-J., Cheng C.-H., Shu K.-H., Chang D. 2001; A regulatory region rearranged BK virus is associated with tubulointerstitial nephritis in a rejected renal allograft. J Med Virol 64:82–88 [CrossRef]
    [Google Scholar]
  4. Chen Y., Sharp P. M., Fowkes M., Kocher O., Joseph J. T., Koralnik I. J. 2004; Analysis of 15 novel full-length BK virus sequences from three individuals: evidence of a high intra-strain genetic diversity. J Gen Virol 85:2651–2663 [CrossRef]
    [Google Scholar]
  5. de Bruyn G., Limaye A. P. 2004; BK virus-associated nephropathy in kidney transplant recipients. Rev Med Virol 14:193–205 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  8. Page R. D. M. 1996; treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  9. Randhawa P. S., Khaleel-Ur-Rehman K., Swalsky P. A., Vats A., Scantlebury V., Shapiro R., Finkelstein S. 2002; DNA sequencing of viral capsid protein VP-1 region in patients with BK virus interstitial nephritis. Transplantation 73:1090–1094 [CrossRef]
    [Google Scholar]
  10. Randhawa P., Zygmunt D., Shapiro R., Vats A., Weck K., Swalsky P., Finkelstein S. 2003; Viral regulatory region sequence variations in kidney tissue obtained from patients with BK virus nephropathy. Kidney Int 64:743–747 [CrossRef]
    [Google Scholar]
  11. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  12. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning, a Laboratory Manual , 2nd edn. Cold Spring Harbor: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Seif I., Khoury G., Dhar R. 1979; The genome of human papovavirus BKV. Cell 18:963–977 [CrossRef]
    [Google Scholar]
  14. Takasaka T., Goya N., Tokumoto T. & 14 other authors 2004; Subtypes of BK virus prevalent in Japan and variation in their transcriptional control region. J Gen Virol 85:2821–2827 [CrossRef]
    [Google Scholar]
  15. Tavis J. E., Walker D. L., Gardner S. D., Frisque R. J. 1989; Nucleotide sequence of the human polyomavirus AS virus, an antigenic variant of BK virus. J Virol 63:901–911
    [Google Scholar]
  16. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  17. Yogo Y., Iida T., Taguchi F., Kitamura T., Aso Y. 1991; Typing of human polyomavirus JC virus on the basis of restriction fragment length polymorphisms. J Clin Microbiol 29:2130–2138
    [Google Scholar]
  18. Zheng H. Y., Kitamura T., Takasaka T., Chen Q., Yogo Y. 2004; Unambiguous identification of JC polyomavirus strains transmitted from parents to children. Arch Virol 149:261–273 [CrossRef]
    [Google Scholar]
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