1887

Abstract

In order to evaluate the genetic variability of (PPV), the complete capsid protein sequences (VP1/VP2) from seven recent field isolates from Germany, one isolate from the UK and one German vaccine strain were sequenced and analysed, along with two American (NADL-2 and Kresse), three Asian and 22 Brazilian partial PPV sequences retrieved from GenBank. The analysis revealed a high degree of diversity: 1·2–2·6 % at the nucleotide level and 1·2–6·8 % at the amino acid level. Phylogenetic analysis defined two German clusters: one formed by four German isolates and the English, Asian and American sequences; and the second, distinct cluster formed by the other three of the seven German isolates examined. The latter cluster was still observed when the 22 partial sequences (853 nt of the 3′ terminus of the VP2 gene) from the Brazilian isolates were included in the analyses, indicating that the VP2 sequence determines the phylogeny.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81086-0
2006-02-01
2019-11-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/2/295.html?itemId=/content/journal/jgv/10.1099/vir.0.81086-0&mimeType=html&fmt=ahah

References

  1. Bergeron, J., Menezes, J. & Tijssen, P. ( 1993; ). Genomic organization and mapping of transcription and translation products of the NADL-2 strain of porcine parvovirus. Virology 197, 86–98.[CrossRef]
    [Google Scholar]
  2. Bergeron, J., Hébert, B. & Tijssen, P. ( 1996; ). Genome organization of the Kresse strain of porcine parvovirus: identification of the allotropic determinant and comparison with those of NADL-2 and field isolates. J Virol 70, 2508–2515.
    [Google Scholar]
  3. Chang, S.-F., Sgro, J. Y. & Parrish, C. R. ( 1992; ). Multiple amino acids in the capsid structure of canine parvovirus coordinately determine the canine host range and specific antigenic and hemagglutination properties. J Virol 66, 6858–6867.
    [Google Scholar]
  4. Dorsch, S., Liebisch, G., Kaufmann, B., von Landenberg, P., Hoffmann, J. H., Drobnik, W. & Modrow, S. ( 2002; ). The VP1 unique region of parvovirus B19 and its constituent phospholipase A2-like activity. J Virol 76, 2014–2018.[CrossRef]
    [Google Scholar]
  5. Erdman, D. D., Durigon, E. L., Wang, Q. Y. & Anderson, L. J. ( 1996; ). Genetic diversity of human parvovirus B19: sequence analysis of the VP1/VP2 gene from multiple isolates. J Gen Virol 77, 2767–2774.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1989; ). phylip – Phylogeny inference package (version 3.2). Cladistics 5, 164–166.
    [Google Scholar]
  7. Kamstrup, S., Langeveld, J., Bøtner, A. & 8 other authors ( 1998; ). Mapping the antigenic structure of porcine parvovirus at the level of peptides. Virus Res 53, 163–173.[CrossRef]
    [Google Scholar]
  8. Martinez, C., Dalsgaard, K., López de Turiso, J. A., Cortés, E., Vela, C. & Casal, J. I. ( 1992; ). Production of porcine parvovirus empty capsids with high immunogenic activity. Vaccine 10, 684–690.[CrossRef]
    [Google Scholar]
  9. Mengeling, W. L. ( 1999; ). Porcine parvovirus. In Diseases of Swine, 8th edn, pp. 187–200. Edited by B. E. Straw, S. D'Allaire, W. L. Mengeling & D. J. Taylor. Ames, IA: Blackwell Science/Iowa State University Press.
  10. Ranz, A. I., Manclús, J. J., Díaz-Aroca, E. & Casal, J. I. ( 1989; ). Porcine parvovirus: DNA sequence and genome organization. J Gen Virol 70, 2541–2553.[CrossRef]
    [Google Scholar]
  11. Shackelton, L. A., Parrish, C. R., Truyen, U. & Holmes, E. C. ( 2005; ). High rate of viral evolution associated with the emergence of carnivore parvovirus. Proc Natl Acad Sci U S A 102, 379–384.[CrossRef]
    [Google Scholar]
  12. Simpson, A. A., Hébert, B., Sullivan, G. M., Parrish, C. R., Zadori, Z., Tijssen, P. & Rossmann, M. G. ( 2002; ). The structure of porcine parvovirus: comparison with related viruses. J Mol Biol 315, 1189–1198.[CrossRef]
    [Google Scholar]
  13. Soares, R. M., Cortez, A., Heinemann, M. B., Sakamoto, S. M., Martins, V. G., Bacci, M., Jr, De Campos Fernandes, F. M. & Richtzenhain, L. J. ( 2003; ). Genetic variability of porcine parvovirus isolates revealed by analysis of partial sequences of the structural coding gene VP2. J Gen Virol 84, 1505–1515.[CrossRef]
    [Google Scholar]
  14. Strassheim, M. L., Gruenberg, A., Veijalainen, P., Sgro, J.-Y. & Parrish, C. R. ( 1994; ). Two dominant neutralizing antigenic determinants of canine parvovirus are found on the threefold spike of the virus capsid. Virology 198, 175–184.[CrossRef]
    [Google Scholar]
  15. Swofford, D. L. ( 1993; ). paup: phylogenetic analysis using parsimony. Illinois Natural History Survey, Champaign, IL, USA.
  16. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal_w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  17. Truyen, U. A., Gruenberg, A., Chang, S.-F., Obermaier, P., Veijalainen, P. & Parrish, C. R. ( 1995; ). Evolution of the feline-subgroup parvoviruses and the control of canine host range in vivo. J Virol 69, 4702–4710.
    [Google Scholar]
  18. Vasudevacharya, J. & Compans, R. W. ( 1992; ). The NS and capsid genes determine the host range of porcine parvovirus. Virology 187, 515–524.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.81086-0
Loading
/content/journal/jgv/10.1099/vir.0.81086-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error