1887

Abstract

(PLRV) encodes two capsid proteins, major protein (CP) and minor protein (P5), an extended version of the CP produced by occasional translational ‘readthrough’ of the CP gene. Immunogold electron microscopy showed that PLRV CP is located in the cytoplasm and also localized in the nucleus, preferentially targeting the nucleolus. The nucleolar localization of PLRV CP was also confirmed when it was expressed as a fusion with green fluorescent protein (GFP) via an vector. Mutational analysis identified a particular sequence within PLRV CP involved in nucleolar targeting [the nucleolar localization signal (NoLS)]. Minor protein P5 also contains the same NoLS, and was targeted to the nucleolus when it was expressed as a fusion with GFP from . However, P5–GFP lost its nucleolar localization in the presence of replicating PLRV.

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2005-10-01
2020-11-24
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References

  1. Bahner I., Lamb J., Mayo M. A., Hay R. T. 1990; Expression of the genome of potato leafroll virus: readthrough of the coat protein termination codon. J Gen Virol 71:2251–2256 [CrossRef]
    [Google Scholar]
  2. Barker H., McGeachy K. D., Ryabov E. V., Commandeur U., Mayo M. A., Taliansky M. 2001; Evidence for RNA-mediated defence effects on the accumulation of Potato leafroll virus . J Gen Virol 82:3099–3106
    [Google Scholar]
  3. Carmo-Fonseca M., Mendes-Soares L., Campos I. 2000; To be or not to be in the nucleolus. Nat Cell Biol 2:E107–112 [CrossRef]
    [Google Scholar]
  4. Cockell M. M., Gasser S. M. 1999; The nucleolus: nucleolar space for RENT. Curr Biol 9:R575–576 [CrossRef]
    [Google Scholar]
  5. Dolja V. V., Koonin E. V. 1991; Phylogeny of capsid proteins of small icosahedral RNA plant viruses. J Gen Virol 72:1481–1486 [CrossRef]
    [Google Scholar]
  6. Esau K., Hoefert L. L. 1972; Development of infection with beet western yellows virus in sugarbeet. Virology 48:724–738 [CrossRef]
    [Google Scholar]
  7. Franco-Lara L., McGeachy K. D., Commandeur U., Martin R. R., Mayo M. A., Barker H. 1999; Transformation of tobacco and potato with cDNA encoding the full-length genome of potato leafroll virus: evidence for a novel virus distribution and host effects on virus multiplication. J Gen Virol 80:2813–2822
    [Google Scholar]
  8. Garcia-Bustos J., Heitman J., Hall M. 1991; Nuclear protein localization. Biochim Biophys Acta 1071:83–101 [CrossRef]
    [Google Scholar]
  9. Gill C. C., Chong J. 1975; Development of the infection in oat leaves inoculated with barley yellow dwarf virus. Virology 66:440–453 [CrossRef]
    [Google Scholar]
  10. Hanover J. A. 1992; The nuclear pore: at the crossroads. FASEB J 6:2288–2295
    [Google Scholar]
  11. Hellens R. P., Edwards E. A., Leyland N. R., Bean S., Mullineaux P. M. 2000; pGreen: a versatile and flexible binary Ti vector for Agrobacterium -mediated plant transformation. Plant Mol Biol 42:819–832 [CrossRef]
    [Google Scholar]
  12. Higuchi R., Krummel B., Saaki R. K. 1988; A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res 16:7351–7367 [CrossRef]
    [Google Scholar]
  13. Hiscox J. A. 2002; The nucleolus – a gateway to viral infection?. Arch Virol 147:1077–1089 [CrossRef]
    [Google Scholar]
  14. Kim S. H., Ryabov E. V., Brown J. W. S., Taliansky M. 2004; Involvement of the nucleolus in plant virus systemic infection. Biochem Soc Trans 32:557–560 [CrossRef]
    [Google Scholar]
  15. Lamb J. W., Duncan G. H., Reavy B., Gildow F. E., Mayo M. A., Hay R. T. 1996; Assembly of virus-like particles in insect cells infected with a baculovirus containing a modified coat protein gene of potato leafroll luteovirus. J Gen Virol 77:1349–1358 [CrossRef]
    [Google Scholar]
  16. Lamond A. I., Earnshaw W. C. 1998; Structure and function in the nucleus. Science 280:547–553 [CrossRef]
    [Google Scholar]
  17. Mayo M. A., Ziegler-Graff V. 1996; Molecular biology of luteoviruses. Adv Virus Res 46:416–468
    [Google Scholar]
  18. Mukherjee K., Verma Y., Chakrabarti S. K., Singh M. N., Khurana S. M. P. 2003; Cloning and sequencing of coat protein gene of an Indian potato leaf roll virus (PLRV) isolate and its similarity with other members of luteoviridae. Virus Genes 26:247–253 [CrossRef]
    [Google Scholar]
  19. Nass P. H., Jakstys B. P., D'Arcy C. J. 1995; In situ localization of barley yellow dwarf virus coat protein in oats. Phytopathology 85:556–560 [CrossRef]
    [Google Scholar]
  20. Nass P. H., Domier L. L., Jakstys B. P., D'Arcy C. J. 1998; In situ localization of barley yellow dwarf virus-PAV 17-kDa protein and nucleic acids in oats. Phytopathology 88:1031–1039 [CrossRef]
    [Google Scholar]
  21. Nurkiyanova K. N., Ryabov E., Duncan G. H., Canto T., Gray S. M., Commandeur U., Mayo M. A., Taliansky M. E. 2000; Tagging Potato leafroll virus with the jellyfish green fluorescent protein gene. J Gen Virol 81:617–626
    [Google Scholar]
  22. Olson M. O. J., Dundr M., Szebeni A. 2000; The nucleolus: an old factory with unexpected capabilities. Trends Cell Biol 10:189–196 [CrossRef]
    [Google Scholar]
  23. Pederson T. 1998; The plurifunctional nucleolus. Nucleic Acids Res 26:3871–3876 [CrossRef]
    [Google Scholar]
  24. Pfeffer S., Dunoyer P., Heim F., Richards K. E., Jonard G., Ziegler-Graff V. 2002; P0 of beet western yellows virus is a suppressor of posttranscriptional gene silencing. J Virol 76:6815–6824 [CrossRef]
    [Google Scholar]
  25. Ryabov E. V., Oparka K. J., Santa Cruz S., Robinson D. J., Taliansky M. E. 1998; Intracellular location of two groundnut rosette umbravirus proteins delivered by PVX and TMV vectors. Virology 242:303–313 [CrossRef]
    [Google Scholar]
  26. Ryabov E. V., Fraser G., Mayo M. A., Barker H., Taliansky M. 2001; Umbravirus gene expression helps Potato leafroll virus to invade mesophyll tissues and to be transmitted mechanically between plants. Virology 286:363–372 [CrossRef]
    [Google Scholar]
  27. Ryabov E. V., Kim S. H., Taliansky M. 2004; Identification of nuclear localisation signal and nuclear export signal of the umbraviral long-distance RNA movement protein. J Gen Virol 85:1329–1333 [CrossRef]
    [Google Scholar]
  28. Savenkov E. I., Valkonen J. P. T. 2001; Potyviral helper-component proteinase expressed in transgenic plants enhances titers of Potato leafroll virus but does not elevate its phloem limitation. Virology 283:285–293 [CrossRef]
    [Google Scholar]
  29. Shepardson S., Esau K., McCrum R. 1980; Ultrastructure of potato leaf phloem infected with potato leaf roll virus. Virology 105:379–392 [CrossRef]
    [Google Scholar]
  30. Tacke E., Schmitz S., Prüfer D., Rohde W. 1993; Mutational analysis of the nucleic acid-binding 17 kDa phosphoprotein of potato leafroll luteovirus identifies an amphipathic α -helix as the domain for protein/protein interactions. Virology 197:274–282 [CrossRef]
    [Google Scholar]
  31. Taliansky M. E., Robinson D. J. 2003; Molecular biology of umbraviruses: phantom warriors. J Gen Virol 84:1951–1960 [CrossRef]
    [Google Scholar]
  32. Taliansky M., Mayo M. A., Barker H. 2003; Potato leafroll virus : a classic pathogen shows some new tricks. Mol Plant Pathol 4:81–89 [CrossRef]
    [Google Scholar]
  33. Taliansky M., Kim S. H., Mayo M. A., Kalinina N. O., Fraser G., McGeachy K. D., Barker H. 2004; Escape of a plant virus from amplicon-mediated RNA silencing is associated with biotic or abiotic stresses. Plant J 39:194–205 [CrossRef]
    [Google Scholar]
  34. Voinnet O., Pinto Y. M., Baulcombe D. C. 1999; Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses. Proc Natl Acad Sci U S A 96:14147–14152 [CrossRef]
    [Google Scholar]
  35. Waterhouse P. M., Smith N. A., Wang M. B. 1999; Virus resistance and gene silencing: killing the messenger. Trends Plant Sci 4:452–457 [CrossRef]
    [Google Scholar]
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