RT Journal Article SR Electronic(1) A1 You, Jaehwan A1 Dove, Brian K. A1 Enjuanes, Luis A1 DeDiego, Marta L. A1 Alvarez, Enrique A1 Howell, Gareth A1 Heinen, Paul A1 Zambon, Maria A1 Hiscox, Julian A.YR 2005 T1 Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein JF Journal of General Virology, VO 86 IS 12 SP 3303 OP 3310 DO https://doi.org/10.1099/vir.0.81076-0 PB Microbiology Society, SN 1465-2099, AB The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81076-0