1887

Abstract

Genomic recombination between different genotypes of (HBV) resulting in hybrid strains has been increasingly documented. In this study, a novel recombinant of HBV genotypes G and C isolated from a Thai patient with hepatocellular carcinoma is reported. Based on phylogenetic analyses of the S, P and X genes and the entire genome, the HBV isolate clustered on a branch within genotype G, but clustered with genotype C on analysis of the C gene. Using the program and bootscanning analysis, the recombination breakpoints were located at nt 1860 and 2460 of the precore/core region. The hallmarks of the original genotype G, including a 36 bp insertion in the core region and dual stop codons in the precore region, were not identified in this isolate. These data should encourage further investigations on the epidemiological and virological characteristics of HBV genotype G involved in recombination with other genotypes.

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2005-11-01
2019-10-14
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References

  1. Bollyky, P. L., Rambaut, A., Harvey, P. H. & Holmes, E. C. ( 1996; ). Recombination between sequences of hepatitis B virus from different genotypes. J Mol Evol 42, 97–102.[CrossRef]
    [Google Scholar]
  2. Bowyer, S. M. & Sim, J. G. M. ( 2000; ). Relationships within and between genotypes of hepatitis B virus at points across the genome: footprints of recombination in certain isolates. J Gen Virol 81, 379–392.
    [Google Scholar]
  3. Cui, C., Shi, J., Hui, L., Xi, H., Zhuoma, Quni, Tsedan & Hu, G. ( 2002; ). The dominant hepatitis B virus genotype identified in Tibet is a C/D hybrid. J Gen Virol 83, 2773–2777.
    [Google Scholar]
  4. Fares, M. A. & Holmes, E. C. ( 2002; ). A revised evolutionary history of hepatitis B virus (HBV). J Mol Evol 54, 807–814.[CrossRef]
    [Google Scholar]
  5. Ganem, D. & Prince, A. M. ( 2004; ). Hepatitis B virus infection – natural history and clinical consequences. N Engl J Med 350, 1118–1129.[CrossRef]
    [Google Scholar]
  6. Hannoun, C., Norder, H. & Lindh, M. ( 2000; ). An aberrant genotype revealed in recombinant hepatitis B virus strains from Vietnam. J Gen Virol 81, 2267–2272.
    [Google Scholar]
  7. Hino, O., Tabata, S. & Hotta, Y. ( 1991; ). Evidence for increased in vitro recombination with insertion of human hepatitis B virus DNA. Proc Natl Acad Sci U S A 88, 9248–9252.[CrossRef]
    [Google Scholar]
  8. Kato, H., Orito, E., Gish, R. G. & 7 other authors ( 2002a; ). Hepatitis B e antigen in sera from individuals infected with hepatitis B virus of genotype G. Hepatology 35, 922–929.[CrossRef]
    [Google Scholar]
  9. Kato, H., Orito, E., Gish, R. G., Sugauchi, F., Suzuki, S., Ueda, R., Miyakawa, Y. & Mizokami, M. ( 2002b; ). Characteristics of hepatitis B virus isolates of genotype G and their phylogenetic differences from the other six genotypes (A through F). J Virol 76, 6131–6137.[CrossRef]
    [Google Scholar]
  10. Lole, K. S., Bollinger, R. C., Paranjape, R. S., Gadkari, D., Kulkarni, S. S., Novak, N. G., Ingersoll, R., Sheppard, H. W. & Ray, S. C. ( 1999; ). Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 73, 152–160.
    [Google Scholar]
  11. Luo, K., Liu, Z., He, H., Peng, J., Liang, W., Dai, W. & Hou, J. ( 2004; ). The putative recombination of hepatitis B virus genotype B with pre-C/C region of genotype C. Virus Genes 29, 31–41.[CrossRef]
    [Google Scholar]
  12. Morozov, V., Pisareva, M. & Groudinin, M. ( 2000; ). Homologous recombination between different genotypes of hepatitis B virus. Gene 260, 55–65.[CrossRef]
    [Google Scholar]
  13. Okamoto, H., Yotsumoto, S., Akahane, Y. & 7 other authors ( 1990; ). Hepatitis B viruses with precore region defects prevail in persistently infected hosts along with seroconversion to the antibody against e antigen. J Virol 64, 1298–1303.
    [Google Scholar]
  14. Osiowy, C. & Giles, E. ( 2003; ). Evaluation of the INNO-LiPA HBV genotyping assay for determination of hepatitis B virus genotype. J Clin Microbiol 41, 5473–5477.[CrossRef]
    [Google Scholar]
  15. Owiredu, W. K. B. A., Kramvis, A. & Kew, M. C. ( 2001; ). Hepatitis B virus DNA in serum of healthy black African adults positive for hepatitis B surface antibody alone: possible association with recombination between genotypes A and D. J Med Virol 64, 441–454.[CrossRef]
    [Google Scholar]
  16. Pineau, P., Marchio, A., Mattei, M.-G., Kim, W.-H., Youn, J.-K., Tiollais, P. & Dejean, A. ( 1998; ). Extensive analysis of duplicated-inverted hepatitis B virus integrations in human hepatocellular carcinoma. J Gen Virol 79, 591–600.
    [Google Scholar]
  17. Salminen, M. O., Carr, J. K., Burke, D. S. & McCutchan, F. E. ( 1995; ). Identification of breakpoints in intergenotypic recombinants of HIV type 1 by bootscanning. AIDS Res Hum Retroviruses 11, 1423–1425.[CrossRef]
    [Google Scholar]
  18. Schaefer, S. ( 2005; ). Hepatitis B virus: significance of genotypes. J Viral Hepat 12, 111–124.[CrossRef]
    [Google Scholar]
  19. Stuyver, L., De Gendt, S., Van Geyt, C., Zoulim, F., Fried, M., Schinazi, R. F. & Rossau, R. ( 2000; ). A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. J Gen Virol 81, 67–74.
    [Google Scholar]
  20. Sugauchi, F., Orito, E., Ichida, T. & 9 other authors ( 2002; ). Hepatitis B virus of genotype B with or without recombination with genotype C over the precore region plus the core gene. J Virol 76, 5985–5992.[CrossRef]
    [Google Scholar]
  21. Tangkijvanich, P., Mahachai, V., Komolmit, P., Fongsarun, J., Theamboonlers, A. & Poovorawan, Y. ( 2005; ). Hepatitis B virus genotypes and hepatocellular carcinoma in Thailand. World J Gastroenterol 11, 2238–2243.[CrossRef]
    [Google Scholar]
  22. Wang, Z., Liu, Z., Zeng, G., Wen, S., Qi, Y., Ma, S., Naoumov, N. V. & Hou, J. ( 2005; ). A new intertype recombinant between genotypes C and D of hepatitis B virus identified in China. J Gen Virol 86, 985–990.[CrossRef]
    [Google Scholar]
  23. Weber, B. ( 2005; ). Genetic variability of the S gene of hepatitis B virus: clinical and diagnostic impact. J Clin Virol 32, 102–112.[CrossRef]
    [Google Scholar]
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