1887

Abstract

Hantavirus cell entry is promoted by its envelope glycoproteins, Gn and Gc, through cell attachment and by fusion between viral and endosomal membranes at low pH. However, the role of Gn and Gc in receptor binding and cell fusion has not yet been defined. In this work, a sequence presenting characteristics similar to those of class II fusion peptides (FPs) of alphavirus E1 and flavivirus E proteins is identified within the hantavirus Gc glycoprotein. A three-dimensional comparative molecular model based on crystallographic data of tick-borne encephalitis virus E protein is proposed for the Andes virus (ANDV) Gc ectodomain, which supports a feasible class II fusion-protein fold. experimental evidence is provided for the binding activity of the ANDV FP candidate to artificial membranes, as demonstrated by fluorescence anisotropy assays. Taken together, these results support the hypothesis that the Gc glycoprotein of hantaviruses and of other members of the family directs the viral fusion activity and that it may be classified as a class II viral fusion protein.

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2005-11-01
2019-12-15
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vol. , part 11, pp. 2937 – 2947

Sequence alignment of ANDV Gc and TBEV E proteins used as input for three-dimensional model building

Molecular-dynamic trajectory analyses

Comparison of the location of cystein residues and disulfide bridges within crystallographic structures and the ANDV Gc model

The best five hits of the threading program 3D-PSSM for ANDV Gc

The best five hits of the threading program LOOPP for ANDV Gc

The best five hits of the threading program FUGUE for ANDV Gc



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