1887

Abstract

An orbivirus designated (YUOV) was isolated from mosquitoes collected in the Yunnan province of China. Electron microscopy showed particles with typical orbivirus morphology. The YUOV genome was sequenced completely and compared with previously characterized orbivirus genomes. Significant identity scores were detected between proteins encoded by the segments (Seg-1 to Seg-10) of YUOV and those encoded by their homologues in insect-borne and tick-borne orbiviruses. Analysis of VP1 (Pol) and VP2 (T2, which correlates with the virus serogroup) indicated that YUOV is a new species of the genus that is unrelated to the other insect-borne orbiviruses. The replication of YUOV in mosquito cell lines was restricted to cells and the virus failed to replicate in mammalian cell lines. However, intraperitoneal injection of virus into naïve mice resulted in productive, non-lethal virus replication and viraemia. Infected mice developed serum neutralizing antibodies and were protected against a new infection challenge. Sequence analysis of clones from the segments encoding outer coat proteins (Seg-3 and Seg-6) of YUOV recovered from mouse blood did not show significant changes in the sequences. The availability of the complete genome sequence will facilitate the development of sequence-specific PCR assays for the study of YUOV epidemiology in the field.

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2005-12-01
2019-11-21
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References

  1. Attoui, H., Billoir, F., Cantaloube, J. F., Biagini, P., de Micco, P. & de Lamballerie, X. ( 2000a; ). Strategies for the sequence determination of viral dsRNA genomes. J Virol Methods 89, 147–158.[CrossRef]
    [Google Scholar]
  2. Attoui, H., Billoir, F., Biagini, P., de Micco, P. & de Lamballerie, X. ( 2000b; ). Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae. J Gen Virol 81, 1507–1515.
    [Google Scholar]
  3. Attoui, H., Stirling, J. M., Munderloh, U. G., Billoir, F., Brookes, S. M., Burroughs, J. N., de Micco, P., Mertens, P. P. C. & de Lamballerie, X. ( 2001; ). Complete sequence characterization of the genome of the St Croix River virus, a new orbivirus isolated from cells of Ixodes scapularis. J Gen Virol 82, 795–804.
    [Google Scholar]
  4. Attoui, H., Fang, Q., Mohd Jaafar, F., Cantaloube, J-F., Biagini, P., de Micco, P. & de Lamballerie, X. ( 2002; ). Common evolutionary origin of aquareoviruses and orthoreoviruses revealed by genome characterization of Golden shiner reovirus, Grass carp reovirus, Striped bass reovirus and golden ide reovirus (genus Aquareovirus, family Reoviridae). J Gen Virol 83, 1941–1951.
    [Google Scholar]
  5. Burroughs, J. N., O'Hara, R. S., Smale, C. J., Hamblin, C., Walton, A., Armstrong, R. & Mertens, P. P. C. ( 1994; ). Purification and properties of virus particles, infectious subviral particles, cores and VP7 crystals of African horsesickness virus serotype 9. J Gen Virol 75, 1849–1857.[CrossRef]
    [Google Scholar]
  6. Das, B. P., Lal, S. & Saxena, V. K. ( 2004; ). Outdoor resting preference of Culex tritaeniorhynchus, the vector of Japanese encephalitis in Warangal and Karim Nagar districts, Andhra Pradesh. J Vector Borne Dis 41, 32–36.
    [Google Scholar]
  7. Gouet, P., Diprose, J. M., Grimes, J. M., Malby, R., Burroughs, J. N., Zientara, S., Stuart, D. I. & Mertens, P. P. C. ( 1999; ). The highly ordered double-stranded RNA genome of bluetongue virus revealed by crystallography. Cell 97, 481–490.[CrossRef]
    [Google Scholar]
  8. Grimes, J. M., Burroughs, J. N., Gouet, P., Diprose, J. M., Malby, R., Ziéntara, S., Mertens, P. P. C. & Stuart, D. I. ( 1998; ). The atomic structure of the bluetongue virus core. Nature 395, 470–478.[CrossRef]
    [Google Scholar]
  9. Herring, B. L., Tsui, R., Peddada, L., Busch, M. & Delwart, E. L. ( 2005; ). Wide range of quasispecies diversity during primary hepatitis C virus infection. J Virol 79, 4340–4346.[CrossRef]
    [Google Scholar]
  10. Hooper, P. T., Lunt, R. A., Gould, A. R. & 10 other authors ( 1999; ). Epidemic of blindness in kangaroos – evidence of a viral aetiology. Aust Vet J 77, 529–536.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. Mertens, P. P. C. ( 1999; ). Orbiviruses and coltiviruses – general features. In Encyclopedia of Virology, 2nd edn, pp. 1043–1061. Edited by A. Granoff & R. G. Webster. London: Academic Press.
  13. Mertens, P. P. C., Arella, M., Attoui, H. & 41 other authors ( 2000; ). Family Reoviridae. In Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses, pp. 399–480. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. New York: Academic Press.
  14. Mertens, P. P. C., Attoui, H., Duncan, R. & Dermody, T. S. ( 2005; ). Family Reoviridae. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, pp. 447–454. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier/Academic Press.
  15. Moss, S. R. & Nuttall, P. A. ( 1994; ). Subcore and core-like particles of Broadhaven virus (BRDV), a tick-borne orbivirus, synthesized from baculovirus expressed VP2 and VP7, the major core proteins of BRDV. Virus Res 32, 401–407.[CrossRef]
    [Google Scholar]
  16. Moss, S. R., Jones, L. D. & Nuttall, P. A. ( 1992; ). Comparison of the major structural core proteins of tick-borne and Culicoides-borne orbiviruses. J Gen Virol 73, 2585–2590.[CrossRef]
    [Google Scholar]
  17. Parkes, H. & Gould, A. R. ( 1996; ). Characterisation of Wongorr virus, an Australian orbivirus. Virus Res 44, 111–122.[CrossRef]
    [Google Scholar]
  18. Pritchard, L. I., Gould, A. R., Wilson, W. C., Thompson, L., Mertens, P. P. C. & Wade-Evans, A. M. ( 1995; ). Complete nucleotide sequence of RNA segment 3 of bluetongue virus serotype 2 (Ona-A). Phylogenetic analyses reveal the probable origin and relationship with other orbiviruses. Virus Res 35, 247–261.[CrossRef]
    [Google Scholar]
  19. Pugachev, K. V., Guirakhoo, F., Ocran, S. W. & 8 other authors ( 2004; ). High fidelity of yellow fever virus RNA polymerase. J Virol 78, 1032–1038.[CrossRef]
    [Google Scholar]
  20. Roy, P. & Mertens, P. P. C. ( 1999; ). Orbiviruses and coltiviruses – molecular biology. In Encyclopedia of Virology, 2nd edn, pp. 1062–1074. Edited by A. Granoff & R. G. Webster. London: Academic Press.
  21. Smith, D. B., McAllister, J., Casino, C. & Simmonds, P. ( 1997; ). Virus ‘quasispecies’: making a mountain out of a molehill? J Gen Virol 78, 1511–1519.
    [Google Scholar]
  22. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
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Supplements

vol. , part 12, pp. 3409 – 3417

Sequences used in phylogenetic analysis of (YUOV)

Sequences used in phylogenetic analysis of the T2 protein of YUOV

Alignment of protein sequences from PCR clones of segment 3 (VP3) of YUOV from mouse blood

Alignment of protein sequences from PCR clones of segment 6 (VP5) of YUOV from mouse blood

Alignment of protein sequences from PCR clones of segment 7 (NS2) of YUOV from mouse blood

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