%0 Journal Article %A Hazari, Sidhartha %A Patil, Asha %A Joshi, Virendra %A Sullivan, Deborah E. %A Fermin, Cesar D. %A Garry, Robert F. %A Elliott, Richard M. %A Dash, Srikanta %T Alpha interferon inhibits translation mediated by the internal ribosome entry site of six different hepatitis C virus genotypes %D 2005 %J Journal of General Virology, %V 86 %N 11 %P 3047-3053 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.81132-0 %I Microbiology Society, %X THIS ARTICLE HAS BEEN RETRACTEDCertain genotypes of hepatitis C virus (HCV) respond less often than others to treatment with interferon (IFN). The mechanisms for this differential response are not known. In this report antiviral effects of IFN-α2b on translation were examined in a hepatic cell line using chimeric clones of internal ribosome entry site (IRES) sequences from six different HCV genotypes and the green fluorescence protein (GFP) gene. As a control, IFN action at the level of the IRES was examined in the presence of different cytokines. It was determined that IFN-α2b specifically inhibited the translation of GFP mediated by IRES sequences from six major HCV genotypes in a concentration-dependent manner. Other cytokines including tumour necrosis factor alpha, transforming growth factor beta 1, interleukin 1 and interleukin 6 have no inhibitory effect. The inhibition of translation in these experiments was not due to extensive intracellular degradation of IRES-GFP mRNA. These results suggest that the antiviral action of IFN-α2b blocks IRES-mediated translation and this effect is the same among HCVs of other genotypes. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81132-0