1887

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Volume 87, Issue 4

Purification and characterization of infectious myonecrosis virus of penaeid shrimp Free

Abstract

The causative agent of myonecrosis affecting cultured in Brazil was demonstrated to be a virus after purification of the agent from infected shrimp tissues. Purified viral particles were injected into specific pathogen-free , resulting in a disease that displayed the same characteristics as those found in the original shrimp used for purification. The virus was named infectious myonecrosis virus (IMNV). The viral particles were icosahedral in shape and 40 nm in diameter, with a buoyant density of 1·366 g ml in caesium chloride. The genome consisted of a single, double-stranded (dsRNA) molecule of 7560 bp. Sequencing of the viral genome revealed two non-overlapping open reading frames (ORFs). The 5′ ORF (ORF 1, nt 136–4953) encoded a putative RNA-binding protein and a capsid protein. The coding region of the RNA-binding protein was located in the first half of ORF 1 and contained a dsRNA-binding motif in the first 60 aa. The second half of ORF 1 encoded a capsid protein, as determined by amino acid sequencing, with a molecular mass of 106 kDa. The 3′ ORF (ORF 2, nt 5241–7451) encoded a putative RNA-dependent RNA polymerase (RdRp) with motifs characteristic of totiviruses. Phylogenetic analysis based on the RdRp clustered IMNV with , a member of the family . Based on these findings, IMNV may be a unique member of the or may represent a new dsRNA virus family that infects invertebrate hosts.

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2006-04-01
2024-04-25
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References

  1. Altschul S. F., Erickson B. W. 1985; Significance of nucleotide sequence alignments: a method for random sequence permutation that preserves dinucleotide and codon usage. Mol Biol Evol 2:526–538
     [Google Scholar]
  2. Attoui H., Charrel R. N., Billoir F., Cantaloube J.-F., de Micco P., de Lamballerie X. 1998; Comparative sequence analysis of American, European and Asian isolates of viruses in the genus Coltivirus . J Gen Virol 79:2481–2489
     [Google Scholar]
  3. Bell T. A., Lightner D. V. 1988 A Handbook of Normal Penaeid Shrimp Histology Baton Rouge, LA: World Aquaculture Society;
     [Google Scholar]
  4. Bessarab I. N., Liu H.-W., Ip C.-F., Tai J.-H. 2000; The complete cDNA sequence of a type II Trichomonas vaginalis virus. Virology 267:350–359 [CrossRef]
     [Google Scholar]
  5. Bonami J.-R., Hasson K. W., Mari J., Poulos B. T., Lightner D. V. 1997; Taura syndrome of marine penaeid shrimp: characterization of the viral agent. J Gen Virol 78:313–319
     [Google Scholar]
  6. Bruenn J. A. 1993; A closely related group of RNA-dependent RNA polymerases from double-stranded RNA viruses. Nucleic Acids Res 21:5667–5669 [CrossRef]
     [Google Scholar]
  7. Bruenn J. 2002; The double-stranded RNA viruses of Ustilago maydis and their killer toxins. In dsRNA Genetic Elements: Concepts and Applications in Agriculture . Forestry, and Medicine pp  109–124 Edited by Tavantzis S. M. Boca Raton, FL: CRC Press;
     [Google Scholar]
  8. Chang K.-Y., Ramos A. 2005; The double-stranded RNA-binding motif, a versatile macromolecular docking platform. FEBS J 272:2109–2117 [CrossRef]
     [Google Scholar]
  9. Dinman J. D., Icho T., Wickner R. B. 1991; A −1 ribosomal frameshift in a double-stranded RNA virus of yeast forms a gag–pol fusion protein. Proc Natl Acad Sci U S A 88:174–178 [CrossRef]
     [Google Scholar]
  10. Edgerton B., Owens L., Glasson B., de Beer S. 1994; Description of a small dsRNA virus from the freshwater crayfish Cherax quadricarinatus . Dis Aquat Organ 18:63–69 [CrossRef]
     [Google Scholar]
  11. Farabaugh P. J. 1996; Programmed translational frameshifting. Annu Rev Genet 30:507–528 [CrossRef]
     [Google Scholar]
  12. Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. (editors) 2005; Totiviridae . In Virus Taxonomy: Classification and Nomenclature of Viruses. Eighth Report of the International Committee on the Taxonomy of Viruses pp  571–580 San Francisco: Elsevier;
     [Google Scholar]
  13. Garlapati S., Wang C. C. 2005; Structural elements in the 5′ untranslated region of giardiavirus transcript essential for internal ribosome entry site-mediated translational initiation. Eukaryot Cell 4:742–754 [CrossRef]
     [Google Scholar]
  14. Holthius L. B. 1980; Shrimps and prawns of the world: an annotated catalogue of species of interest to fisheries. In FAO Species Catalogue: FAO Fisheries Synopsis 125:1 Rome: Food and Agricultural Organization of the United Nations;
     [Google Scholar]
  15. Jablonski S. A., Luo M., Morrow C. D. 1991; Enzymatic activity of poliovirus RNA polymerase mutants with single amino acid changes in the conserved YGDD amino acid motif. J Virol 65:4565–4572
     [Google Scholar]
  16. Johnson K. N., Christian P. D. 1998; The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family. J Gen Virol 79:191–203
     [Google Scholar]
  17. Kim S. N., Choi J. H., Park M. W., Jeong S. J., Han K. S., Kim H. J. 2005; Identification of the +1 ribosomal frameshifting site of LRV1-4 by mutational analysis. Arch Pharm Res 28:956–962 [CrossRef]
     [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685 [CrossRef]
     [Google Scholar]
  19. Lightner D. V., Pantoja C. R., Poulos B. T., Tang K. F. J., Redman R. M., Andreas T., Bonami J. R. 2004a; Infectious myonecrosis (IMN): a new virus disease of Litopenaeus vannamei . In Aquaculture 2004 Book of Abstracts p– 353 Baton Rouge, LA: World Aquaculture Society;
     [Google Scholar]
  20. Lightner D. V., Pantoja C. R., Poulos B. T., Tang K. F. J., Redman R. M., Pasos de Andrade T., Bonami J. R. 2004b; Infectious myonecrosis: new disease in Pacific white shrimp. Glob Aquac Advocate 7:85
     [Google Scholar]
  21. Lotz J. M. 1997; Viruses, biosecurity and specific pathogen-free stocks in shrimp aquaculture. World J Microbiol Biotechnol 13:405–413 [CrossRef]
     [Google Scholar]
  22. Marchler-Bauer A., Bryant S. H. 2004; CD-Search: protein domain annotations on the fly. Nucleic Acids Res 32:W327–W331 [CrossRef]
     [Google Scholar]
  23. Matsudaira P. 1987; Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262:10035–10038
     [Google Scholar]
  24. Mertens P. 2004; The dsRNA viruses. Virus Res 101:3–13 [CrossRef]
     [Google Scholar]
  25. Neoh S. H., Gordon C., Potter A., Zola H. 1986; The purification of mouse monoclonal antibodies from ascitic fluid. J Immunol Methods 91:231–235 [CrossRef]
     [Google Scholar]
  26. Nunes A. J. P., Cunha-Martins P., Vasconselos-Gesteira T. C. 2004; Carcinicultura ameaçada. Rev Panoram Aquic 83:37–51 (in Portuguese
    [Google Scholar]
  27. Rice P., Longden I., Bleasby A. 2000; emboss: the European molecular biology open software suite. Trends Genet 16:276–277 [CrossRef]
     [Google Scholar]
  28. Rivers T. M. 1937; Viruses and Koch's postulate. J Bacteriol 33:1–12
     [Google Scholar]
  29. Robalino J., Browdy C. L., Prior S., Metz A., Parnell P., Gross P., Warr G. 2004; Induction of antiviral immunity by double-stranded RNA in a marine invertebrate. J Virol 78:10442–10448 [CrossRef]
     [Google Scholar]
  30. Scheffter S. M., Ro Y. T., Chung I. K., Patterson J. L. 1995; The complete sequence of Leishmania RNA virus LRV2-1, a virus of an Old World parasite strain. Virology 212:84–90 [CrossRef]
     [Google Scholar]
  31. Schmidt H. A., Strimmer K., Vingron M., von Haeseler A. 2002; tree-puzzle: maximum likelihood phylogenetic analysis using quartets and parallel computing. Bioinformatics 18:502–504 [CrossRef]
     [Google Scholar]
  32. St Johnston D., Brown N. H., Gall J. G., Jantsch M. 1992; A conserved double-stranded RNA-binding domain. Proc Natl Acad Sci U S A 89:10979–10983 [CrossRef]
     [Google Scholar]
  33. Strimmer K., von Haeseler A. 1996; Quartet puzzling: a quartet maximum-likelihood method for reconstructing tree topologies. Mol Biol Evol 13:964–969 [CrossRef]
     [Google Scholar]
  34. Stuart K. D., Weeks R., Guilbride L., Myler P. J. 1992; Molecular organization of Leishmania RNA virus 1. Proc Natl Acad Sci U S A 92:8596–8600
     [Google Scholar]
  35. Tang K. F. J., Pantoja C. R., Poulos B. T., Redman R. M., Lightner D. V. 2005; In situ hybridization demonstrates that Litopenaeus vannamei , L. stylirostris and Penaeus monodon are susceptible to experimental infection with infectious myonecrosis virus (IMNV). Dis Aquat Organ 63:261–265 [CrossRef]
     [Google Scholar]
  36. Tuomivirta T. T., Hantula J. 2003; Two unrelated double-stranded RNA molecule patterns in Gremmeniella abietina type A code for putative viruses of the families Totiviridae and Partitiviridae . Arch Virol 148:2293–2305 [CrossRef]
     [Google Scholar]
  37. van der Wilk F., Dullemans A. M., Verbeck M., Van den Heuvel J. F. J. M. 1997; Nucleotide sequence and genomic organization of Acyrthosiphon pisum virus. Virology 238:353–362 [CrossRef]
     [Google Scholar]
  38. Wang A. L., Wang C. C. 1986; Discovery of a specific double-stranded RNA virus in Giardia lamblia . Mol Biochem Parasitol 21:269–276 [CrossRef]
     [Google Scholar]
  39. Wang A. L., Yang H.-M., Shen K. A., Wang C. C. 1993; Giardiavirus double-stranded RNA genome encodes a capsid polypeptide and a gag–pol-like fusion protein by a translational frameshift. Proc Natl Acad Sci U S A 90:8595–8599 [CrossRef]
     [Google Scholar]
  40. Weast R. C., Astle M. J. 1980; CRC Handbook of Chemistry and Physics . , 61st edn. pp D233–D234 Boca Raton, FL: CRC Press;
  41. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem 244:4406–4412
     [Google Scholar]
  42. White B. L., Schofield P. J., Poulos B. T., Lightner D. V. 2002; A laboratory challenge method for estimating Taura syndrome virus resistance in selected lines of Pacific white shrimp Litopenaeus vannamei . J World Aquac Soc 33:341–348 [CrossRef]
     [Google Scholar]
  43. Williamson C., von Wechmar M. B. 1992; Two novel viruses associated with severe disease symptoms of the green stinkbug Nezara viridula . J Gen Virol 73:2467–2471 [CrossRef]
     [Google Scholar]
  44. Wilson C. M. 1983; Staining of proteins on gels: comparison of dyes and procedures. Methods Enzymol 91:236–247
     [Google Scholar]
  45. Wyban J. A., Swingle J. S., Sweeney J. N., Pruder G. D. 1992; Development and commercial performance of high health shrimp using specific pathogen free (SPF) broodstock Penaeus vannamei . In Proceedings of the Special Session on Shrimp Farming pp  254–259 Edited by Wyban J. Baton Rouge, LA: World Aquaculture Society;
     [Google Scholar]
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Purification and characterization of infectious myonecrosis virus of penaeid shrimp
J. Gen. Virol. 87, 987 (2006); https://doi.org/10.1099/vir.0.81127-0
/content/journal/jgv/10.1099/vir.0.81127-0
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