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1887

Journal of General Virology header logo Journal of General Virology

Volume 87, Issue 3

The glycosylation site in the envelope protein of (Sarafend) plays an important role in replication and maturation processes No Access

Abstract

The complete genome of West Nile (Sarafend) virus [WN(S)V] was sequenced. Phylogenetic trees utilizing the complete genomic sequence, capsid gene, envelope gene and NS5 gene/3′ untranslated region of WN(S)V classified WN(S)V as a lineage II virus. A full-length infectious clone of WN(S)V with a point mutation in the glycosylation site of the envelope protein (pWNS-S154A) was constructed. Both growth kinetics and the mode of maturation were affected by this mutation. The titre of the pWNS-S154A virus was lower than the wild-type virus. This defect was corrected by the expression of wild-type envelope protein in . The pWNS-S154A virus matured intracellularly instead of at the plasma membrane as shown for the parental WN(S)V.

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/content/journal/jgv/10.1099/vir.0.81320-0
2006-03-01
2025-02-06
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The glycosylation site in the envelope protein of West Nile virus (Sarafend) plays an important role in replication and maturation processes
J. Gen. Virol. 87, 613 (2006); https://doi.org/10.1099/vir.0.81320-0
/content/journal/jgv/10.1099/vir.0.81320-0
/content/journal/jgv/10.1099/vir.0.81320-0
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