1887

Abstract

Seven of the eight genes in the avian pneumovirus (APV) genome contain a conserved 9 nt transcriptional start sequence with the virus large (L) polymerase gene differing from the consensus at three positions. The sequence requirements of the APV transcriptional gene start sequence were investigated by generating a series of mutations in which each of the nine conserved bases was mutated to each of the other three possible nucleotides in a minigenome containing two reporter genes. The effect of each mutation was assessed by measuring the relative levels of expression from the altered and unaltered gene start sequences. Mutations at positions 2, 7 and 9 significantly reduced transcription levels while alterations to position 5 had little effect. The L gene start sequence directed transcription at levels approximately 50 % below that of the consensus gene start sequence. These data suggest that there are common features in pneumovirus transcriptional control sequences.

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2005-12-01
2019-11-14
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References

  1. Bennett, R. S., McComb, B., Shin, H. J., Njenga, M. K., Nagaraja, K. V. & Halvorson, D. A. ( 2002; ). Detection of avian pneumovirus in wild Canada (Branta canadensis) and blue-winged teal (Anas discors) geese. Avian Dis 46, 1025–1029.[CrossRef]
    [Google Scholar]
  2. Bennett, R. S., Nezworski, J., Velayudhan, B. T. & 7 other authors ( 2004; ). Evidence of avian pneumovirus spread beyond Minnesota among wild and domestic birds in central North America. Avian Dis 48, 902–908.[CrossRef]
    [Google Scholar]
  3. Buchholz, U. J., Finke, S. & Conzelmann, K. K. ( 1999; ). Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J Virol 73, 251–259.
    [Google Scholar]
  4. Bukreyev, A., Camargo, E. & Collins, P. L. ( 1996; ). Recovery of infectious respiratory syncytial virus expressing an additional, foreign gene. J Virol 70, 6634–6641.
    [Google Scholar]
  5. Cavanagh, D. & Barrett, T. ( 1988; ). Pneumovirus-like characteristics of the mRNA and proteins of turkey rhinotracheitis virus. Virus Res 11, 241–256.[CrossRef]
    [Google Scholar]
  6. Collins, M. S. & Gough, R. E. ( 1988; ). Characterization of a virus associated with turkey rhinotracheitis. J Gen Virol 69, 909–916.[CrossRef]
    [Google Scholar]
  7. Collins, P. L., Dickens, L. E., Buckler-White, A., Olmsted, R. A., Spriggs, M. K., Camargo, E. & Coelingh, K. V. W. ( 1986; ). Nucleotide sequences for gene junctions of human respiratory syncytial virus reveal distinctive features of intergenic structure and gene order. Proc Natl Acad Sci U S A 83, 4594–4598.[CrossRef]
    [Google Scholar]
  8. Collins, P. L., Hill, M. G., Camargo, E., Grosfeld, H., Chanock, R. M. & Murphy, B. R. ( 1995; ). Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5′ proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development. Proc Natl Acad Sci U S A 92, 11563–11567.[CrossRef]
    [Google Scholar]
  9. Dickens, L. E., Collins, P. L. & Wertz, G. W. ( 1984; ). Transcriptional mapping of human respiratory syncytial virus. J Virol 52, 364–369.
    [Google Scholar]
  10. Jacobs, J. A., Njenga, M. K., Alvarez, R., Mawditt, K., Britton, P., Cavanagh, D. & Seal, B. S. ( 2003; ). Subtype B avian metapneumovirus resembles subtype A more closely than subtype C or human metapneumovirus with respect to the phosphoprotein, and second matrix and small hydrophobic proteins. Virus Res 92, 171–178.[CrossRef]
    [Google Scholar]
  11. Jones, R. C. ( 1996; ). Avian pneumovirus infection: questions still unanswered. Avian Pathol 25, 636–648.
    [Google Scholar]
  12. Kozak, M. ( 1986; ). Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44, 283–292.[CrossRef]
    [Google Scholar]
  13. Kuo, L., Grosfeld, H., Cristina, J., Hill, M. G. & Collins, P. L. ( 1996; ). Effect of mutations in the gene-start and gene-end sequence motifs on transcription of monocistronic and dicistronic minigenomes of respiratory syncytial virus. J Virol 70, 6892–6901.
    [Google Scholar]
  14. Kuo, L., Fearns, R. & Collins, P. L. ( 1997; ). Analysis of the gene start and gene end signals of human respiratory syncytial virus: quasi-templated initiation at position 1 of the encoded mRNA. J Virol 71, 4944–4953.
    [Google Scholar]
  15. Li, J., Ling, R., Randhawa, J. S., Shaw, K., Davis, P. J., Juhasz, K., Pringle, C. R., Easton, A. J. & Cavanagh, D. ( 1996; ). Sequence of the nucleocapsid protein gene of subgroup A and B avian pneumoviruses. Virus Res 41, 185–191.[CrossRef]
    [Google Scholar]
  16. Ling, R. & Pringle, C. R. ( 1988; ). Turkey rhinotracheitis virus: in vivo and in vitro polypeptide synthesis. J Gen Virol 69, 917–923.[CrossRef]
    [Google Scholar]
  17. Ling, R., Easton, A. J. & Pringle, C. R. ( 1992; ). Sequence analysis of the 22K, SH and G genes of the turkey rhinotracheitis virus and their intergenic regions reveal a gene order different from that of other pneumoviruses. J Gen Virol 73, 1709–1715.[CrossRef]
    [Google Scholar]
  18. Ling, R., Davis, P. J., Yu, Q., Wood, C. M., Pringle, C. R., Cavanagh, D. & Easton, A. J. ( 1995; ). Sequence and in vitro expression of the phosphoprotein gene of avian pneumovirus. Virus Res 36, 247–257.[CrossRef]
    [Google Scholar]
  19. Lwamba, H. C., Bennett, R. S., Lauer, D. C., Halvorson, D. A. & Njenga, M. K. ( 2002; ). Characterization of avian metapneumoviruses isolated in the USA. Anim Health Res Rev 3, 107–117.[CrossRef]
    [Google Scholar]
  20. Marriott, A. C., Wilson, S. D., Randhawa, J. S. & Easton, A. J. ( 1999; ). A single amino acid substitution in the phosphoprotein of respiratory syncytial virus confers thermosensitivity in a reconstituted RNA polymerase system. J Virol 73, 5162–5165.
    [Google Scholar]
  21. Marriott, A. C., Smith, J. M. & Easton, A. J. ( 2001; ). Fidelity of leader and trailer sequence usage by the respiratory syncytial virus and avian pneumovirus replication complexes. J Virol 75, 6265–6272.[CrossRef]
    [Google Scholar]
  22. Naylor, C. J., Brown, P. A., Edworthy, N., Ling, R., Jones, R. C., Savage, C. E. & Easton, A. J. ( 2004; ). Development of a reverse-genetics system for avian pneumovirus demonstrates that the small hydrophobic (SH) and attachment (G) genes are not essential for virus viability. J Gen Virol 85, 3219–3227.[CrossRef]
    [Google Scholar]
  23. Pringle, C. R. & Easton, A. J. ( 1997; ). Monopartite negative strand RNA genomes. Semin Virol 8, 49–57.[CrossRef]
    [Google Scholar]
  24. Randhawa, J. S., Wilson, S. D., Tolley, K. P., Cavanagh, D., Pringle, C. R. & Easton, A. J. ( 1996; ). Nucleotide sequence of the gene encoding the viral polymerase of avian pneumovirus. J Gen Virol 77, 3047–3051.[CrossRef]
    [Google Scholar]
  25. Randhawa, J. S., Marriott, A. C., Pringle, C. R. & Easton, A. J. ( 1997; ). Rescue of synthetic minireplicons establishes the absence of the NS1 and NS2 genes from avian pneumovirus. J Virol 71, 9849–9854.
    [Google Scholar]
  26. Shin, H. J., Njenga, M. K., McComb, B., Halvorson, D. A. & Nagaraja, K. V. ( 2000; ). Avian pneumovirus (APV) RNA from wild and sentinel birds in the United States has genetic homology with RNA from APV isolates from domestic turkeys. J Clin Microbiol 38, 4282–4284.
    [Google Scholar]
  27. Shin, H. J., Nagaraja, K. V., McComb, B., Halvorson, D. A., Jirjis, F. F., Shaw, D. P., Seal, B. S. & Njenga, M. K. ( 2002; ). Isolation of avian pneumovirus from mallard ducks that is genetically similar to viruses isolated from neighboring commercial turkeys. Virus Res 83, 207–212.[CrossRef]
    [Google Scholar]
  28. Yu, Q., Davis, P. J., Barrett, T., Bimms, M. M., Boursnell, M. E. G. & Cavanagh, D. ( 1991; ). Deduced amino acid sequence of the fusion glycoprotein of turkey rhinotracheitis virus has greater identity with that of human respiratory syncytial virus, a pneumovirus, than that of paramyxoviruses and morbilliviruses. J Gen Virol 72, 75–81.[CrossRef]
    [Google Scholar]
  29. Yu, Q., Davis, P. J., Brown, T. D. K. & Cavanagh, D. ( 1992a; ). Sequence and in vitro expression of the M2 gene of turkey rhinotracheitis virus. J Gen Virol 73, 1355–1363.[CrossRef]
    [Google Scholar]
  30. Yu, Q., Davis, P. J., Li, J. & Cavanagh, D. ( 1992b; ). Cloning and sequencing the matrix protein (M) gene of turkey rhinotracheitis virus reveal a gene order different from that of respiratory syncytial virus. Virology 186, 426–434.[CrossRef]
    [Google Scholar]
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