1887

Abstract

Hepatitis C virus (HCV) binding to hepatocytes is thought to be mediated via interaction of the E2 glycoprotein with (co-)receptors including CD81 and scavenger receptor class B type I (SR-BI). Here, the expression of CD81 and SR-BI was analysed on peripheral blood mononuclear cell (PBMC) subsets, and the binding of genotype 1 soluble truncated E2 (sE2) proteins to these cells was investigated. All PBMC subsets expressed CD81, although at varying levels. In contrast, SR-BI was only detected on monocytes and dendritic cells (DCs). The genotype 1a H77c sE2 protein showed higher PBMC binding than other genotype 1a/b sE2s. H77c sE2 binding to different PBMC subsets largely paralleled their level of CD81 expression, and could be inhibited by blocking E2–CD81 interaction. However, those PBMC subsets reported to be infected by HCV (monocytes, DCs and B cells) also exhibited residual, CD81-independent binding, indicating roles for SR-BI/other receptor(s) in mediating haematopoietic cell infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81169-0
2005-09-01
2019-11-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/9/vir862507.html?itemId=/content/journal/jgv/10.1099/vir.0.81169-0&mimeType=html&fmt=ahah

References

  1. Agnello, V., Abel, G., Elfahal, M., Knight, G. B. & Zhang, Q. X. ( 1999; ). Hepatitis C virus and other flaviviridae viruses enter cells via low density lipoprotein receptor. Proc Natl Acad Sci U S A 96, 12766–12771.[CrossRef]
    [Google Scholar]
  2. Bartosch, B., Dubuisson, J. & Cosset, F. L. ( 2003a; ). Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes. J Exp Med 197, 633–642.[CrossRef]
    [Google Scholar]
  3. Bartosch, B., Vitelli, A., Granier, C. & 7 other authors ( 2003b; ). Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor. J Biol Chem 278, 41624–41630.[CrossRef]
    [Google Scholar]
  4. Basu, A., Beyene, A., Meyer, K. & Ray, R. ( 2004; ). The hypervariable region 1 of the E2 glycoprotein of hepatitis C virus binds to glycosaminoglycans, but this binding does not lead to infection in a pseudotype system. J Virol 78, 4478–4486.[CrossRef]
    [Google Scholar]
  5. Bocharov, A. V., Baranova, I. N., Vishnyakova, T. G., Remaley, A. T., Csako, G., Thomas, F., Patterson, A. P. & Eggerman, T. L. ( 2004; ). Targeting of scavenger receptor class B type I by synthetic amphipathic α-helical-containing peptides blocks lipopolysaccharide (LPS) uptake and LPS-induced pro-inflammatory cytokine responses in THP-1 monocyte cells. J Biol Chem 279, 36072–36082.[CrossRef]
    [Google Scholar]
  6. Buechler, C., Ritter, M., Quoc, C. D., Agildere, A. & Schmitz, G. ( 1999; ). Lipopolysaccharide inhibits the expression of the scavenger receptor Cla-1 in human monocytes and macrophages. Biochem Biophys Res Commun 262, 251–254.[CrossRef]
    [Google Scholar]
  7. Cormier, E. G., Tsamis, F., Kajumo, F., Durso, R. J., Gardner, J. P. & Dragic, T. ( 2004; ). CD81 is an entry coreceptor for hepatitis C virus. Proc Natl Acad Sci U S A 101, 7270–7274.[CrossRef]
    [Google Scholar]
  8. Crotta, S., Stilla, A., Wack, A. & 9 other authors ( 2002; ). Inhibition of natural killer cells through engagement of CD81 by the major hepatitis C virus envelope protein. J Exp Med 195, 35–41.[CrossRef]
    [Google Scholar]
  9. Engering, A., Van Vliet, S. J., Geijtenbeek, T. B. & Van Kooyk, Y. ( 2002; ). Subset of DC-SIGN+ dendritic cells in human blood transmits HIV-1 to T lymphocytes. Blood 100, 1780–1786.[CrossRef]
    [Google Scholar]
  10. Ferri, C. & Zignego, A. L. ( 2000; ). Relation between infection and autoimmunity in mixed cryoglobulinemia. Curr Opin Rheumatol 12, 53–60.[CrossRef]
    [Google Scholar]
  11. Flint, M., Maidens, C., Loomis-Price, L. D., Shotton, C., Dubuisson, J., Monk, P., Higginbottom, A., Levy, S. & McKeating, J. A. ( 1999; ). Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81. J Virol 73, 6235–6244.
    [Google Scholar]
  12. Gardner, J. P., Durso, R. J., Arrigale, R. R., Donovan, G. P., Maddon, P. J., Dragic, T. & Olson, W. C. ( 2003; ). L-SIGN (CD 209L) is a liver-specific capture receptor for hepatitis C virus. Proc Natl Acad Sci U S A 100, 4498–4503.[CrossRef]
    [Google Scholar]
  13. Hsu, M., Zhang, J., Flint, M., Logvinoff, C., Cheng-Mayer, C., Rice, C. M. & McKeating, J. A. ( 2003; ). Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles. Proc Natl Acad Sci U S A 100, 7271–7276.[CrossRef]
    [Google Scholar]
  14. Imachi, H., Murao, K., Hiramine, C. & 8 other authors ( 2000; ). Human scavenger receptor B1 is involved in recognition of apoptotic thymocytes by thymic nurse cells. Lab Invest 80, 263–270.[CrossRef]
    [Google Scholar]
  15. Kjellen, L. & Lindahl, U. ( 1991; ). Proteoglycans: structures and interactions. Annu Rev Biochem 60, 443–475.[CrossRef]
    [Google Scholar]
  16. Krieger, M. ( 2001; ). Scavenger receptor class B type I is a multiligand HDL receptor that influences diverse physiologic systems. J Clin Invest 108, 793–797.[CrossRef]
    [Google Scholar]
  17. Lavillette, D., Tarr, A. W., Voisset, C. & 7 other authors ( 2005; ). Characterization of host-range and cell entry properties of the major genotypes and subtypes of hepatitis C virus. Hepatology 41, 265–274.[CrossRef]
    [Google Scholar]
  18. Levy, S., Todd, S. C. & Maecker, H. T. ( 1998; ). CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system. Annu Rev Immunol 16, 89–109.[CrossRef]
    [Google Scholar]
  19. Lozach, P. Y., Lortat-Jacob, H., de Lacroix de Lavalette, A. & 9 other authors ( 2003; ). DC-SIGN and L-SIGN are high affinity binding receptors for hepatitis C virus glycoprotein E2. J Biol Chem 278, 20358–20366.[CrossRef]
    [Google Scholar]
  20. Lozach, P. Y., Amara, A., Bartosch, B., Virelizier, J. L., Arenzana-Seisdedos, F., Cosset, F. L. & Altmeyer, R. ( 2004; ). C-type lectins L-SIGN and DC-SIGN capture and transmit infectious hepatitis C virus pseudotype particles. J Biol Chem 279, 32035–32045.[CrossRef]
    [Google Scholar]
  21. McKeating, J. A., Zhang, L. Q., Logvinoff, C. & 8 other authors ( 2004; ). Diverse hepatitis C virus glycoproteins mediate viral infection in a CD81-dependent manner. J Virol 78, 8496–8505.[CrossRef]
    [Google Scholar]
  22. Patel, A. H., Wood, J., Penin, F., Dubuisson, J. & McKeating, J. A. ( 2000; ). Construction and characterization of chimeric hepatitis C virus E2 glycoproteins: analysis of regions critical for glycoprotein aggregation and CD81 binding. J Gen Virol 81, 2873–2883.
    [Google Scholar]
  23. Pearson, A. M. ( 1996; ). Scavenger receptors in innate immunity. Curr Opin Immunol 8, 20–28.[CrossRef]
    [Google Scholar]
  24. Pileri, P., Uematsu, Y., Campagnoli, S. & 8 other authors ( 1998; ). Binding of hepatitis C virus to CD81. Science 282, 938–941.[CrossRef]
    [Google Scholar]
  25. Pohlmann, S., Zhang, J., Baribaud, F. & 7 other authors ( 2003; ). Hepatitis C virus glycoproteins interact with DC-SIGN and DC- SIGNR. J Virol 77, 4070–4080.[CrossRef]
    [Google Scholar]
  26. Roccasecca, R., Ansuini, H., Vitelli, A. & 11 other authors ( 2003; ). Binding of the hepatitis C virus E2 glycoprotein to CD81 is strain specific and is modulated by a complex interplay between hypervariable regions 1 and 2. J Virol 77, 1856–1867.[CrossRef]
    [Google Scholar]
  27. Scarselli, E., Ansuini, H., Cerino, R. & 7 other authors ( 2002; ). The human scavenger receptor class B type I is a novel candidate receptor for the hepatitis C virus. EMBO J 21, 5017–5025.[CrossRef]
    [Google Scholar]
  28. Silver, D. L. & Tall, A. R. ( 2001; ). The cellular biology of scavenger receptor class B type I. Curr Opin Lipidol 12, 497–504.[CrossRef]
    [Google Scholar]
  29. Takikawa, S., Ishii, K., Aizaki, H., Suzuki, T., Asakura, H., Matsuura, Y. & Miyamura, T. ( 2000; ). Cell fusion activity of hepatitis C virus envelope proteins. J Virol 74, 5066–5074.[CrossRef]
    [Google Scholar]
  30. Triyatni, M., Vergalla, J., Davis, A. R., Hadlock, K. G., Foung, S. K. & Liang, T. J. ( 2002; ). Structural features of envelope proteins on hepatitis C virus-like particles as determined by anti-envelope monoclonal antibodies and CD81 binding. Virology 298, 124–132.[CrossRef]
    [Google Scholar]
  31. Tseng, C. T. & Klimpel, G. R. ( 2002; ). Binding of the hepatitis C virus envelope protein E2 to CD81 inhibits natural killer cell functions. J Exp Med 195, 43–49.[CrossRef]
    [Google Scholar]
  32. Turville, S. G., Arthos, J., Donald, K. M., Lynch, G., Naif, H., Clark, G., Hart, D. & Cunningham, A. L. ( 2001; ). HIV gp120 receptors on human dendritic cells. Blood 98, 2482–2488.[CrossRef]
    [Google Scholar]
  33. Wack, A., Soldaini, E., Tseng, C. T. K., Nuti, S., Kimpel, G. R. & Abrignani, S. ( 2001; ). Binding of the hepatitis C virus envelope protein E2 to CB81 provides a co-stimulatory signal for human T cells. Eur J Immunol 31, 166–175.[CrossRef]
    [Google Scholar]
  34. Wunschmann, S., Medh, J. D., Klinzmann, D., Schmidt, W. N. & Stapleton, J. T. ( 2000; ). Characterization of hepatitis C virus (HCV) and HCV E2 interactions with CD81 and the low-density lipoprotein receptor. J Virol 74, 10055–10062.[CrossRef]
    [Google Scholar]
  35. Yagnik, A. T., Lahm, A., Meola, A., Roccasecca, R. M., Ercole, B. B., Nicosia, A. & Tramontano, A. ( 2000; ). A model for the hepatitis C virus envelope glycoprotein E2. Proteins 40, 355–366.[CrossRef]
    [Google Scholar]
  36. Zhang, J., Randall, G., Higginbottom, A., Monk, P., Rice, C. M. & McKeating, J. A. ( 2004; ). CD81 is required for hepatitis C virus glycoprotein-mediated viral infection. J Virol 78, 1448–1455.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.81169-0
Loading
/content/journal/jgv/10.1099/vir.0.81169-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 2507 - 2512

Expression of CD81 and SR-BI on monocyte-derived DCs (MDDCs), and binding of H77c E2660 to these cells.

Alignment of the sequences of truncated E2660/661 proteins from H77c, UKN1A14, UKN1B12 and BK HCV strains.

Western blot analysis of H77c, UKN1A14, UKN1B12 and BK sE2 protein preparations normalized on the basis of monomeric E2 content.

Summary of the PBMC subsets studied.

[Single PDF file](198 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error